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Aim A UVA-induced apoptotic model of HaCaT cells was established to investigate the impact of UVA on c-jun/cyclooxygenase-2(COX-2)and explore related molecular mechanism of the polypeptide from Chlamys farreri(PCF)protecting HaCaT cells from UVA-induced apoptosis.Methods Cells were divided into five groups:control group,UVA model group,UVA+5.69 mmol?L-1 PCF group,UVA+2.84 mmol?L-1 PCF group,UVA+1.42 mmol?L-1 PCF group.Expression level of c-jun was assayed by Real-Time PCR and Western blot.RT-PCR and Western blot analysis were used to determine the mRNA and protein levels of COX-2.Using agarose gel electrophoresis,the effects of PCF and COX-2 inhibitor celecoxib on UVA-induced apoptosis were also investigated.Results PCF and celecoxib had inhibitory effect on 8 J?cm-2 UVA-induced apoptosis of HaCaT cells.COX-2 mRNA and protein levels increased after UVA radiation and the discrepancy was significant compared with control group(P
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To explore new teaching method of functional experiment,we opened the functional laboratory to encourage students' creativity and train their practical ability and develop their innovative ability.The experiment teaching innovation was attempted and discussed the functional speciality.
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AIM To investigate the role and mechanism of a novel antiepileptic drug topiramate on amygdala kindling in rats. METHODS The effects and mechanism of topiramate on kindling were examined by the establishment of amygdala kindling model and combination with other drugs. The influence of topiramate on seizures induced by semicarbazide hydrochloride(SCZ) was also observed. RESULTS Topiramate (50～200 mg?kg -1 ,ig) dose-dependently inhibited the seizure severity in amygdala kindling ( P
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This study reported the protective effects of poly-saccharide sulphate (PSS) on experimental model of myocardial infaction by high positioned double - ligation of the anterior descending left coronary artery in rabbits, and on ischemic injury of myocardium by intraperi-toneal injection of isoprenaline in rats. The results showed that PSS could decrease the infarct sizes of rabbits model expressed by ECG mappingof ∑ST segment, NST and NQ, and reduce the activities of CPK in serum. It also showed that PSS reduced the severity of myocardial necrosis and the activies of CPK in serum of the experimental rats . It suggested that PSS have the protective effects on ischemic injury of myocardium.