Purification and cytotoxicity of glycoprotein isolated from Dendrobium huoshanense / 中国中药杂志

Dendrobium huoshanense is a rare traditional Chinese medicinal herb, and the anti-tumor activity of its polysaccharides is a research hotspot in traditional Chinese medicine resources domain. This study aims to explore the material basis for the anti-tumor activity of polysaccharide. D. huoshanense was used as raw material in the experiment, and the different protein components were obtained through low salt solution extraction and ammonium sulfate fractional precipitation. Then glycoprotein components were determined by SDS-PAGE electrophoresis staining, and were further isolated and purified by DEAE ion column and Sephadex gel column. At the same time, MTT assay was used in detecting the cytotoxicity of different products on HepG2 cells in vitro. As a result, three kinds of glycoprotein components RG1, RG2, RG3 with relative molecular mass of 22.5, 19.8, 15.6 kDa were gained, and the IC₅₀ of three compounds on human liver cancer cell HepG2 was 534.23 mg•L⁻¹, meanwhile IC₅₀ of single glycoprotein component RG1, RG2 was 432.96, 413.91 mg•L⁻¹ respectively, and glycoprotein component RG3 had no cytotoxicity on HepG2 cells. All in all, the experiment results suggested that two kinds of glycoproteins components with relative molecular mass of 22.5, 19.8 kDa may be one of the material basis for anti-tumor activity of D. huoshanense polysaccharide, and they had a synergistic effect.

A Novel Bihomoflavanonol with an Unprecedented Skeleton from Pteridium aquilinum / 中草药·英文版

Objective: To seek the flavonoids with the unique structure and to investigate the chemical ingredients in the flavonoid-rich plant- Pteridium aquilinum. Methods: The 80% EthOH extract from the degreased powder of P. aquilinum was partitioned by petroleum ether, CHCl3, EtOAc, n-butanol, and water, respectively. The EtOAc fraction was sequentially subjected to silica gel column, repeated Sephadex LH-20 column, and preparative TLC to give a new compound. The antitumor activity of the novel flavonoid was primarily evaluated by MTT. Results: Compound 1, a biflavonoid with the unique structure named as pteridium III with an unprecedented bihomoflavanonol skeleton, was isolated from P. aquilinum. Compound 1 showed the in vitro antitumor activity against lung cancer cell NCI-H46, melanoma cell A375, and glioma cell U-7MG corresponding to the IC50 values of 22.9, 106.7, and 1540.5 μmol/L, respectively. No inhibition on gastric carcinoma SGC-7901 and prostatic carcinoma PC-3 was observed in the experiment. Conclusion: A rare bihomoflavononol derivative, pteridium III, is obtained from the plant, which could enrich our knowedge on the chemical structures of flavonoids and bioactive constituents in P. aquilinum. © 2013 Tianjin Press of Chinese Herbal Medicines.