Results of Primary Closure and Excision of Pilonidal Sinus Disease

Objective: The aim of this analysis was to determine the results of excision and primary closure of pilonidal sinus disease. Study Design: A Prospective Study. Place and Duration: In the Surgical department of PIMS Hospital Islamabad for the one-year duration from May 2018 to May 2019. Methodology: Eighty cases were included for excision with primary closure. Individuals having recurrent pilonidal sinus disease and with acute pilonidal sinus, abscess were excluded from the analysis. All subjects were operated in prone position under general anaesthesia. To clean the sinus tract; Methylene blue dye was injected. Postoperative complications and recurrence were recorded. All patients were followed at the outpatient department for three months and then every three months. Results: Eighty patients who met the criteria of inclusion were underwent surgery. 68 were male of the 80 patients, the female was 12, and 34 years was the mean age. Six days was the mean hospital stay and three weeks was the average duration when patients return to their work. From 80 patients; 10 (12.5%) had wound infection and 3 from them had minor wound infection. The seroma formation was noted in 5 (6.25%) which was aspirated. Only 6 patients had a recurrence, representing a 7.5% overall recurrence rate. Conclusion: It is concluded that pilonidal sinus disease primary closure and excision are related to early wound healing, short hospital stay, reduced chance of recurrence and rapid return to work.

Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.

Study on quality and efficacy of commercial tylosin and doxycycline products against local isolates of mycoplasma in broilers

The present study was conducted to investigate the quality and efficacy of commercially available preparations of tylosin and doxycycline available in the local market at Peshawar for poultry. In vitro and in vivo, tests were conducted to check the quality of these antimicrobial drugs. In vitro quality control test was performed by High performance liquid chromatographic [HPLC] and micro dilution method. In vivo, efficacy of the test drugs was checked in broilers infected with Mycoplasma gallisepticum. Results of HPLC indicated that test drug-2 contains doxycycline hydrochloride within specified limits but contain high quantity of active ingredient [Tylosin tartrate 120%]. Recovery percentage of test drugs [3, 4, 5] were below the pharmacopoeial limit, which contained low quantity of tylosin tartrate [85%, 87.5%, 85%] respectively however, percent recovery of doxycycline were in the appropriate limits. All the tested drugs were effective against Mycoplasma gallisepticum and showed minimum inhibitory concentration [MIC] at 1.9micro g/ml. The in vivo result indicated that all tested drugs decreased morbidity and mortality in infected chicks. The birds treated with test drugs [3 and 5] showed mortality of 9.5%, which was slightly higher than the other test groups. The current study suggested that there are incidences of substandard drugs in Pakistan and the drug regularity authorities should take strict actions against the manufacturing companies

Attempts to clone Bacillus stereothermophillus N methylase gene

4.30 KB plasmid DNA was isolated from Bacillus stereothermophillus N. chromosomal and plasmid DNA were ligated in pBR 322 and pUC 18. They were transformed in different bacterial strains. But it was observed no hybrid DNA got transformed