RESUMO
Background: thalassemia is accounted as the most common hereditary anemia through our region. Due to abnormality in synthesis of globin chains in red blood cell resulted from this disease, these globules have not natural life and then will die immediately. Appropriate therapy for this disease includes a regular monthly blood injection. However, in the approach patients will inevitably confront with side effects particularly iron overloads and iron sediment along tissue of body critical organs including heart, ductless glands and liver. Examining more research examples, by the study it attempted to determine more precisely plenty of adenoidal abnormality between the Iranian thalassemia patients for pre diagnosis and offering necessary medical measures
Materials and Methods: this was a descriptive research and included all the patients suffering from thalassemia major referring to medical centers linked with the Iranian blood transition institute. Census applied for sampling from all the patients. Initially, reports of adenoidal experiments as well as other associate parameters provided from medical folders
Results: from 437 understudy thalassemia patients, sexual mutation rate [SMR] was investigated along various age groups. There was no difference in those patients who, suffered from intermediate thalassemia and there was no relation between various age groups in the case of SMR classification [P=0/00]. Average of ferritin had no statistic difference among al 5 groups [P=0/6]
Conclusion: comparison of results between present research and similar studies conducted through Iran and those performed in abroad on growth abnormalities and puberty in thalassemia patients show that subject patients of this study statistically suffer from fewer difficulties than in case of patients in other studies
RESUMO
Background: Salmonella is the most important diarrheagenic pathogens, which can cause food borne disease where the main route of transmission among human is through contaminated meat and poultry foods. Its symptoms can be diarrhea, fever, vomiting and sometimes bloody diarrhea. For it's importance, it is essential to be identified and characterized by more precise methods such as molecular techniques. The aim of this study was to consider sensitivity of PCR-Ribotyping method for identification of Salmonella spp
Materials and methods: in this study our samples were Salmonella strains, which were isolated from patients with diarrhea. Their DNA was extracted by phenol/ Chloroform method. We did PCR-Ribotyping method with P1, P2 primers for 16S-23SrRNA gene. At last PCR-products run on 1.8% agarose gel in 120V for 90 min. the analysis was done after Ethidium bromide staining
Results: all the 40 strains containing paratyphi A, B, C and D serotypes also, serotype typhi contained 5 bands ranging 700 to 2500 bp
Conclusions: according to the results we can say that PCR-Ribotyping method has the highest sensitivity for identification of genus Salmonella but it is not suitable for Serotyping of Salmonella strains