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1.
China Journal of Chinese Materia Medica ; (24): 2199-2203, 2014.
Artigo em Chinês | WPRIM | ID: wpr-330321

RESUMO

ITS2 sequence was used as a barcode to identify herbal tea ingredient Plumeria rubra and its adulterants. Genomic DNAs from forty eight samples were extracted, the ITS2 sequences were amplified and sequenced bi-direstionlly, and then assembled and obtained using CodonCode Aligner. The sequences were aligned using ClustalW, the genetic distances were computed by kimura 2-parameter (K2P) model and the Neighbor-joining (NJ) phylogenetic trees were constructed using MEGA5.0. Results showed that the length of ITS2 sequence of P. rubra were 244 bp. The intra-specific genetic distances (0-0. 016 6) were much smaller than inter-specific ones between P. rubra and its adulterants(0.320 8-0.650 4). The NJ tree indicated that P. rubra and its adulterants could be distinguished clearly. Therefore, Using ITS2 barcode can accurately andeffectively distinguish herbal tea ingredient P. rubra from its adulterants, which providesa new molecular method to identify P. rubra and ensure its safety in use.


Assuntos
Apocynaceae , Classificação , Genética , Código de Barras de DNA Taxonômico , Métodos , DNA de Plantas , Genética , DNA Espaçador Ribossômico , Genética , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas , Química , Classificação , Flores , Química , Classificação , Dados de Sequência Molecular , Filogenia , Controle de Qualidade
2.
China Journal of Chinese Materia Medica ; (24): 423-426, 2014.
Artigo em Chinês | WPRIM | ID: wpr-287572

RESUMO

Volatile oil components and the contents and types of amino acid in spica of Prunella vulgaris were analysed by GC-MS and amino acid analyzer. Esters, fatty acids, aromatic hydrocarbon, ketone and several alcohol compounds were identified by mass spectrum comparison. In these ingredients, beta-ionone smelled aroma of cedar, raspberry, nerolidol showed weak sweet soft orange blossom flavor, neroli tasted sweet and fresh, nerolidol tasted sweet with light aroma of wood, hexadecanal showed a weak aroma of flowers and wax, alpha-sinensal had rich and fresh sweet orange flavor. To some extent, these types of aromatic substances can affect the taste of herbal tea or decoction made of Spica Prunellae. Among amino acids detected, natural amino acids accounted for a larger proportion, and those natural amino acids showed bitterness, slight bitterness, sourness (freshness), sweetness, slight sweetness, sourness (slight freshness). The results indicated that bitter and slightly bitter amino acids have the greatest impacts on the sense of Spica Prunellae.


Assuntos
Aminoácidos , Cromatografia Gasosa-Espectrometria de Massas , Óleos Voláteis , Prunella , Química , Paladar
3.
China Journal of Chinese Materia Medica ; (24): 3666-3671, 2013.
Artigo em Chinês | WPRIM | ID: wpr-291306

RESUMO

Prunella vulgaris is a perennial herb whose dry spike is used for medicine in China. It is also a very important traditional medicine. Current study was conducted to the biomass components factors of P. vulgaris in the different ecological environments for the development and protection. The results showed that the contribution factor of the production in different environments was significant differences. In favorable environment, the length of leaf, numbers of leaves, height, reproductive branch number of individuals gave the greatest contribution. In transitional habitats, the diameter of stem and spike, the length of leaf contributed the most. While in the undergrowth and dry hillside, the length of root, the width of leaf, the length of spike were the main factors for biomass.


Assuntos
Biomassa , China , Ecossistema , Folhas de Planta , Raízes de Plantas , Caules de Planta , Prunella
4.
China Journal of Chinese Materia Medica ; (24): 1748-1751, 2007.
Artigo em Chinês | WPRIM | ID: wpr-287905

RESUMO

<p><b>OBJECTIVE</b>Based on 'Back-tracking' method, identification and quality evaluation of complex traditional Chinese medicine (TCM) preparation of Baoji pills (BJP) were carried out by HPLC fingerprint analysis.</p><p><b>METHOD</b>HPLC-DAD fingerprint of BJP was conducted with Zorbax SB-C18 column and non-linear elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid at column temperature 30 degrees C and detective wavelengths of 250 nm and 283 nm. From the established chromatographic pattern of BJP, track backward to the corresponding crude herbal drugs in the formula, attribution ofmost peaks in the BJP fingerprint can be disclosed.</p><p><b>RESULT</b>The BJP HPLC fingerprint consisted of 44 peaks among which 35 peaks were assigned by parallel comparison with the fingerprint of the 10 corresponding crude drugs in the formula such as pueraria, pummelo peel, and magnolia bark, etc. and 22 peaks we reidentified by comparison with the chemical reference substances.</p><p><b>CONCLUSION</b>The established HPLC fingerprint represents the whole character of BJP, which enhanced the specialty for control and assessment of the product quality. It exemplified much more effective for quality control than selecting any marker for qualitative or quantitative testing target. And the Back-tracking' experimental method extended the study mentality for complex formula TCM products chromatographic fingerprinting analysis.</p>


Assuntos
Cromatografia Líquida de Alta Pressão , Métodos , Chrysanthemum , Química , Citrus , Química , Combinação de Medicamentos , Medicamentos de Ervas Chinesas , Química , Magnolia , Química , Medicina Tradicional Chinesa , Plantas Medicinais , Química , Pueraria , Química , Reprodutibilidade dos Testes
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