RESUMO
Objective:To establish a HPLC method for the simultaneous determination of adenosine,genistein and 5-hydroxymel-lein in Xylarin nigripes powder.Methods:The chromatographic separation was carried out on a Pntulips@BP-C18column (250 mm× 4.6 mm,5 μm) at 35℃. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution at a flow rate of 1.0 ml ·min-1,and the detection wavelength was 254 nm. Results:The linear range of adenosine was 0.162-1.62 μg(r=0.999 2). The average recovery was 98.12% and the RSD was 1.32% (n=6). The linear range of genistein was 0.02-0.2 μg(r=0.999 9). The average recovery was 98.89% and the RSD was 1.10% (n =6). The linear range of 5-hydroxymellein was 0.022-0.22 μg(r =0.999 9). The average recovery was 99.87% and the RSD was 0.98% (n=6). Conclusion:The method can be used to determine the contents of adenosine,genistein and 5-hydroxymellein,which provides the basis for the quality control and the further research of Xylaria nigripes.