Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the relationship between the variation of chloroplast DNA gene sequences and the geographical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources.</p><p><b>METHOD</b>PCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 populations of P. capitatum.</p><p><b>RESULT</b>The psbA-trnH gene sequence of P. capitatum from different populations was 402 bp in length, there were 6 variable sites. TrnL-F gene sequence was 875 bp, there were 5 variable sites. The clusters diagram by UPGMA method showed that P. capitatum groups in Yunnan and Guizhou existed a considerable variation.</p><p><b>CONCLUSION</b>P. capitaturni which is located in the east of Yunnan and the west of Guizhou is helpful of screening the germplasm resources.</p>

Genetic diversity of Polygonum capitatum from Guizhou populations by ISSR markers / 中国中药杂志

<p><b>OBJECTIVE</b>To detect genetic diversity of 48 population of Polygonum capitatum in Guizhou province.</p><p><b>METHOD</b>The genetic diversity of 48 representational populations of P. capitatum including 240 individuals had been investigated by ISSR marker technique.</p><p><b>RESULT</b>The genetic diversity had been revealed as follow: A total of 8 293 bands were produced in 240 individuals, of which 7 962 bands were common in the 48 population. The value of the average percentage of polymorphic bands (PPB) was 79.09%, Nei's genetic diversity index (H(e)) was 0.245 8, Shannon's information index (I) was 0.396 2, and genetic differentiation index (G(st)) was 0.238 0 at population level, respectively. The genetic differentiation index (G(st)) was 0.072 2, genetic differentiation coefficient by Shannon's diversity (I(st)) was 0.044 2 within the population levels. Groups cluster analysis based on the UPGMA method indicated that although the 48 populations could be divided into 3 groups and the P. capitatum seed sources. The groups cluster showed that a cross clustering of P. capitatum between the southwest and southeast populations in Guizhou province, and no significant correlation was found between geographical and genetic distance among them.</p><p><b>CONCLUSION</b>The genetic diversity of P. capitatum is relatively high at the population levels, while low within the population levels, a significant degree of genetic differentiation occurs among the populations. The groups cluster analysis indicated they has not apparent genetic variation in regional pattern between the place of origin populations and the migrate populations.</p>