Effect of ethanol extract of flowers of Vitex Trifolia Linn. on CCL[4] induced hepatic injury in rats

Hepatoprotective activity of ethanolic extract of flowers of Vitex trifolia [Verbenaceae] was studied against CCl[4] induced hepatic injury in albino rats. The plant extract [EVT] at the dose of 200 mg/kg, p.o. showed a remarkable hepatoprotective activity. CCl[4] induced a significant rise in serum glutamate pyruvate transaminase [SGPT], serum glutamate oxaloacetate transaminase [SGOT], alkaline phosphatase [ALP], total bilirubin and gamma glutamate transpeptidase [GGTP]. Treatment of rats with EVT significantly [P<0.001] altered serum biomarker enzyme levels to near normal against CCl[4] treated rats. The activity of the extract was comparable to the standard drug, silymarin [100 mg/kg, p.o.]. Histopathological observations also revealed that treatment with EVT protected the animals from CCl[4] induced liver damage. The results indicate that the flowers of V. trifolia possess hepatoprotective activity on CCl[4] induced hepatic injury in rats

Studies of anticancer and antipyretic activity of Bidens pilosa check for this species in other resources whole plant

Screening of different extracts and fractions from the plant Bidens pilosa check for this species in other resources Linn. var. (Asteraceae) has been conducted using the in - vitro comet assay for anticancer and the antipyretic action; which was done with in - vivo models.The extract from whole plant was extracted with n - hexane;chloroform and methanol extract (E1 - E3).The extracts were fractioned by column chromatography method and fractioned with ethyl acetate; acetone and water (F1 - F3).All the extracts and fractions were tested for anticancer and antipyretic activity. Among extracts E1 shows remarkable anticancer activity and E3 bears maximum antipyretic activity. In the antipyretic activity; paracetamol was used as the standard test drug.The most promising material (LC50 1500 ?g / ml) was F1 ethyl acetate fractions of methanolic extract and methanolic crude extract of whole plants. However; little correlation was observed in the degree of antipyretic activity between the test drug and standard drug. In conclusion; the extract obtained from the whole plant of Bidens pilosa showed a significant cytotoxic effect to methanolic extract against Hela cells by in vitro method and showed a comparable antipyretic activity effect to paracetamol in rabbit pyrogen test