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New Egyptian Journal of Medicine [The]. 2005; 32 (Supp. 3): 7-15
em Inglês | IMEMR | ID: emr-73848

RESUMO

Enteroviruses especially Coxsackie B viruses [CBVs] are responsible for approximately 50% of viral myocarditis cases. Coxsackie B viruses were inoculated in BGM cells for three successive passages. Viral capsid proteins were screened by SDS-PAGE, followed by Western blot. Electrophoretically separated proteins of CB2, CB4 in comparison to CA9, Echo9, and PV1 Sabin, showed common peptides particularly at the low molecular weight [ranging between 40 to 10 kDa]. The results of immunoblotting analysis using polyclonal antibodies raised against the whole Coxsackieviruses B viral particles did not reflect that all those bands are immunogenic, nevertheless those peptides could be used to raise specific antibodies which in turn could be useful in detecting viral circulating antigens


Assuntos
Infecções por Coxsackievirus/virologia , Proteínas do Capsídeo , Anticorpos Monoclonais , Western Blotting , Eletroforese em Gel de Poliacrilamida , Enterovirus Humano B
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