Relationship between expression of miR-203 and FGF-2 in children with hemangiomas and its significance / 国际儿科学杂志

Objective:To analyze the expression levels of microRNA-203(miR-203)and fibroblast growth factor-2(FGF-2)in hemangiomas of infancy(HOI)and to explore their clinical significance.Methods:A total of 55 patients with HOI admitted to our hospital from March 2016 to August 2017 were selected as HOI group and divided into proliferative phase(31 cases)and regressive phase(24 cases), normal tissue specimens were taken as control group(34 cases). Real time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression levels of miR-203 and FGF-2 mRNA in HOI tissues.Immunohistochemistry was used to detect the expression of FGF-2 protein in HOI tissues.Clinical indicators of HOI patients included angiogenin(ANG), vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF), glucocorticoid receptor alpha(GRα), glucocorticoid receptor beta(GRβ). Pearson method was used to compare and analyze the correlation between various indicators in children with HOI group.Logistic multivariate regression analysis was used to analyze the related influencing factors of HOI.Results:Compared with the control group(1.01±0.15), the expression level of miR-203 in HOI group(0.73±0.24)decreased significantly( P<0.05), and the proliferative phase(0.72±0.21)was significantly higher than the regression phase(0.59±0.19)( P<0.05); the expression levels of FGF-2 mRNA and protein in HOI group(2.38±0.74)were significantly higher than those in control group(1.02±0.14)( P<0.05), and the regression phase(2.37±0.79)was significantly higher than the proliferative stage(2.03±0.68)( P<0.05); Pearson analysis showed that miR-203 was negatively correlated with FGF-2, ANG, VEGF and bFGF( P<0.05), while FGF-2 was positively correlated with them( P<0.05); Logistic analysis showed that the expression levels of miR-203 and FGF-2 were both influencing factors of HOI. Conclusion:MiR-203 is low expressed in HOI, while FGF-2 is highly expressed.There is a significant difference in the expression changes of the two in HOI stages and it is important for clinical diagnosis of HOI and treatment.

Comparisonbetweencomplicationsandrecurrenceofdifferentinterventionalmethods inthetreatmentofbenignesophagealstenosisinchildren / 实用放射学杂志

Objective Toinvestigatetheinterventionaltreatmentmethodsofbenignesophagealstenosisinchildren,includingballoondilation, stentimplantationandcontinuousballoondilation.Methods 42casesofesophagealstenosisdiagnosedbybariumexaminationinour hospitalfromJanuary2010toJune2017.Accordingtothetreatmentmethods,theyweredividedintoballoondilationgroup(13cases),stent implantationgroup (11cases),continuousballoondilationgroup (18cases),andthecomplicationsandrecurrenceratesofthree methodswerecompared.Results Balloonorstentsweresuccessfullyplacedorremovedinallcases,andtheclinicalsymptomswere significantlyimproved.Therewerenoseriouscomplicationssuchasruptureofesophagus,perforationandmassivehemorrhageI.nballoondilation group,restenosisoccurredin10cases3to10monthsafteroperation,withcomplicationsrateof76.9% andrecurrencerateof76.9%. Instentimplantationgroup,sentfallingoffordisplacedoccurredin4casesandrestenosisin4cases,withthecomplicationsrateof 72.7% andrecurrencerateof36.3%.Incontinuousballoondilationgroup,therewere2casesofballoondescendingand4casesof restenosis,withcomplicationsrateof33.3% andrecurrencerateof22.2%.Comparingthecomplication,theballoondilationgroup>thestentimplantationgroup(χ2=0.056,P>0.05),thestentimplantationgroup>thecontinuousballoondilationgroup(χ2=4.243, P<0 .05 ),the balloon dilation group>the continuous balloon dilation group (χ2=5 .743 ,P<0 .05 ).Co m paring the recurrence rate ,the balloondilationgroup>thestentimplantationgroup(χ2=4.033,P<0.05),thestentimplanationgroup>thecontinuousballoondilationgroup (χ2=0.684,P>0.05),theballoondilationgroup>thecontinuousballoondilationgroup(χ2=9.120,P<0.05).Conclusion There aredifferentincidenceofcomplicationsandrecurrenceinthreemethods,andthecontinuousballoondilationissimplerandlesscomplicationsand recurrencethantheothertwomethods.Itcanbethefirstchoiceforinterventionaltreatmentofesophagealstenosis.

