DiferenciaciÓn de Candida albicans y Candida dubliniensis por técnicas de electroforesis en geles de almidón y de poliacrilamida / Oral Candida albicans and Candida dubliniensis differentiatioin by multilocus enzyme electrophoresis and sodium dodecylsulphate-polyacrylamide gel electrophoresis

Cinco cepas de Candida albicans y otras cinco de C. dubliniensis así como sus cepas-patrón fueron evaluadas mediante el empleo de técnicas de electroforesis en geles de almidón y de poliacrilamida. Después de las electroforesis y evaluación numérica, se obtuvieron dos taxa muy distintos, que pueden justificar el uso de MLEE y SDS-PAGE como métodos confiables para la diferenciación e identificación complementaria de C.dubliniensis.

Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species

Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved

Criteria for Candida albicans numerical analysis based on electrophoretic protein patterns

Even in normalized gels, some problems with the choice and position of protein bands always have hindered the processing of electrophoretic data. We have developed a way to establish which criterion best fits the necessities in order to maximize the similarity indexes for numerical analysis. Some repetitions of a Candida albicans strain were carried out in eleven different gels. After staining, the bands were scored in numbers within ranges of +/- n values with increases of one quarter steps (+/- 0.25 kDa, +/- 0.5 kDa, +/- 0.75 kDa, +/- 1.0 kDa, +/- 1.25 kDa) that will limit the possibility of variation for the same bands that will appear on the repetitions. Using this criterion, we have determined that values scored within +/- 1.25 kDa could optimize the minimum limiting value of similarity in different dendrograms built.

Evaluation of different dehydrogenases to recognize Candida species commonly isolated from human oral cavities

Electrophoresis of some dehydrogenases were carried out in order to establish relatedness degrees among five Candida species commonly isolated from oral cavity of humans, by numerical taxonomy methods. The obtained data revealed that some dehydrogenases are capable of distinguishing strains of different species, but most of these enzymes could not organize all strains in their respective clusters. Numerical classifications based on dehydrogenase polymorphism must be considered for surveys involving just one species of yeast genus, where this resource had already shown to be useful.