RESUMO
Acute myeloid leukemia [AML] is defined as leukemic blast reproduction in bone marrow. Chromosomal abnormalities form different subgroups with joint clinical specifications and results. t[8;21][q22;q22] and inv[16][p13;q22] form core binding factor- AML [CBF-AML]. c-kit mutation activation occurs in 12.8-46.1% of adults with CBF leukemia. These mutations occur in 20-25% of t[8;21] and 30% of inv[16] cases
Methods: In this systematic review, we searched different databases, including PubMed, Scopus, and Embase. Selected articles were measured based on the inclusion criteria of this study and initially compared in terms of titles or abstracts. Finally, articles relevant to the subject of this review were retrieved in full text. Twenty-two articles matched the inclusion criteria and were selected for this review
Results: In this study, c-kit mutations were associated with poor prognosis in AML patients with t[8;21] and inv[16]. In addition, these mutations had better prognostic effects on AML patients with inv[16] compared with those with t[8;21]
Conclusion: According to the results of this study, c-kit mutations have intense, harmful effects on the relapse and white blood cell increase in CBF-AML adults. However, these mutations have no significant prognostic effects on patients
RESUMO
Background and Aims: multiple myeloma is a malignant proliferation of plasma cells derived from a single clone. The tumor, its products and the host response lead to organ damages. Some factors that are responsible in its pathogenesis are recognized. As FMS like Tyrosine Kinase 3 receptor [FLT3] mutation has been proved as a determining factor in leukemic patients; the goal of this study was to find association of FLT3 internal tandem duplication [ITD] and FLT3 tyrosine kinase domain [TKD] mutations with multiple myeloma
Materials and Methods: this case-control study was conducted on 60 paraffinembedded bone marrow biopsies [30 multiple myeloma and 30 normal bone marrow specimens] in the pathology departments of Ghaem and Imam Reza hospitals in Mashhad. After sections preparation, DNA was extracted and two PCR reactions were set up for detection of FLT3/ ITD and FLT3/TKD mutations
Results: the Mean age of samples was 64+/-10 years. No FLT3 mutations were detected in multiple myeloma patients
Conclusion: our findings showed that occurrence of FLT3 mutations seem unusual in multiple myeloma
RESUMO
Background and Aims: human Immunodeficiency virus [HIV] is spreading rapidly among the people worldwide. Infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome. Early HIV detection is dependent on antibody screening against virus by enzyme linked immunosorbent assay [ELISA]. Some confirmatory tests such as western blot and recombinant immunoblotting assay [RIBA] are used to verify viral infection. Many of the confirmatory test results are indeterminate. The aim of this study was to compare the frequency and patterns of indeterminate results of confirmatory tests in two groups; blood donors and patients with high risk behaviors in the northeast of Iran
Materials and Methods: this is a cross-sectional study from October 2009 to March 2014. A total number of 1055 serum samples with previous positive HIV ELISA test history were tested in our laboratory, some by RIBA and some by western blot method
Results: most of the indeterminate results belonged to blood donors and western blot analysis. The most reacting band was P24 in both methods and groups
Conclusion: RIBA assay is more sensitive and reliable than western blot, but it is necessary to use other supplementary tests with less indistinctive results. It is necessary to pay attention to HIV glycoprotein reactivity in some methods, too