RESUMO
<p><b>BACKGROUND</b>Primary biliary cirrhosis (PBC) is a chronic and slowly progressive cholestatic liver disease characterized by destruction of the interlobular bile ducts and a striking female predominance. The aim of this study was to identify associations between estrogen receptor (ESR) gene polymorphisms with the risk of developing PBC and abnormal serum liver tests in a Chinese population.</p><p><b>METHODS</b>Thirty-six patients with PBC (case group) and 35 healthy individuals (control group) from the First Hospital of Jilin University were studied. Whole genomic DNA was extracted from all the participants. Three single-nucleotide polymorphisms (rs2234693, rs2228480, and rs3798577) from ESR1 and two (rs1256030 and rs1048315) from ESR2 were analyzed by a pyrosequencing method. Demographic data and liver biochemical data were collected.</p><p><b>RESULTS</b>Subjects with the T allele at ESR2 rs1256030 had 1.5 times higher risk of developing PBC than those with the C allele (odds ratio [OR] = 2.1277, 95% confidence interval [CI] = 1.1872-4.5517). Haplotypes TGC of ESR1 rs2234693, rs2228480, and rs3798577 were risk factors for having PBC. The C allele at ESR1 rs2234693 was associated with abnormal alkaline phosphatase (OR = 5.2469, 95% CI = 1.3704-20.0895) and gamma-glutamyl transferase (OR = 3.4286, 95% CI = 1.0083-13.6578) levels in PBC patients.</p><p><b>CONCLUSIONS</b>ESR2 rs1256030 T allele may be a significant risk factor for the development of PBC. Screening for patients with gene polymorphisms may help to make early diagnoses in patients with PBC.</p>
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Povo Asiático , Estudos de Casos e Controles , Receptor alfa de Estrogênio , Genética , Receptor beta de Estrogênio , Genética , Frequência do Gene , Genética , Predisposição Genética para Doença , Genética , Haplótipos , Genética , Cirrose Hepática Biliar , Genética , Polimorfismo de Nucleotídeo Único , Genética , Receptores de Estrogênio , GenéticaRESUMO
<p><b>OBJECTIVE</b>To further study the binding character of hepatitis B surface antigen (HBsAg) and beta 2-glycoprotein I (beta2GP I) and to explore whether beta2GP I plays an important role in the hepatotropism of hepatitis B virus.</p><p><b>METHODS</b>Using Western blot technique, we observed the binding character of the HBsAg with reduced and non-reduced beta2GP I.</p><p><b>RESULTS</b>rHBsAgs with reduced and non-reduced beta2GP I showed identical binding activity.</p><p><b>CONCLUSIONS</b>The binding activity of HBsAg is dependent on tandem residues, but not on conformational structures of beta2GP I. There is a specific binding between HBV and beta2GP I, which may play an important role in HBV infection and is one of the reasons of hepatotropism of HBV.</p>
Assuntos
Humanos , Hepatite B , Virologia , Antígenos de Superfície da Hepatite B , Metabolismo , Vírus da Hepatite B , Virulência , Proteínas do Envelope Viral , Sangue , beta 2-Glicoproteína I , SangueRESUMO
It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro.