Clinical observation of belimumab in the treatment of 12 children with active lupus nephritis / 中国小儿急救医学

Objective:To analyze the clinical data of children with active lupus nephritis(LN) with poor first-line treatment and further treatment with belimumab, and explore the efficacy and safety of belimumab in the treatment of children with LN, so as to provide experience and guidance for clinical treatment of children with LN.Methods:From August 2020 to September 2021, 12 children with LN whose systemic lupus erythematosus disease activity index(SLEDAI)score was ≥8 and with poor first-line treatment were collected, and their clinical manifestations, treatment process, SLEDAI score, complement C3, complement C4, anti-dsDNA antibody titer, and proteinuria relief were analyzed retrospectively.Results:Before treatment with belimumab, the SLEDAI score was 8 in 3 cases, 10 in 5 cases, 12 in 2 cases and 16 in 2 cases.Theurine protein was positive in 6 cases.The anti-dsDNA antibody titer was higher than normal value in 8 cases.The complement C3 decreased in 8 cases and the complement C4 decreased in 6 cases.The SLEDAI scores and the anti-dsDNA antibody of 12 children and 24-hour urine protein quantification of 6 children with positive urine protein began to decrease within 4 weeks after treatment with belimumab.Anti-dsDNA antibody decreased to normal in 12th week and 24 h urine protein decreased to normal in 16th week.The levels of complement C3 and C4 began to rise within 4 weeks, complement C3 returned to normal within 24 weeks, and complement C4 returned to normal within 28 weeks.Conclusion:For LN children with poor response to first-line therapy or persistent disease activity, the addition of belimumab resulted in increased complement, decreased disease activity index and anti-dsDNA antibody titer, and effective relief of proteinuria.The application of belimumab has a certain effect on active LN children with poor response to first-line therapy, which is worthy of clinical promotion.

Case report of SAPHO syndrome in children / 中华实用儿科临床杂志

Retrospective analysis.To report a child with synovitis, acne, pustulosis, hyperostosis, osteitis (SAPHO) syndrome admitted to the Department of Pediatric Nephrology and Rheumatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University in March 2020.A 14-year-old boy presented to hospital due to acne for more than 2 months, fever for more than 50 days, and hip pain for 2 weeks.The patient was diagnosed and treated as acne combined with infection and arthritis in other hospitals for more than 2 months without remission.He was initially diagnosed as juvenile idiopathic arthritis, acne combined with infection on admission.The disease was aggravated even after medications of anti-infection and anti-inflammatory drugs.After multidisciplinary consultation, the patient was diagnosed as SAPHO syndrome.The patient recovered from medications of Celecoxib, Sulfasalazine, recombinant human tumor necrosis factor receptorⅡ: IgG Fc fusion protein and other drugs.SAPHO syndrome is rare, especially in children, which is easily misdiagnosed.This case report suggested that SAPHO should be considered in children with acne, especially those combined with arthritis.

Clinical analysis of hyperphosphatemia in children with steroid-sensitive nephrotic syndrome / 中国小儿急救医学

Objective:To analyze the relationship between hyperphosphatemia and steroid-sensitive nephrotic syndrome(SSNS)in children.Methods:A retrospective study was carried out in 61 children with SSNS at Department of Paediatric Nephrology and Rheumatism and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University from January 2017 to December 2018.The changes of serum phosphorus levels during the active and remission stages were observed, and the correlation between serum phosphorus level and serum albumin, blood lipid, renal function, blood calcium, blood phosphorus, urine protein and other indicators were analyzed.Results:Serum phosphorus level of 61 children with SSNS was 1.79(1.65-1.91)mmol/L in the active phase of the disease, of which 33 patients(54.1%)had hyperphosphatemia, while serum phosphorus level was 1.64(1.46-1.79)mmol/L after the complete remission, and 15(24.6%)patients had hyperphosphatemia.Serum phosphorus level was positively correlated with ratio of urine protein/creatinine, serum lipoprotein A and calcium-phosphorus product( r=0.239, P<0.05; r=0.188, P<0.05; r=0.623, P<0.05), and negatively correlated with levels of serum albumin and serum calcium( r=-0.201, P<0.05; r=-0.195, P<0.05). Conclusion:The morbidity of hyperphosphatemia in children with SSNS is quite high during the active stage of the disease.The disorder of blood phosphorus significantly improve with remission of the disease.

Clinical analysis of nine cases of childhood malignancy who initially present with arthritis / 中国小儿急救医学

Objective:To explore the clinical characteristics of malignant tumors with arthritis as the first symptom in children, so as to strengthen the early recognition of juvenile idiopathic arthritis(JIA) and avoid misdiagnosis and treatment.Methods:Nine cases of children with malignant tumor with arthritis as the first symptom were collected from February 2015 to August 2019 in our hospital.The clinical manifestations, laboratory and imaging features of nine cases were analyzed retrospectively.Results:There were nine children, including five males and four females, with an average age of 6.2 years and an average course of 61.4 days.There were seven cases of acute lymphoblastic leukemia(ALL), one case of neuroblastoma(NB) and one case of anaplastic large cell lymphoma(ALCL). Joint symptoms: polyarthritis in four cases and oligoarthritis in five cases.Eight cases had extraarticular symptoms.Nine cases had elevated inflammatory indexes, six cases had mild abnormal blood routine examination, one case was positive for HLA-B27, and other rheumatoid factor, anti CCP antibody, RA33 antibody and antinuclear antibodies spectrum were negative.Bone destruction was found in five cases.There was no significant difference in clinical manifestations and examinations between nine cases of children with malignant tumor and JIA.Conclusion:Arthritis can be the first manifestation of malignant tumor in children.However, JIA lacks specific diagnostic indicators.In clinical practice, malignant tumors with the first manifestation of arthritis can be regarded as JIA and treatment will be delayed.Clinicians need to raise awareness.

The association between postmenopausal osteoporosis and APC gene polymorphism was analyzed based on quantitative CT measurement of lumbar vertebral bone density / 中华内分泌外科杂志

Objective:To investigate the relation of two SNPs of adenomatous polyposis coli (APC) gene E1317Q (RS1801166, G > C) and D1822V (RS45952, A > T) polymorphism with osteoporosis and bone metabolism in postmenopausal women.Methods:A total of 374 postmenopausal women who underwent routine physical examination in the Second Affiliated Hospital of Zhejiang University School of Medicine from Mar. 2019 to Mar. 2021 were selected as subjects, and divided into normal bone mass group (103 cases) , osteoparrosia group (114 cases) and osteoporosis group (157 cases) . Bone mineral density was measured by a 128-slice spiral CT machine manufactured by Siemens. Clinical and bone metabolic indicators were recorded. Two SNPs were genotyped by capillary electrophoresis and fragment analysis (SNaPshot) . The relative expression level of APC gene mRNA was measured in quantitative real-time fluorescence quantitative polymerase chain reaction system.Results:There were significant differences in genotype and allele frequency distribution of APC rs1801166 and RS45952 among the three groups (all P<0.05) . For rs1801166 site, paircomparison results showed that the genotype distribution of the osteoporosis group was significantly different from that of the normal bone mass group and the decreased bone mass group ( P<0.05) . Allele frequency was significantly different between the two groups ( P<0.05) . For rs45952 site, pairwise comparison showed that the genotype distribution and allele frequency were significantly different between the osteoporosis group and the normal bone mass group ( P<0.05) . There were statistically significant differences in serum calcium, serum phosphorus, alkaline phosphatase, 25-hydroxyvitamin and bone mineral density between wild type and mutant type at rs1801166 (all P<0.05) . There were statistically significant differences in serum calcium, alkaline phosphatase, 25-hydroxyvitamin and bone mineral density between wild type and mutant at RS45952 site (all P<0.05) . There was statistical significance in the mRNA relative expression of APC gene in the normal bone mass group, the bone mass reduction group and the osteoporosis group ( P<0.05) . The mRNA relative expression level of APC gene in wild type at rs1801166 and RS45952 sites was significantly higher than that in mutant type (all P<0.05) . Conclusion:APC gene polymorphism is significantly correlated with osteoporosis and bone metabolism in postmenopausal women, and may affect the expression level of APC gene.

Research progress of acute post streptococcal glomerulonephritis in pediatrics / 中国小儿急救医学

Acute post streptococcal glomerulonephritis is one of the most common causes of acute post-infectious glomerulonephritis in developing countries.Now, the specific pathogenesis of the disease is unclear, the clinical manifestations are diverse, and the treatment of this disease is symptomatic support therapy.The study of the pathogenesis is helpful to improve the prevention and treatment of ASPGN.In this review, we focused on the pathogenesis, diagnosis and treatment of acute streptococcal glomerulonephritis in pediatrics.

Clinical analysis and follow-up of 16 children with methylmalonic aciduria combined with renal injury / 中国小儿急救医学

Objective:To analyze the clinical characteristics and prognosis of children diagnosed as methylmalonic aciduria(MMA) with renal injury.Methods:Sixteen patients diagnosed as MMA with renal injury hospitalized in Shandong Provincial Hospital Affiliated to Shandong University from January 2010 to August 2019 were included.The clinical data and prognosis of the patients were summarized and analyzed retrospectively.Results:Sixteen cases were diagnosed as MMA from 1 days to 7 years old(median 6 months). Renal involvement of 16 patients included isolated hematuria(5 cases), isolated proteinuria(1 cases), hematuria and proteinuria(6 cases), nephritis nephrotic syndrome(2 cases), hemolytic uremic syndrome(2 cases), among them 4 cases accompanied by renal function injury.MMACHC mutation was detected in 2 patients diagnosed as MMA CblC type.One child refused to be treated with vitamin B 12(lost to follow-up after discharge). Among the remaining 15 cases, 5 cases died of multiple organ failure, 5 cases abandoned, 5 cases were saved and the urine routine returned to normal after 1-4 months. Conclusion:The manifestations of MMA combined with renal injury are complicated.Early diagnosis and regular treatment are very important to control the disease and improve the prognosis.

A multicenter study of reference intervals for 15 laboratory parameters in Chinese children / 中华儿科杂志

Objective@#To establish comprehensive laboratory reference intervals for Chinese children.@*Methods@#This was a cross-sectional multicenter study. From June 2013 to December 2014, eligible healthy children aged from 6-month to 17-year were enrolled from 20 medical centers with informed consent. They were assessed by physical examination, questionnaire survey and abdominal ultrasound for eligibility. Fasting blood samples were collected and delivered to central laboratory. Measurements of 15 clinical laboratory parameters were performed, including estradiol (E2), testosterone(T), luteinizing hormone(LH), follicle-stimulating hormone(FSH), alanine transaminase(ALT), serum creatinine(Scr), cystatin C, immunoglobulin A(IgA), immunoglobulin G(IgG), immunoglobulin M(IgM), complement (C3, C4), alkaline phosphatase(ALP), uric acid(UA) and creatine kinase(CK). Reference intervals were established according to central 95% confidence intervals for reference population, stratified by age and sex.@*Results@#In total, 2 259 children were enrolled. Finally, 1 648 children were eligible for this study, including 830 boys and 818 girls, at a mean age of 7.4 years. Age- and sex- specific reference intervals have been established for the parameters. Reference intervals of sex hormones increased gradually with age. Concentrations of ALT, cystatin C, ALP and CK were higher in children under 2 years old. Serum levels of sex hormones, creatinine, immunoglobin, CK, ALP and urea increased rapidly in adolescence, with significant sex difference. In addition, reference intervals were variable depending on assay methods. Concentrations of ALT detected by reagents with pyridoxal 5'-phosphate(PLP) were higher than those detected by reagents without PLP. Compared with enzymatic method, Jaffe assay always got higher results of serum creatinine, especially in children younger than 9 years old.@*Conclusion@#This study established age- and sex- specific reference intervals, for 15 clinical laboratory parameters based on defined healthy children.

New progress in the study of IL-17 in renal diseases / 中国医师杂志

Interleukin (IL)17 is a pro-inflammatory cytokine produced by the activated CD4 + T cell subsets (Th17).It can regulate the function of cells involving in the pathogenesis of inflammation,autoimmune,neoplastic disease.The IL-17 family consists of six family members,IL-17A,IL-17B,IL-17C,IL-17D,IL-17E and IL-17F,respectively.The IL-17 receptor family consists of five members,IL-17RA,IL-17RB,IL-17RC,IL-17RD and IL-17RE,respectively.This review summarizes the characteristics and functions of the IL-17 family,the IL-17 receptor family,and the Th17/IL-17 axis.It also reviews the research about IL-17 in various kidney diseases.

