Cyclical Variations of Blood Concentration of Phenytoin and Its Pharmacokinetics in Menstrual Epilepsy / 中国药房

OBJECTIVE:To explore the changes of serum phenytoin levels and its pharmacokinetics in menstrual epilepsy.METHODS:9cases of menstrual epilepsy patients who were treated with phenytoin were collected,whose blood concentra?tions of phenytoin in menstrual period and ovulation period were respectively determined by HPLC,pharmacokinetics study was performed in three of them.RESULTS:The mean serum phenytoin levels in menstrual period and ovulation period were(9.25?2.71)?g/ml and(13.33?3.22)?g/ml,respectively(P

The Percutaneous Permeability of Methoxypsoralen Liposomal Gel / 中国药房

OBJECTIVE:To study the percutaneous permeability of methoxypsoralen liposomal gel METHODS:The preparations were applied on the rat skin in vitro and in vivo with common gel and Mingbailing tincture as control,and the drug concentrations in the receiver,skin,blood and other organs were determined by RP-HPLC RESULTS:In vitro experiment,liposomal gel showed the lowest percutaneous permeation rate and the highest retention in skin In vivo studies illuminated that the skin depositions of drug encapsulated in liposome was(4 37?0 91) and (3 36?0 58) times those of the common gel and tincture respectively CONCLUSION:Methoxypsoralen liposomal gel showed an evident targeting effect on skin

Pharmacokinetics of Levofloxacin Lactate Eye-drops in Aqueous Humor of Rabbits / 中国药房

OBJECTIVE:To measure the concentrations of levofloxacin in aqueous humor after a single eye dripping of drug and calculate the pharmacokinetic parameters.METHODS:32 rabbits were divided into 8 groups.The rabbit eyeballs were enucleated to make a collection of aqueous humor at 0.125,0.25,0.5,0.75,1.0,2.0,3.0 and 4.0 hours after dripping.All measurements were performed with HPLC.RESULTS:In aqueous humor,Tmax:0.940h,Cmax:0.592?g/ml,T1/2(Ka):0.479h,T1/2 (Ke)0.919h.By using 3p87 pharmacokinetic program,the concentration-time curves were fitted as one-compartment op_en model.CONCLUSION:All the concentrations of levofloxacin at different times after dripping in aqueous humor exceeded the minimum inhibitory concentration for 90%(MIC90)of most bacterial pathogens.These results show that levofloxacin lactate eye-drops may be an effective antimicrobial agent for the treatment and prevention of intraocular infections as well as other ocular infections.

Affect of the sea horse Hippocampus japonicus on pituitary- gonadal axis in male rats / 中国海洋药物

We studied a species of Haima-the Sea Horse Hippocampus japonicus (SHHJ). After SHHJ was given to male rats orally for twenty days. The plasma concentration of testosterone,LH and FSH was assayed by RIA,and the histological change in seminal vesicles and prostate gland was observed. It has been showed that SHHJ can obviously elevate plasma level of testosterone and improve the histological change of seminal vesicles and prostate gland in castrated rats. but there was no affect in LH、FSH. SHHJ can obviously promoted the content of testosterone of plasma and can obviously enhance the weight of the normal rat's prostate gland,seminal vesicle.

Effect of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation / 中草药

Objective To study the effects of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation in vitro. Methods Superoxide anion free radical(O_2~?) induced by the auto-oxidation of pyrogallol and hydroxyl free radical(?OH) induced by Fenton system were determined by spectrophotometry.The inhibition on homogenate and mitochondrial lipid peroxidation,malondialdehyde(MDA),of rat liver induced by VC-Fe~(2+) system was measured by thiobarabituric acid(TBA) colorimetric method.Results Sinomenine could significantly scavenge O_2~? and ?OH,it's EC_(50) were 1.35 and 0.136 mg/mL respectively.Sinomenine could also remarkably inhibit the lipid peroxidation of rat liver homogenate and mitochondria induced by auto-oxidation and VC-Fe~(2+) system.The scavenging and inhibitory effect exhibited in a concentration-dependent manner.Conclusion The results indicate that sinomenine possesses the effect of scavenging oxygen free radicals and anti-lipid peroxidation,which shows the protection of liver mitochondria.

Identification of Herbs in Shenning Granules / 中药新药与临床药理

Objective To establish a method for the identification of herbs i n Shenning granules. Method Radix Astragali, Herb Leonuri and Rhizoma Chunxio ng were identified by TLC. Results Under the selected condition, the clear and distinguished spots were obtained. Conclusion The method is simple and eff ective and can be used to control the quality of the preparation.

Primary research of fingerprint of Danggui Injection / 中成药

AIM: To study the fingerprint of Danggui Injection (Radix Angelicae Sinensis). METHODS: HPLC with UV detector was used in analyzing the patterns of fingerprint of Danggui Injection. It was separated on C 18 column. The mobile phase consisted of (A) water ( 0.25% HAC) and (B) methanol using a linear gradient, and the detection wavelength at 246nm. The flow rate was at 1mL?min -1 . RESULTS: The fingerprint of Danggui Injection was set up and the fingerprint of Angelica sinensis showed a correlation. CONCLUSION: The research is helpful to the quality control of Danggui Injection.

