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Chinese Journal of Cardiology ; (12): 743-747, 2005.
Artigo em Chinês | WPRIM | ID: wpr-253073

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of oxidized low-density lipoprotein receptor 1 (LOX-1) on secretion of adhesive molecules mediated by ox-LDL in human umbilical endothelial cells (HUVECs).</p><p><b>METHODS</b>HUVECs with different concentration of ox-LDL (0, 10, 20, 50, 100 microg/ml) were incubated for 24 h, or HUVECs were pretreated with 250 microg/ml poly (I) or 250 microg/ml carrageenan for 2 h and then incubated with 50 microg/ml ox-LDL for another 24 h. Expression of LOX-1 was determined by realtime RT-PCR and Western blot. mRNA and protein of ICAM-1, VCAM-1 and E-selectin were examined by RT-PCR and Western blot respectively.</p><p><b>RESULTS</b>Incubation of HUVECs with ox-LDL (10-100 microg/ml) enhanced the expressions of LOX-1, ICAM-1 and E-selectin in a concentration-dependent manner (P < 0.01). On the contrary, ox-LDL did not affect the expression of VCAM-1 by HUVECs. The expression of LOX-1, ICAM-1 and E-selectin induced by ox-LDL were reduced in HUVECs pretreated with 250 microg/ml poly (I) or 250 microg/ml carrageenan for 2 h and then incubated with 50 microg/ml ox-LDL for 24 h. This showed that both poly (I) and carrageenan obviously decreased the expression of LOX-1, ICAM-1 and E-selectin induced by ox-LDL.</p><p><b>CONCLUSION</b>ox-LDL may upregulate the expression of LOX-1, ICAM-1 and E-selectin, and LOX-1 blocker may partly inhibit this upregulation. The results suggest that the expression of inflammatory molecules induced by ox-LDL in HUVECs is mediated by LOX-1.</p>


Assuntos
Humanos , Adesão Celular , Moléculas de Adesão Celular , Células Cultivadas , Selectina E , Metabolismo , Células Endoteliais , Metabolismo , Endotélio Vascular , Metabolismo , Molécula 1 de Adesão Intercelular , Metabolismo , Lipoproteínas LDL , RNA Mensageiro , Metabolismo , Receptores de LDL Oxidado , Metabolismo , Receptores Depuradores Classe E , Metabolismo , Veias Umbilicais , Biologia Celular , Molécula 1 de Adesão de Célula Vascular , Metabolismo
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