Analysis of clinical and genetic features of one family with paroxysmal kinesigenic dyskinesia / 中国神经精神疾病杂志

Objective To study the clinical and genetic features of familial paroxysmal kinesigenic dyskinesia (PKD). Methods The clinical information of 14 family members in one pedigree, including 2 patients (one treated in hos?pital, the other not treated) were analyzed and the response to treatment and prediction were followed up. DNA was ex?tracted from peripheral blood samples, and then screened for PRRT2 mutations. Results There were two male patients in the pure PKD pedigree, Prevalence rate was 14.3%,One of the PKD patients showed good response to carbamazepine as well as lamotrigine whereas other patients recovered without treatment. We detected a nonsense mutation c.797G>A (p.266R>Q) in PRRT2 gene in three family members. One affected member harboring PRRT2 mutation resulted from the incomplete penetrance of the disease,PKD and polycystic kidney disease coexist in the pedigree which showed autoso?mal dominant inheritance with incomplete penetrance and anticipation. Conclusions The curative effect of antiepileptic drugs to purely familial PKD is related to mutations and clinical features;Treatments should be decided based upon clini?cal features and mutations.

Interventional treatment of post-biopsy renal artery pseudoaneurysm in a child: case report and literature review / 中华儿科杂志

<p><b>OBJECTIVE</b>To investigate the characteristics,diagnosis and therapy of post-biopsy renal artery pseudoaneurysm in children and to study the clinical value of arterial embolization for traumatic renal hemorrhage when conservative treatment failed.</p><p><b>METHOD</b>Data were compiled from medical records of a child in whom renal artery pseudoaneurysm occurred after biopsy in the Provincial Hospital Affiliated to Shandong University , and the related literature was reviewed to analyze the diagnosis and treatment of such pseudoaneurysm.</p><p><b>RESULT</b>A 13-year-old boy had gross hematuria, aggravated dysuria and decreased hemoglobin 10 days after percutaneous renal biopsy. Hb decreased from 110 g/L on the first day after admission to 92 g/L on the 4th day, 83 g/L on the 7th day and the minimum to 74 g/L at the 8th day after admission. Ultrasound showed solid echogenic mass in the right renal pelvis as well as the bladder. Color Doppler ultrasound shows the red and blue rotation of blood flow in the polar capsule under the right kidney. Contrast-enhanced CT in the arterial phase showed a 0.5 cm sized renal mass with a strongly enhanced dot in the lower pole of the right kidney, suggesting a renal artery pseudoaneurysm. Haemostatic, supplement of red blood cells and blood volume and other integrative treatment of hematuria were applied for seven days, but his gross hematuria continued to be worsened. He was diagnosed as pseudoaneurysm by digital subtraction angiography (DSA) on the 19th day after renal biopsy. Superselective renal artery embolization using micro-coils and gelatin sponge particles was performed, and the blood clots were cleaned under cystoscope. Macro-haematuria and dysuria disappeared after the interventional treatment. Retrieval of reports on post-biopsy renal artery pseudoaneurysm in children by using "pseudoaneurysm, child" as the search term showed report of one case from the Chinese CNKI database and 3 cases from the PubMed database. The underlying disease was Henoch-Schonlein purpura nephritis in 3 cases and Sneedon syndrome in 1 case; clinical manifestation of gross haematuria was present in 4 cases, lumbago or pain at the site of the puncture in 2 cases, dysuria in 1 case, and fever in 2 cases.</p><p><b>CONCLUSION</b>The post-biopsy renal artery pseudoaneurysm in children is often manifested as gross hematuria, lumbago, pain at the site of the puncture, fever and dysuria, DSA can be used for definite diagnosis and the interventional treatment is effective.</p>

The effects of periodontal treatment on the levels of serum lipids and lipoprotein associated phospholipase A2 in patients with chronic periodontitis and metabolic syndrome / 实用口腔医学杂志

