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1.
Chinese Journal of Oncology ; (12): 497-500, 2013.
Artigo em Chinês | WPRIM | ID: wpr-267513

RESUMO

<p><b>OBJECTIVE</b>To explore the molecular mechanism of miR-124 suppressing the proliferation and invasion of gastric cancer cells.</p><p><b>METHODS</b>SPHK1 3'UTR-luciferase vector was constructed and luciferase reporter gene assay was employed to examine the effect of miR-124 on luciferase activity. Human gastric cancer MGC-803 cells were transfected with miR-124 mimics, and then Western blot was performed to detect the expression of SPHK1 protein.</p><p><b>RESULTS</b>Luciferase reporter vector system confirmed that SPHK1 was a target gene of miR-124. Western blot showed that the expression of SPHK1 protein was inhibited by miR-124. After transfection of miR-124 mimics or SPHK1 siRNA for 12 h, 24 h and 48 h, respectively, MTT assay showed that the A values of the three groups were significantly different (P < 0.05), and it was in a time-dependent manner. After transfection of miR-124 mimics or SPHK1 siRNA for 24 h, transwell invasion assay showed that the number of transmembrane cells was 54.6 ± 8.3 in the SPHK1 siRNA group and 47.8 ± 6.6 in the miR-124 mimics group, both were significantly lower than 100.6 ± 11.3 of the control group (P < 0.05), indicating that SPHK1 siRNA can slow down the invasion of MGC-803 cells.</p><p><b>CONCLUSION</b>miR-124 can suppress the cell proliferation and invasion by targeting SPHK1 in gastric carcinoma.</p>


Assuntos
Humanos , Linhagem Celular Tumoral , Proliferação de Células , Vetores Genéticos , Luciferases , Genética , Metabolismo , MicroRNAs , Genética , Invasividade Neoplásica , Fosfotransferases (Aceptor do Grupo Álcool) , Genética , Metabolismo , RNA Interferente Pequeno , Genética , Proteínas Recombinantes , Genética , Metabolismo , Neoplasias Gástricas , Metabolismo , Patologia , Transfecção
2.
Chinese Journal of Pathology ; (12): 324-328, 2007.
Artigo em Chinês | WPRIM | ID: wpr-333893

RESUMO

<p><b>OBJECTIVE</b>To study the role of PTEN gene involved in the biological behavior of human gastric carcinoma cells and underlying molecular mechanisms.</p><p><b>METHODS</b>Gastric carcinoma cell line, SGC7901, was transfected with plasmid PBP-PTEN and stable high PTEN expression clones were selected by Western blot and cell immunohistochemistry screening. Cell proliferation rate and apoptosis index of transfected cells were investigated by growth curve analysis, colony-formation assay and flow cytometry (FCM). Expressions of vascular endothelial growth factor (VEGF), matrix metalloprotease-2 (MMP-2) and MMP-9 proteins in cell culture supernatant and cytoplasm were determined by ELISA, gelatin zymogram, Western blot and cell immunohistochemistry.</p><p><b>RESULTS</b>Stable clone with high level expression of PTEN was successfully established (PTEN-SGC7901). Cell doubling time of PTEN-SGC7901 was longer than that of the control cells (P < 0.05). The size and colony-forming efficiency of PTEN-SGC7901 cells deceased compared with those of the control. The relative colony-inhibition efficiency of PTEN-SGC7901 to SGC7901 (naïve untransfected) and PBP-SGC7901 (control vector transfected) cells were 69.8% and 64.8%, respectively. PTEN-SGC7901 clone had more cells at G1 phase (P < 0.05) compared with that of the control. However, the apoptosis index did not show significant differences among the three groups (P > 0.05). There were significantly less VEGF and MMP-9 protein expressions in the PTEN-SGC7901 culture supernatant and cytoplasm (P < 0.05). In contrast, the MMP-2 expression among three cell groups had no significant difference (P > 0.05).</p><p><b>CONCLUSIONS</b>PTEN expression suppresses the growth and proliferation of gastric carcinoma cell SGC7901, possibly through an inhibition of the expressions of VEGF and MMP-9.</p>


Assuntos
Humanos , Apoptose , Ciclo Celular , Linhagem Celular Tumoral , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Metaloproteinase 2 da Matriz , Metabolismo , Metaloproteinase 9 da Matriz , Metabolismo , PTEN Fosfo-Hidrolase , Genética , Metabolismo , Plasmídeos , Neoplasias Gástricas , Metabolismo , Patologia , Transfecção , Fator A de Crescimento do Endotélio Vascular , Metabolismo
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