Serum vaspin levels are associated with decreased insulin sensitivity in newly diagnosed type 2 diabetes mellitus in Bangladesh

The present study aimed to explore the relationship of circulating vaspin levels with insulin sensitivity and anthropometric factors. This study was conducted with 65 newly diagnosed type 2 diabetes mellitus [T2DM] patients with age-matched 65 healthy controls. Serum glucose was measured using glucose-oxidase method, lipid profiles by enzymatic end-point methods, and fasting insulin and vaspin levels were assessed with ELISA techniques. Homeostasis model assessment for insulin sensitivity [HOMA%S] and insulin secretory capacity [HOMA%B] were estimated from the fasting glucose and insulin levels using HOMA-CIGMA software. Fasting serum insulin [micro U/ml] was higher in the diabetic group than controls [16.0 +/- 7.9 vs. 10.9 +/- 3.3, respectively, p=0.0001]. The mean [ +/- SD] HOMA%S of the diabetics was significantly lower than that of the controls [48 +/- 31 vs. 76 +/- 55, respectively, p = 0.001]. The HOMA%B of the T2DM group was nearly 50% of that of the controls [71 +/- 40 vs. 131 +/- 46, respectively, p = 0.001]. The T2DM group exhibited significantly lower serum vaspin [ng/ml] levels than the controls [0.62 +/- 0.26 vs. 0.83 +/- 0.28, respectively, p = 0.001]. Vaspin levels were negatively correlated with waist circumference [r = 0.17, p = 0.043] and positively correlated with HOMA%S [r = 0.243, p = 0.007] among all of the participants. The association of serum vaspin with diabetes remained highly significant [p = 0.008] in binary logistic regression analysis performed after adjusting for the effects of confounders. Serum vaspin level is positively associated with insulin sensitivity and negatively correlated with serum glucose, BMI and waist-height ratio

Increased concentration of circulating visfatin associates with post-challenged hyperglycaemia and insulin resistance in IGT subjects

Objectives: The cytokine visfatin is increased in obesity and type 2 Diabetes; however, its role in the development of diabetes is still unsettled. The present study aimed to investigate the serum visfatin levels in prediabetic subjects

Methods: Seventeen subjects with Impaired Fasting Glucose [IFG], 44 Impaired Glucose Tolerant [IGT], 16 IFG-IGT and 51 healthy subjects were recruited. Fasting insulin and visfatin were measured using enzyme-linked immunosorbent assay [ELISA] techniques. The Insulin sensitivity Homeostasis Model Assessment [HOMA%S] and B-cell secretory capacity [HOMA%B] were estimated using HOMA-CIGMA software

Results: HOMA%B was significantly lower in IFG [p = 0.0001] and IFG-IGT [p = 0.001] subjects. HOMA%S in IGT [p = 0.0001] and IFG-IGT [p = 0.001] subjects were significantly lower compared to controls. The fasting serum visfatin [ng/ml] level was significantly higher in IFG [5.08 +/- 2.16, p = 0.0001], IGT [4.75 +/- 2.81, p = 0.0001] and IFG-IGT subjects [4.33 +/- 2.68, p = 0.013] compared to controls [2.60 +/- 1.2]. In binary logistic regression analysis, visfatin has found significantly associated with IFG [B = 0.198, p = 0.040], IGT [B = 0.162, p = 0.043] and IFG-IGT [B = 0.188, p = 0.044]. Visfatin was also found significantly correlated with postprandial serum glucose and blood pressure in IGT subjects. Frequency of IFG, IGT and IFG-IGT subjects increased with increasing visfatin concentrations

Conclusions: Serum visfatin appear to be associated with IFG, IGT and IFG-IGT. Postprandial serum glucose and blood pressure are positively associated with visfatin levels in IGT subjects

Association of serum free IGF-1 and IGFBP-1 with insulin sensitivity and insulin secretory defects in Bangladeshi type 2 diabetes mellitus

Serum free insulin like growth factors-1 [IGF-1] and insulin like growth factor binding protein-1 [IGFBP-1] may have an association with insulin resistance and B-cell secretory dysfunction. Present study is undertaken to explore the independent association of IGF-1 and IGFBP-1with two basic defects in type 2 diabetic patients in a Bangladeshi population. Sixty eight type 2 diabetes mellitus [DM] subjects are studied along with age-, sex- and BMI-matched 61 healthy controls without family history of diabetes or prediabetes. Insulin, free IGF-1 and IGFBP-1 are measured by the standard ELISA method. Insulin secretory capacity [HOMA B] and insulin sensitivity [HOMA S] are calculated using fasting glucose and fasting insulin by HOMA-CIGMA software. Fasting free IGF-1 and IGFBP-1 levels are not significantly different between the study groups. But the level of free IGF-1 is significantly higher [p = 0.03] in type 2 DM subjects compared to controls in the low BMI group [BMI

effects of diabetes oncatecholamine neurotransmitter mediating enzymedopamine-beta-hydroxylase and other biochemicalparameters in human serum

In Bangladesh weather, environment and pattern of life style are significantly different from those of other developed as well as developing countries. So this makes us curious to determine the serum Dopamine-p-Hydroxylase [DBH] activity, its cofactors and other biochemical parameters in Bangladeshi diabetic patients at different ages of both sexes to determine their changes with age. The study was done in six different age groups of both sexes namely Group I: 10-15 years, Group II: 15-25 years, Group III: 25-35 years, Group IV: 35-45 years, Group V: 45-55 years and Group VI: 55+ years. DBH activity was increased in Groups I, II and decreased in Group VI of both sexes. DBH activity was variable in all other groups. One of the cofactors, ascorbic acid was slightly decreased in all groups. Another cofactor copper was variable. Among the parameters of lipid profiles, only the triacylglycerol level increased in all groups. Other biochemical parameters were variable. From our studies, we concluded that diabetes affects the DBH activity, its cofactors and other biochemical parameters

Socio-demographic, health and food consumption pattern of the Hajjis: [preliminary observation]

A preliminary studs, covering 804 pilgrims from Arab and non-Arab] countries, was carried out in 1988 [1108 A.H.] in order to obtain some information on the socio-demographic health and food consumption pattern of the Hajjis. The study revealed that the Arab Hajjis were younger than non-Arabs, with the exception of the Nigerians; more health conscious and utilized health facilities more often than the others. It was further observed that the Arabs possessed a higher level of education and were diversified in their dietary habits as compared to those of non-Arabs. The importance of general health orientation is being emphasized