Objective:To study the effects of periodontal treatment on the levels of serum lipids and lipoprotein associated phospho-lipase A2(LP-PLA2)in patients with chronic periodontitis(CP)and metabolic syndrome(MS).Methods:52 cases of CP with MS were included,the blood lipid levels,clinical periodontal indexes and white blood cell count(WBC)were detected before and 3 months after treatment.Results:Before and after periodontal therapy the levels of AL(mm)were 5.02 ±0.68 and 3.61 ±0.43(P<0.01),PD(mm)4.07 ±0.46 and 2.52 ±0.39(P<0.01),BOP positive loci(%)92.13 ±6.98 and 37.41 ±8.19(P<0.01), PLI 1.38 ±0.29 and 0.89 ±0.27(P<0.05),TG(mmol/L)1.99 ±0.42 and 1.45 ±0.32(P<0.01)and TC(mmol/L)6.11 ± 0.38 and 5.17 ±0.41(P<0.01),HDL(mmol/L)1.06 ±0.22 and 1.41 ±0.19(P<0.05),respectively.Before and after treat-ment WBCs(×103/L)were 6.03 ±0.42 and 5.52 ±0.37(P<0.01),serum LP-PLA2(mg/L)31.02 ±9.81 and 23.89 ±14.15 (P<0.01),respectively.Conclusion:Periodontal therapy can improve the blood lipid levels in patients with CP and MS.

Construction and Application of TCM Medical Treatment and Clinical Scientific Research Information Sharing System Based on Hepatitis B-associated Liver Failure (Gan-Wen) / 世界科学技术-中医药现代化

The China Academy of Chinese Medical Sciences (CACMS) designed and applied the Traditional Chi-nese Medicine (TCM) Medical Treatment and Clinical Scientific Research Information Sharing System based on Hepatitis B-associated Liver Failure (Gan-Wen ) . The network and hardware environment were built . And the electronic medical record system which fits for TCM treatment and research was also established. The system is interfaced to various clinic information systems. Standard TCM terminology database and medical record templates were built. The TCM clinical multidimensional data warehouse was constructed to achieve data mining and im-prove TCM research ability and efficiency .

Biological healing mechanism of magnetic porous Ca3 (PO4) 2: an experimental study / 中华创伤杂志

Objective To study the causes and theoretical basis for good bone healing ability of magnetic Porous Ca3 (PO4) 2 ( MPTCP). Methods Seven MPTCP specimens with size of 2 cm × 1 cm × 0.5 cm were placed in the material physical system for detecting 42 times and the mean detection value was used to measure the MPTCP curve. The attachment 16451B of impedance spectrometer HP RLC was employed to measure dielectric spectroscopy and dielectric spectroscopy of MPTCP. Four-wire method was used to measure the impedance of MPTCP. Results The magnetic intensity changed rapidly when magnetic field was in a range of-10,000-10,000 Oe. The peak of dielectric spectroscopy and impedance of magnetic bioceramics was in the range of 103-104 Hz. When the external electromagnetic wave of frequency was ≤ 1 000 Hz, electrical impedance of MPTCP was large;while when the electromagnetic wave frequency was≥1 000 Hz, the impedance was relatively small and stable. Conclusion The environmental magnetic fields may change the magnetic and electric behavior of MPTCP and promote the biological healing, which may be the cause for the good bone healing ability of MPTCP.

Effects of variant rs346473 in ARHGAP24 gene on disease progression of HBV infection in han Chinese population / 华中科技大学学报（医学）（英德文版）

Host genetic, environmental and viral factors are classified as three categories that determine clinical outcomes of hepatitis B virus (HBV) infection. The objective of this study was to detect the associations between polymorphisms rs346473 and rs346482 in Rho GTPase-activating protein 24 (ARHGAP24) gene and disease progression of HBV infection in Han Chinese population. These two SNPs were found by our DNA pooling using Affymetrix Genome-Wide Human Mapping SNP6.0 Array in HBV carriers, and verified by using TaqMan 7900HT Sequence Detection System with 758 progressed HBV carriers versus 300 asymptomatic HBV carriers (AsC) in a discovery phase and 971 progressed HBV carriers versus 328 AsC in a replication phase. Multivariable logistic regression revealed that individuals with genotype TT at variant rs346473 displayed remarkable correlations with disease progression of HBV infection both in the discovery phase (OR, 2.693; 95% CI, 1.928-3.760; P=6.2×10(-9); additive model) and the replication phase (OR, 1.490; 95% CI, 1.104-2.012; P=9.0×10(-3); additive model). These two SNPs were in strong linkage disequilibrium with D'=0.99 and r (2)=0.951, and haplotype TT disclosed an increased susceptibility to HBV progression (OR, 1.980; 95% CI, 1.538-2.545; P=8.1×10(-8)). These findings suggest that polymorphism rs346473 in the ARHGAP24 gene might be a part of the genetic variants underlying the susceptibility of HBV carriers to disease progression.

