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OBJECTIVE: To establish an LC-MS-MS method for the rapid analysis of the degradation compounds of vorionazole. METHODS: The degradation compounds and the control substance of vorionazole were compared in respect of relative molecular mass,fragments,spectra characteristics using LC-MS-MS technology. RESULTS: Vorionazole and its degradation compounds could be effectively isolated. The two main degradation compounds were the isomers of 4-ethyl-5-fluoropyrimidine. CONCLUSION: The analytic method is rapid,sensitive,and specific,and it can be applied to predicate the structure of the degradation compounds of vorionazole.
RESUMO
Objective To supply evidence for determining the optimal collecting period for Sambucus chinese.Methods A RP-HPLC method was established to determine chlorogenic acid contents from Sambucus chinese in various collecting periods.The chromatographic conditions were as follows: Hypersil-ODS2(250 mm? 4.6 mm,5 ? m)column,acetonitrile-1 % formic acid aqueous solution(9 ∶ 91) as mobile phase with a flow rate of 1.0 mL? min-1,column temperature at 35 ℃ and the detection wavelength at 326 nm.Results Content of chlorogenic acid in the samples collected in May(pre-blossom period)was the highest and the content in leaf was higher than in the stem and root.Conclusion It is suggested that Sambucus chinese should be gathered before blooming when the leaves are in luxuriance.