RESUMO
To determine the hepatoprotective effect of Aqueous extract of Stem Bark of Berberis Lycium Royale in Isoniazid [INH] induced hepatotoxicity in mice. Experimental study. The study was conducted from 7[th] April 2014 till 7[th] May 2014 at animal house of National Institute of Health, Islamabad. Fifty six male albino Balb/C mice were randomly divided into four groups i.e Group I: Control group [C], Group II: Drug treated group [D], Group III: Low Aqueous group [LA], Group IV: High Aqueous group [HA] of 14 mice each. INH [50 mg/kg] was given to induce hepatotoxicity in all groups except control group [C]. Aqueous extract of stem bark of Berberis Lycium Royle was given in low doses [150 mg/kg] to Low Aqueous group [LA] and in high doses [200 mg/kg] to High Aqueous group [HA]. Blood samples were collected at day [0,15[th] and 30[th]] for estimation of Liver function tests [LFTs]. At the end samples for liver histopathology were taken. INH produced severe hepatotoxicity as depicted by raised LFT's and severe steatosis, hepatocytic ballooning and inflammation. In animals of Low Aqueous group [LA] and High Aqueous group [HA], serum levels of biomarkers were decreased and their liver sections showed improved histological picture but the reduction in toxic effects were more pronounced in animals treated with high Aqueous group. [HA]. Aqueous extract of stem bark of Berberis Lycium Royale has more significant hepatoprotective potential in high doses as compare to low doses