RESUMO
The present investigation was conducted to study the antibacterial effect of aqueous, ethanolic and ethyl acetate extracts of Teucrium polium plant on the bacteria isolated from urine samples of those with UTI and to compare it with the effect of commonly used antibiotics in treating UTIs. The antibiotic resistance of 147 strains of bacteria causing UTIs to the antibiotics selected through Kirby-Bauer disk diffusion method was determined. In the meantime, the aqueous, ethanolic and ethyl acetate extracts of T. polium plant were prepared. The antibacterial activity of these extracts was examined using Disk Diffusion Method. Finally, Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] of antibacterial were determined using serial dilution method. T. polium extracts were merely effective in enterococcus and pseudomonas bacteria. In general, the MIC rate of aqueous extract in enterococcus was 1.25-5 mg/ml. The MIC rate of ethanolic extract for enterococcus was calculated as 10 mg/ml. The MIC of aqueous and ethyl acetate extracts for pseudomonas bacteria were achieved as 5 and 20 mg/ml, respectively. The MBC contents of aqueous and ethyl acetate extracts of teucrium for pseudomonas bacteria was 10 mg/ml in aqueous and 20 mg/ml in ethyl acetate extracts. The MBC content of extracts for enterococcus bacteria were 10 mg/ml in aqueous extract and 20 mg/ml in ethanolic extract. T. polium extract can be effective in some bacteria causing urinary tract infection, especially enterococcus
RESUMO
Tuberculosis is a global public health problem in the world. Microscopy of sputum smears is the most widely used method for diagnosing tuberculosis. However, many patients are smears negative for acid fast bacilli. Regarding the pathogenesis of the disease, the effectiveness of interferon-gamma [IFN-gamma] in bronchoalveolar fluid was investigated for the disease diagnosis. This descriptive study was performed at the Ali Ibn-e-Abitaleb hospital, Zahedan, between 2010 and 2012, to assess the role of IFN-gamma level in bronchoalveolar lavage in distinguishing tuberculosis from other pulmonary diseases. In patients who required fiberoptic bronchoscopy as indicated, bronchoalveolar lavage was analyzed in terms of smear acid-fast staining and cytology. The participants were divided into TB patients group [the BK smear of bronchoalveolar fluid or the culture was positive] and pulmonary non-TB patients group [the smear was negative]. Yet non-TB disease was definitively diagnosed by other means, as well. The fluid in each group was examined in terms of IFN-gamma. Then, Mean IFN-gamma levels in BALF were measured in these groups and then compared with each other. Eighty eight patients were enrolled in the study among which, 31 cases had TB and 57 patients suffered from pulmonary non-TB disease. Mean IFN-gamma was 2.85 +/- 4.17 pg/mL in pulmonary TB patients and 2.21 +/- 1.21 pg/mL in pulmonary non-TB patients. Lack of significant differences between the two groups in IFN-gamma indicate that this factor is not suitable for diagnosis of tuberculosis and differentiating it from other pulmonary diseases