Observation of therapeutic efficacy of Ambrocol aral solution for lower respiratory tract infection in children / 中国基层医药

Objective To observe the effect and adverse reaction of ambrocol oral solution on treatment of children with lower respiratory tract infection. Methods 160 children hospitalized with lower respiratory tract infection in children according to odd and even numbers were randomly divided into two groups on the basis of conventional therapy,the treatment group were given oral ambrocol oral solution,the control group treated with oral ambroxol hydrochloride syrup.The clinical efficacy and adverse reaction were observed. Results 62 cases were excellent,effective in12 cases,not effective in 6 cases,total effective rate 92.5 %,and the control group 50 cases,15 cases,81.3 % compared to the total effective rate between the two groups was statistically significant(x2=4.44,P＜0.05＝; treatment group cough disappearance time(4.5 ± 1.4)d,expectoration difficulties disappear time(3.1 ± 1.2)d,wheeze disappearance time(2.9 ± 1.2)d,and the control group(5.4 ± 1.5)d,(3.5 ± 1.2)d,(3.7 ± 1.4)d phase by comparison,the differences were statistically significant(t=3.930,2.105,7.767,all P <0.05). Conclusion Ambrocol oral solution is appropriate for children of all ages expectorant cough medicine,high efficacy ,safety ,and can improve the clinical effects of lower respiratory tract infection.

Effect of additives on the microstructure of machinable bioglass-ceramic / 中国组织工程研究

BACKGROUND:Machinable bioglass-ceramics became a new inorganic biomaterial; moreover, strength, toughness and machinability are significantly studied.OBJECTIVE: To observe the effect of ZnO, Fe2O3 and ZrO2 additives on the microstructure and properties of machinable bioglass-ceramic in K2O-MgO-CaO-SiO2-P2O5-F system.DESIGN: Observational contrast study.SETTING: Department of Orthopaedics, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: The experiment was carried out in the Laboratory of Materials, Wuhan University of Technology from October 2003 to April 2004. K2O-MgO-CaO-SiO2-P2Os-F system, ZnO, Fe2O3 and ZrO2 additives, D/Max-ⅢA X-ray diffractometer (Japan), JSM-5610LV scanning electron microscope (SEM), HVS-1000 microhardnessmeter and 3257-35 magnetic testing device (Japan) were used in this study.METHODS: In the glass-ceramic of K2O-MgO-CaO-SiO2-P2O5-F system, three kinds of additives ZnO, ZnO-Fe2O3 and ZnO-Fe2O3-ZrO2 were added respectively. These three kinds of ceramics were prepared by being melted at 1 400 ℃ for 1 hour. After fire, crystal temperature was set based on differential thermal analysis curve. ① Physical properties: Microhardness was determined with microhardnessmeter (HVS-1000). The flexural strength and fracture toughness were established measured with ceramic mechanical test system (MTS) method and single edge notched beam (SENB) method, respectively. Saturation magnetic moment and Curie temperature were determined with 3257-35 magnetism test machine made in Japan. Holing method was used to measure machinability. ② Test of crystalline phase and microstructure: Crystalline phase analysis was carried out with X-ray diffraction (XRD: D/Max-ⅢA) and microstructure analysis were conducted on an etched fracture surface using SEM (JSM-5610LV,Japan). MAIN OUTCOME MEASURES: Effects of ZnO, Fe2O3 and ZrO2 additives on physical properties, mineral composition and crystalline phase.RESULTS: ① The addition of ZnO to the glass-ceramic in K2O-MgO-CaO-SiO2-P2O5-F system was beneficial to crystal growth, increasing aspect ratio of crystal, decreasing microhardness and improving toughness and machinability. ② The addition of ZnO-Fe2O3 to the glass-ceramic in K2O-MgO-CaO-SiO2-P2O5-F system can form minority Mg-Zn ferrite with magnetism. The main crystalline phase present in the glass- ceramic was diopside with small size and hardness was improved, which led to worse machinability. ③ Because of the addition of ZnO-Fe2O3-ZrO2, magnesia fluormica and fluorapatite became the main crystalline phases, and the minor phases including Mg-Zn ferrite, t-ZrO2 and m-ZrO2, etc.also presented in the glass-ceramic. This material possessed high strength and toughness, good machinability and magnetism, which could stimulate formation of new bone, and was good substitute of bone restorations. Bioassay and in vitro test indicated that the glass-ceramic in K2O-MgO-CaO-SiO2-P2O5-F system was bioactive and biocompatible. CONCLUSION: ZnO, Fe2O3 and ZrO2 additives play a significant role in changing crystalline structure, enhancing strength,toughness and machinability of machinable bioglass-ceramic and generating magnetism.