Study of the Therapeutic Effect of8-methoxypsoralen Liposome Gel on Leukodermia in Guinea-pig Models / 中国药房

OBJECTIVE:To compare the therapeutic effects of8-methoxypsoralen(8-MOP)liposomal gel with other dosage forms of8-MOP in equal concentration on guinea-pig models of vitiligo.METHODS:The guinea-pigs were divided into normal control,model,8-MOP tincture,8-MOP gel and8-MOP liposome gel groups.The vitiligo model was established by chemical decoloration method in all groups except normal control group,then corresponding preparations were topically ap?plied.The therapeutic effects were compared and the melanogenesis of hair follicles,serum acetylcholinesterases and skin MDA contents were observed in all groups.RESULTS:8-MOP liposomal gel had better therapeutic effects on the animal models,and could improve the dermatic melanogenesis and acetylcholinesterase of the animal model,and also had effect on MDA of skin.The differences between8-MOP liposomal gel group and model group were highly significant(P

Studies on the Preparation and Quality Standards for Sinomenine Gel / 中国药房

OBJECTIVE:To develop a preparation and quality control method for sinomenine gel.METHODS:Sinomenine gel was prepared with carpol971as base.TLC and UV were employed for identification of sinomenine.Sinomenine was deter?mined by RP-HPLC.RESULTS:The TLC identification was highly specific and the spots were clear.Recovery was99.6%?1.3%.CONCLUSION:This paper provides a rapid,convenient and accurate method which is suitable for preparation and quality control for sinomenine gel.

An Exploration on the Prescription Composition Rules of the Compound Anti-influenza Preparations Made from Chinese and Western Medicines / 中国药房

The prescription composition rules and special features of compound anti-influenza preparationS made from Chinese and western medicines are explored in this paper. The problems in the prescription composition rules are also discussed.

Effects of Di-p-Cl-benzoyl-linesinine on experimental arrhythmia / 中国药理学通报

AIM The purpose is to study the effect of Di p Cl benzoyl linesinine on experimental arrhythmias. METHODS The models used were caused by aconitine, ouabain and CaCl 2 repectively. RESULTS Di p Cl benzoyl linesinine 5 mg?kg -1 could increase the threshold dose of ventricular ectopic(VE), ventricular tachyacardia(VT), ventricular fibrillation(VF) and cardiac arrest(CA) caused by aconitine in rats and that by ouabain in ginuea pigs; delay the onset time of arrhythmia and decrease the incidence of VE,VF and CA induced by CaCl 2 in rats. CONCLUSION Di p Cl benzoyl linesinine possesses extensive effects of antiarrhythmia on ventricle.

Effects of shenning on rats with membranous glomerulonephritis / 中国药理学通报

AIM To investigate effects of shenning on nephrotic rats. METHODS Adult male wistar rats were randomly devided into normal control group, pathological pattern group, high-dosage shenning group,middle-dosage group,low-dosage group,shenfukang group and prednisone group. Rats with membranous glomerulonephritis were induced by injection of cation bovine serum albumin into tail vein. The rats were treated respectively for 32 days by gavage. Urinary protein was observed once a week after 2 weeks. After 7weeks ,plasma albumin,renal histology,parameters of renal function and prostaglandins were examined. In addition TXB 2/6-Keto-PGF 1? in serum were also examined. RESULTS The urinary protein,serum cretinine level,BUN and TXB 2/6-Keto-PGF 1? can be reduced in shenning groups compared with pathological pattern group. Plasma albumin was significantly increased in high-dosage shenning group. CONCLUSION High-dosage and middle-dosage shenning can significantly decrease urinary protein in rats with membranous glomerulonephritis. High-dosage shenning can increase the plasma albumin level in rats with membranous glomerulonephritis.

Effects of liensinine on platelet aggregation and coagulability and thrombotic activity / 中国药理学通报

Aim To investigate the effects of Liensinine on platelet aggregation and coagulation function in rats,as well as the effect on experimental thrombosis.Methods Inhibition rates of platelet aggregation for Liensinine in vivo were determined by the model of platelet aggregation induced by adenosine diphosphate.Coagulation time of mice was measured by capillary vessel method,and bleeding time of mice was measured by tail-cutting method.The effects of Liensinine were also evaluated on prothrombin time(PT),activated partial thromboplastin time(APTT)and thrombin time(TT).The model of artery-vein bypass thrombosis and Chandler's model were established to observe the effect of Liensinine.Results The result showed that Liensinine 5 and 10 mg?kg-1 had significant effect on inhibition of platelet aggregation and markedly prolonged bleeding time,coagulation time,PT,APTT and TT.Liensinine 5 and 10 mg?kg-1 inhibited the artery-vein bypass and Chandler's thrombus in different degree,reduced the thrombus weight significantly (either wet ordry).Conclusion Liensinine exerts remarkable effect against thrombosis and possesses strong effect against platelet aggregation and coagulation.