Characteristics and clinical values of SPECT/CT whole-body bone scanning in detecting bone metasta-ses in patients with lung adenocarcinoma or squamous cell carcinoma / 国际肿瘤学杂志

Objective To investigate the clinical values and characteristics of whole body bone ima-ging (SPECT/CT)in detecting bone metastases in the preoperative patients with lung adenocarcinoma or squa-mous cell carcinoma for staging and determining the best treatment plan.Methods Eighty-two preoperative patients with primary pulmonary adenocarcinoma or squamous cell carcinoma performed 99 Tcm-MDP SPECT/CT whole-body bone imaging.One week before surgery,parts of positive lesions performed MRI scan.The differ-ence of the incidence of bone metastasis was analysed by χ2 test.Results In all 82 patients with lung cancer, there were 38 adenocarcinomas and 44 squamous cell carcinomas.Bone metastases were detected in 38 cases, the incidence rate was 46.3%.Of which,among lung adenocarcinoma,the incidence rate was 57.9% (22 /38),and the incidence rate was 36.4% (1 6 /44)in lung squamous cell carcinoma,and the difference was sta-tistically significant (χ2 =1 2.66,P =0.027).The most common area was bilateral ribs,followed by vertebra, pelvis,bones of the extremities and skull.Conclusion Lung adenocarcinoma compared with squamous cell carcinoma is prone to bone metastases,and bone metastases are more common in bilateral ribs.It has important value that whole body bone imaging in screening for bone metastases of pre-operative patients with lung cancer for staging and making the treatment plan.

Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma / 生物医学工程学杂志

This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.

Pharmacokinetics of 2% lidocaine superior laryngeal nerve block combined with 5% lidocaine topical anesthesia to endotracheal mucosa by thyrocricocentesis / 中华麻醉学杂志

Objective: To study pharmacokinetics of lidocaine during superior laryngeal nerve block (SLNB) combined with topical anesthesia to endotracheal mucosa by thyrocricocentesis(TCC). Method: Eight patients (ASA Ⅰ-Ⅱ grade)scheduled for general anesthesia and operation were performed bilateral SLNB with 2% lidocaine 2mg/kg and TCC with 5 % lidocaine 2mg/kg within 1 min,The internal jugular venous blood samples were taken at 1,5,10,20,30, 60,120 and 180 minutes after lidocaine administrations to determine lidocaine serum concentrations using a fluoresence polarization immunoassy. Pharmacokinetics were calculated with a procedure (3p87) by computer. Result: The pharmacokinetics could be described as two-compartment model. Absorption rates of lidocaine were fast,values of t_(1/2) Ka and Tpeak were 5.0?2.5 min and 7.5?2.1 min respectively。Maximal concentrations (Cmax) of lidocaine were 3.8?0. 6?g/ml, which were within safety ranges. Conclusion: It may be safe that 2% lidccaine 2mg/kg superior laryngeal nerve block is combined with 5% lidocaine 2mg/kg topical anesthesia to endotracheal mucosa by thyrocricocentesis.