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This study was aimed to discuss cellular mechanism of anti-liver fibrosis by acupuncture on acupoint and connective tissues. Rats were divided into five groups, which were the normal group, liver fibrosis model group (12 week), liver fibrosis model group(20 week), acupoint acupuncture group, and connective tissue acupuncture group. The observation was made on morphology of liver in various groups by HE stain and Mallory stain. Concentrations of TG, HA, LN and PC-Ⅲin serum were also detected. Mesenchymal stem cells (MSCs) were intervened by serum from each group to observe MSCs proliferation and differentiation. The results showed that the serum level of model group(12 week) was significantly different from other groups. And the same results were found in the inspection of morphology. In the normal group there were no cell expressing albumin. In other groups, MSCs can express albumin. It was concluded that the serum of normal group cannot make MSCs transform into hepatocyte-like cell. The serum of other groups can make MSCs transform into hepatocyte-like cell. It was concluded that both hepatic fibrosis and acupuncture can induce MSCs differentiation.
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Objective To study the effects of TGF-? 1 on the proliferation of hepatic stellate cell(HSC) and the interfering effect of serum containing Fufangbiejiaruanganfang (FFBJRGF ) drug in vitro. Methods TGF-? 1 was added to the culture of HSC in vitro and the proliferation of HSC was detected by MTT method. The effects of the serum containing different dosage FFBJRGF on the HSC proliferation was observed by using modified method of serum pharmacology of the traditional Chinese medicine and MTT method. Results The proliferation of HSC was decreased under adding exogenous TGF-? 1, which effect was presented at 72 hours after TGF-? 1 complement. The HSC proliferative reactions were obviously inhibited by adding sera containing high, moderate, low dosage of FFBJRGF as well as IFN-?serum compared with the normal control HSC culture (P
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Objective To investigate the effects of the serum containing Fufangbiejiaruanganfang (FFBJRGF) on cell cycle and apoptosis of hepatic stellate cells(HSC). Methods The influence of TGF-? 1 on cell cycle and rate of apoptosis of HSC in vitro was examined by using flow cytometer, furthermore, the effects of FFBJRGF drug sera on HSC were observed compared with IFN-? serum and normal rat serum controls. Results The numbers in phase of G 0 and G 1 were increased when HSCs were incubated by adding TGF-? 1, IFN-? serum and different dosages of FFBJRGF drug serum respectively, especially being predominant in the FFBJRGP groups. Meanwhile, the intervenient roles in phase of G 2?M?S of HSC were found in FFBJRGF drug serum treatments. Compared with normal serum control group, there were not notable effects on the cellular apoptosis of HSC in the different dosages of FFBJRGF drug serum groups as well as IFN-? group, however, TGF-? 1 could significantly increased HSC apoptosis rate. Conclusion Our results indicates FFBJRGF has anti-hepatic fibrosis efficiency through inhabiting the proliferative cycle of HSC, not up-regulating HSC apoptosis in vitro.
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Objective To investigate the mechanisns of motivating blood circulation and removing blood stasis decoction for anti- hepatic fibrosis. Methods The hepatic fibrosis rats were fed with motivating blood circulation and removing blood stasis decoction, PC- NA and a-SMA expression of liver tissue were observed by means of immunohistochemical technique Results Motivating blood circula- tion and removing blood stasis decoction inhibited ?-SMA expression of HSC compared with the self-restore grounp (P