Changes and clinical significance of serum interleukin 17,interleukin 18 and interleukin 18 binding protein in patients with aplastic anemia / 中国基层医药

Objective To explore the clinical significance of changes of serum.interleukin 17(IL -17), interleukin 18(IL -18)and interleukin 18 binding protein(IL -18BP)in patients with AA.Methods Serum IL -17,IL -18 and IL -18BP levels were measured by ELISA in 50 patients with AA and 52 health control.Results The levels of serum IL -17,IL -18 and IL -18BP in the AA group were (216.41 ±22.24)μg/L,(402.37 ± 59.76)ng/L and (8.79 ±2.61 )μg/L respectively,which were higher than those in the healthy control group of (90.36 ±9.78)μg/L,(136.48 ±20.25)ng/L and (2.01 ±0.68)μg/L(t =37.41,30.46,18.13,all P <0.01). And the levels of serum IL -17,IL -18 and IL -18BP in AAA group were (267.76 ±30.17)μg/L,(479.41 ± 68.76)ng/Land (11.72 ±3.29)μg/L,which were higher than those in the CAA group of (156.48 ±18.36)μg/L, (312.47 ±40.61)ng/L and (5.36 ±1.45)μg/L (t =15.73,10.43,8.92,all P <0.01).The serum level of IL -17 was positive correlation with the levels of IL -18 and IL -18BP in patients with AA (r =0.527,r =0.489,all P <0.05),the serum level of IL -18 was also positive correlation with the level of IL -18BP(r =0.731,P <0.01). Conclusion IL -17,IL -18 and IL -18BP may participate in the pathogenesis of AA,and there is a certain correla-tion,which may influence the process of AA.

The promoter effects of sodium butyrate on the malignant transformation of the immortalized esophageal epithelium induced by human papillomavirus / 中华病理学杂志

<p><b>OBJECTIVE</b>Study on the promoter effects of sodium butyrate in high or low dosages on carcinogenesis process, based on the immortalization of human fetal esophageal epithelium induced by human papillomavirus (HPV) 18E(6)E(7) genes.</p><p><b>METHODS</b>The immortalized esophageal epithelium SHEE was treated with high concentration of the sodium butyrate (80 mmol/L) and then with low concentration (5 mmol/L) for 8 weeks respectively. The cells were cultured continuously without sodium butyrate for 14 weeks. The morphology, proliferation and apoptosis of the cells were studied by phase contrast microscopy, immunohistochemistry and flow cytometry. The dead and the viable cells were assayed by fluorescent microscopy with Hoechst 33342 and Propidium iodide staining. Tumorigenesis of the cells was assessed by soft agar colony formation and by transplantation of cells into nude mice and SCID mice.</p><p><b>RESULTS</b>When cells were exposed to high concentration of sodium butyrate, cell death was increased leaving few live cells. When cells were cultured in the medium with low concentration of sodium butyrate, the first proliferative stage appeared. Removal of the butyrate caused the cell to enter a crisis stage with a long doubling time resembling senescent cells. After the crisis stage, the cells progressed to the second proliferation stage with continuous replication and atypical hyperplasia. At the end of the second proliferative stage, carcinogenesis of the cells appeared with large colonies in soft-agar and tumor formation in transplanted SCID mice and nude mice.</p><p><b>CONCLUSIONS</b>The malignant change of the immortalized epithelium by the effects of sodium butyrate is the consequence of a two-stage mortality mechanism: cells death by butyrate cytotoxicity and cell crisis by abrogation of sodium butyrate. These data reveal that in high dosage, sodium butyrate induces cell death and in low dosage, it induces cell proliferation, which emphasizes the importance of butyrate as a promotor of carcinogenesis.</p>

Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the effect of selenium (Se) and iodine (I) and the compound of both on the proto-oncogenes c-fos and c-jun mRNA and their protein expression in the cultured rat hippocampus neurons.</p><p><b>METHODS</b>Using the technique of serum free hippocampus neuron culture, different doses of Se and I and Se + I compound were added into the medium. The expression of the mRNA of c-fos, c-jun in hippocampus neurons cultured for 1, 3, 5, 7 and 10 d were studied using both in situ hybridization and SABC immunohistochemical technique.</p><p><b>RESULTS</b>Both Se and I could enhance the expression of c-fos, c-jun mRNA and their proteins, especially the combination of I and Se able to give a remarkable effect on c-jun mRNA expression.</p><p><b>CONCLUSIONS</b>Se and I may effect the expression of both c-fos and c-jun mRNA, especially the c-jun mRNA and its protein of hippocampus neurons, and thus may effect the differentiation and development of neurons.</p>