Analysis of hypothalamic-pituitary-adrenal axis function in type 2 diabetic patients with different levels of HbA1C / 中华内分泌代谢杂志

Hypothalamic-pituitary-adrenal(HPA)axis-related factors in patients with type 2 diabetes were studied according to different levels of HbA1C.It showed that HPA axis was normal in HbA1C≤ 7% group[ACTH (18.03±8.39)ng/L,blood cortisol(49.22±8.68)μg/L],hyperactive in 7%11% group with weak feedback regulation[ACTH(26.08±15.41)ng/L,blood cortisol(55.64±24.27)μg/L].These results suggest that HPA axis-related factors in type 2 diabetic patients are different with different grades of glucose metabolic turbulence.

Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).</p><p><b>METHOD</b>TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.</p><p><b>RESULT</b>TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.</p><p><b>CONCLUSION</b>The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.</p>

Effects of Rehmannia glutinosa oligosaccharides on proliferation of HepG2 and insulin resistance / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effects of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of HepG2 and insulin resistance.</p><p><b>METHOD</b>The HepG2 cells were divided into control group, rosiglitazone (3.4 mg x L(-1)) treated group and ROS (0.1-30 mg x L(-1)) treated group. The proliferation of HepG2 was detected by MTT method. Insulin resistant HepG2 cells model was induced by high concentration of insulin, then the effects of ROS on glucose consumption in insulin resistant HepG2 cells were investigated.</p><p><b>RESULT</b>In the middle glucose culture medium, the absorbance at 570 nm of HepG2 was increased by high concentration of ROS, and decreased by low concentration of ROS by using MTT method, a concentration-dependent manner. ROS increased glucose consumption in HepG2 cells, and showed a better effect at the dose of 10 mg x L(-1). ROS promoted the glucose consumption in insulin resistance of HepG2 cells, improved the sensitivity of insulin resistance of HepG2 cells to insulin.</p><p><b>CONCLUSION</b>High concentration of ROS can promote the proliferation of HepG2, and however low concentration of ROS inhibits the proliferation of HepG2. ROS can significantly improve insulin resistance of HepG2 cells induced by high insulin.</p>

Research on isolation and purification of total iridoid glycosides from herbal Lamiophlomis rotata / 中国中药杂志

<p><b>OBJECTIVE</b>Enriching the hemostatic active part total iridoid glycosides from the aqueous extract of herbal Lamiophlomis rotata.</p><p><b>METHOD</b>First derivative spectrophotometry was used as the detection method and the content of total iridoid glycosides was used as tracking target. The abilities of absorption and anti-absorption of ten different types of macroreticular resin were compared, after being used to absorb the iridoid glycosides from aqueous extract of herbal L. rotata. The best purification process was optimized.</p><p><b>RESULT</b>With the optimized purification process, the content of total iridoid glycosides was 67.53%.</p><p><b>CONCLUSION</b>XDA-1 macroreticular resin has good ability of absorption and anti-absorption for iridoid glycosides and 50% ethanol was the best solvent for anti-absorption. The macroreticular resin could be reused.</p>

Effects of Rehmannia glutinosa oligosaccharides on proliferation of 3T3-L1 adipocytes and insulin resistance / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the influence of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of 3T3-L1 adipocytes and insulin resistance.</p><p><b>METHOD</b>3T3-L1 preadipocytes were cultured, the proliferation of 3T3-L1 preadipocytes was detected by MTT method. Insulin resistant 3T3-L1 adipocytes cell model was induced by dexamethasone and the change of glucose concentration in cell culture was determined after ROS treatment.</p><p><b>RESULT</b>In the high glucose DMEM culture media, MTT method showed that the absorbance at 570nm of 3T3-L1 preadipocytes was increased and that of 3T3-L1 adipocytes was decreased. ROS significantly increased glucose consumption in 3T3-L1 preadipocytes and adipocytes culture in a concentration-dependent manner. ROS improved the sensitivity of 3T3-L1 adipocytes to insulin.</p><p><b>CONCLUSION</b>ROS can promote the proliferation of 3T3-L1 preadipocytes, inhibite the proliferation of 3T3-L1 adipocytes, and also, significantly improve insulin resistance induced by dexamethasone.</p>

Effects of thymectomy on glucose metabolism in rats and modulation of rehmannia glutinosa oligosaccharides on glucose metabolism in thymectomized rats / 中国药理学通报

AIM: To observe the changes of blood glucose level, plasma insulin level and liver glycogen content and regulatory effects of rehmannia glutinosa oligosaccharides (ROS) on glucose metabolism in thymectomized rats. METHODS: 1) Plasma glucose level, liver glycogen content and plasma insulin level of thymectomized rats were determined three, four, eight and fifteen month after thymectomy operation; 2) Effects of ROS (250 mg·kg-1, po, × 60 d) on spleencyte proliferation, blood glucose level, liver glycogen content, plasma insulin level and plasma corticosterone level in thymectomized rats were investigated seven month after thymectomy. RESULTS: From 8 months after thymectomy liver glycogen content and plasma insulin level increased in thymectomized rats, though no obvious changes in blood glucose level were found during the experiment; 2) Changes of glucose metabolism in thymectomized rats were largely reversed and normalized with ROS: Increased liver glycogen content turned to be normal, increased plasma insulin level, decreased corticosterone level and decreased splenocyte proliferation in thymectomized rats became normal. CONCLUSION: Some changes in glucose metabolism are induced by thymectomy in rats, which can be largely reversed by ROS.