Clinical relevant factors of air enema reduction in children with intussusception / 中国医师进修杂志

Objective To discuss the clinical relevant factors of air enema reduction in children with intussusception. Methods The clinical data of 1 223 children with intussusception from January to October 2017 were reviewed retrospectively. The clinical features of air enema reduction failure were analyzed. Results Among the 1 223 children with intussusception, 1 140 children achieved successful reduction by air enema, and 83 children were failed. The reduction rate was 93.2% (1 140/1 223). Among the 83 failed children, 64 cases had bloody stools, 5 cases had no defecation since onset, and 51 cases were depression and lethargy. The course of disease was 6 h to 5 d. The ileocolonic intussusceptions was in 51 cases, ileo-ileo-colon intussusceptions in 15 cases, ileum-ileum intussusceptions in 6 cases, ileo-ileo-colonic-colonic intussusceptions in 3 cases, and ileum-cecum intussusceptions in 8 cases. Among the 51 ileocolonic intussusceptions children, there were 2 cases whose nested head located in the sigmoid, 6 cases in the liver area, 23 cases in the middle of the transverse colon, 15 cases in the middle of the ascending colon, and 5 cases in the ileocecal junction. The length of the intussusceptions was more than 5 cm, with 5 to 10 cm in 51 cases, 11 to 20 cm in 27 cases, and 21 to 30 cm in 5 cases. The diameter of nested head was 4 to 8 cm, with an average of 5 cm. The failed air enema children were treated with operation. Two cases of intestinal duplication, 13 cases of Meckel diverticulum, 2 cases of juvenile polyp, 2 cases of Peutz-Jeghers syndrome were found during operation. Conclusions There has low successful rate of air enema reduction in the children with long duration, much older, poor whole body condition and complex or secondary intussusception. Correct judgment of surgical indications could increase the successful rate of air enema reduction.

The clinical efficacy of ultraselective intravascular embolization combined with local sclerotherapy in the treatment of large hemangioma in children / 实用放射学杂志

Objective To study the clinical efficacy and complications of ultraselective endovascular embolization combined with local sclerotherapy in the treatment of large hemangioma in children,to provide a better choice for the treatment.Methods 85 cases of large hemangioma in our department in the last three years were analyzed retrospectively.Patients were divided into two groups according to the treatment:the simple treatment group (33 cases),ultraselective endovascular embolization,and the combination treatment group (52 cases),ultraselective endovascular embolization combined with local sclerotherapy or local sclerotherapy after endovascular embolization.The total effective rate and the incidence of complications between two groups were compared,and the patients were followed up for 3 months to 1.5 years.Results 85 cases of large hemangioma were successfully treated by simple ultraselective endovascular embolization or combined treatment.A total of 75 cases were treated effectively (complete cure,partial cure,condition improved)and 10 cases were ineffective and no recurrence or tumor enlargement in the follow-up .In the total effective rate,the combination treatment group [96.2% (50/52)]was superior to the simple treatment group [75.8% (25/33)](χ2=6.245,P=0.012 ).There was no significant difference in the complete cure rate between the two groups (χ2=1.561,P=0.212 ).The complication rate in the simple treatment group was slightly higher than that in the combined treatment group:36.4% (12/33)vs 32.7% (17/52)(χ2=0.064,P=0.996).Conclusion The total effective rate of ultraselective endovascular embolization combined with local sclerotherapy in the treatment of large hemangioma in children is better than simple endovascular embolization,and less complications,safely treatment and less trauma.It can be used as the first choice for treatment of large hemangioma.

Analysis of the efficacy of superselective intravascular embolization in the treatment of large hemangioma in children / 中华放射学杂志