Effect of tongjingning granule on primary dysmenorrhea / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the mechanisms of tongjingning granule (TJN) on primary dysmenorrhea (PD).</p><p><b>METHODS</b>One hundred and twenty PD patients were treated with TJN, and 40 patients treated with aspirin as controls. The levels of serum estrogen (E2) and progestin (P) content in the luteal metaphase and anaphase (MI/AI) as well as endothelin (ET) and calcitonin gene related peptide (CGRP) in the metaphase and menstrual stage before and after treatment in partial patients were determined.</p><p><b>RESULTS</b>The therapeutic effect , and the curative markedly-effective rate and improvement rate of main accompanied symptoms of severe and moderate PD patients in the treated group were superior than those in the control group (P < 0.01). The level of E2 and ET were significantly decreased, and the content of P and CGRP were significantly increased in the treated group after treatment respectively (P < 0.01).</p><p><b>CONCLUSION</b>JTN not only can regulate ovarian hormone, ET and CGRP, but shows advantages in adjusting spirits and emotions, improving homeostasis and consolidating the therapeutic effect.</p>

STUDY ON ADRIAMYCIN-POROUS TRICALCIUM PHOSPHATE CERAMIC DRUG DELIVERY SYSTEM AND ITS DRUG RELEASE TEST IN VIVO / 中国修复重建外科杂志

Objective　To manufacture adriamycin-porous tricalcium phosphate (A-PTCP) ceramic drug delivery system (DDS)as a possible method for bone defect treatment after bone tumor operation. Methods　A-PTCP DDS was made from putting adriamycin into PTCP. Thirty rabbits were divided randomly into group A(24 rabbits) and group B(6 rabbits). A-PTCP was implanted in the greater trochanter of the right femur in group A. Adriamycin were injected into veins in group B. Muscle around A-PTCP and plasma were taken out at different period. Adriamycin concentrations in muscle and plasma were measured by high performance liquid chromatography (HPLC). Results　A-PTCP could gradually release adriamycin over 10 weeks. Adriamycin concentrations in the muscle were higher than that in plasma. Conclusion　A-PTCP may be a new method for repairing bone defects after bone tumor operation.

Clinical and Pathological Analysis of Acute Renal Failure in Children / 实用儿科临床杂志

Objective To study the etiology, clinical and pathological characteristics, therapy and prognosis of acute renal failure (ARF). Methods The literature of 80 treated children with ARF from 1988. 1 to 2003.10 was reviewed. Results Sixty three cases (78.8%)suffered from mtrarenal ARF;47 glomerulonephritls (58. 8%); 15(18. 8%)prerenal ARF;2(2 5%)postrenal obstructive ARF. Twenty three cases with renal biopsy,9(39. 1%)MsPGN;4 (17. 4% ) MPGN; 4 (17. 4%) crescentic glomerulonephntis; 3 (13% ) endocapillary glomerulonephritls;2(8 7% )proliferative and sclerosing glomerulonephritis; 1(1. 3% )minimal changes. According to the different etiologic and pathological characteristics,different therapies were applied,infusion,oral prednisone ,and intravenous pulses of methylprednisone and cyclosphosphamide ,dialysis, or diuretic therapy and lowering blood. Thirty one (38. 8% )cases recov-ered;27(33 8% )cases improved; 10( 12.5% ) cases gave up treatments;21 (26 3% )died. The mortality of ARF from 1996 to 2003 was significantly lower than that from 1988 to 1995(x2 = 7.85 P