Objective To evaluate the clinical efficacy of superselective intravascular embolization in the treatment of large hemangiomas in children,and to provide a reliable basis for the clinical treatment and to predict prognosis.Methods The clinical and imaging data of 85 children with large hemangiomas who underwent superselective angiography and embolization were collected in our hospital from January 2015 to January 2018,and followed up for 3 months to 1.5 years.According to the DSA angiography of the hemangioma [artery origin,artery number,arterio-venous fistula(AVF)],and combined with age,tumor size and the selection and compatibility of embolization materials.Using the chi-square test to analyze these indicators.Results Analysis of the clinical efficacy indicators,was shown as the following:(1) Artery origin:The total effectiveness rate of single and mixed sources was 95.3％ (61/64),66.7％ (14/21) respectively.The difference was statistically significant (x2=0.688,P＜0.05).(2) Feeding artery number:The total effectiveness rate of 1 branch,2 branches and ≥3 branches was (3/3),(25/27)and (47/55) respectively.The difference was statistically significant (x2=1.944,P＜0.05).(3) Presence of fistula(AVF):The total effectiveness rate of AVF and without AVF was 36.4％ (4/11) and (95.9％,71/74) respectively.The difference was statistically significant (x2=0.806,P＜0.05).Other influencing factors including:(1)Age:the recurrence rate of patients≤5 months and＞5 months was 33.3％ (6/18) and 20.9％ (14/67)respectively.There was no significant difference (x2=1.220,P＞0.05);(2)Tumor size:The recurrence rate of φ≤80 mm and φ＞80 mm was 25.5％(13/51)and 32.4％(11/34) respectively.There was no significant difference (x2=0.474,P＞0.05).(3) Selection and compatibility of embolization materials:The recurrence rate of Lipiodol + bleomycin and gelatin sponge +bleomycin was 27.0％ (10/37) and 31.3％ (15/48) respectively.There was no significant difference (x2=0.179,P＞0.05).Conclusions The clinical efficacy of superselective intravascular embolization in the treatment of large hemangioma in children was influenced by:artery origin(mixed sources),artery number (≥3 branches),AVF,age tumor size,selection and compatibility of embolic materials.

The interventional treatment of complex lymphatic malformation in children / 中华放射学杂志

Objective To explore the interventional treatment method for complex and refractory lymphatic malformation in children.Methods The clinical data of 78 cases with complex and refractory lymphatic malformation during January 2013 to January 2016 in our department were retrospectively analyzed.The lesions involved the neck in 28 cases,maxillofacial regions in 19 cases,the chest and armpit in 8 cases,the limb in 7 cases,the pelvic cavity and retroperitoneal space in 6 cases,the superior mediastinum in 3 cases,the hypogloeeis in 3 cases and scrotum or perineum region in 4 cases.All the children underwent ultrasound or MRI imaging studies preoperatively.The interventional procedures included:(1) Percutaneous puncture of the LM for sclerotherapy.The lesions were punctured with 20 G needle under the guidance of DSA or ultrasound and the correct positions were confirmed with angiography.The liquid of the lesions was extracted as far as possible.The dosage of sclerosing agents was adjusted according to the size of lesion.The dose of Laurolacrogol injection was 1/10—1/5 of the amount of the liquid in the lesions and the maximum of Laurolacrogol foam was ≤8 ml(20 mg).The dose of Pingyangmycin was ≤ 8 mg.(2) The drainage catheter placement and sclerotherapy.Percutaneous catheter drainage under ultrasound guidance or by surgery was conducted.The liquid in the lesions was drained by retaining the catheter for 1-4 weeks,and sclerotherapy was applied for several sessions during this period.(3) Treatment for one time a week,2 times a session.The interval of every two sessions was 4 weeks.The sclerosing agents included:Laurolacrogol Injection or Pingyangmycin for the lesions with high tension,Laurolacrogol foam for the lesions with low tension,Pingyangmycin for the microcystic lesions.Statistical analysis was conducted using SPSS20.0 software.Results A total of 208 sessions of sclerotherapy for 78 LM patients were performed and average session was (3.0±0.8).Nine patients used Lauromacrogol foam,23 patients used Lauromacrogol Injection,39 patients used pingyangmycin,while combined treatment was conducted in 7 patients.Laurolacrogol injection was used in 20 cases,Pingyangmycin in 29 cases and combination therapy in 5 cases for the high tension lesions in the neck,maxillofacial,chest and armpit.The low tension lesions of pelvic cavity and retroperitoneal space in 6 cases and of superior mediastinum in 3 cases were placed with draining catheters,and treated with sclerotherapy with Laurolacrogol foam.Three cases with hypogloeeis LM was neonates,who were treated with Laurolacrogol injection.The lesions on the limb and scrotum or perineum region were almost microcystic,were treated with Pingyangmycin in 9 cases and with combination therapy in 2 cases.The total curative rate was 97.4％ (76/78),total effective rate was 100％ (78/78).Imaging examinations showed that the cavities were closed or only a small amount of residual sclerotic lesions were present.Clinical examinations showed that the surface masses almost disappeared.The follow up period was 6 months to 2 years.There were no serious complication and adverse reactions occurred.Conclusions Interventional treatment is a safe,effective,and minimally invasive treatment for the complex and refractory lymphatic malformation.In order to receive the best treatment effect,we should use targeted therapy for different region and type of LM.

Changes in ambulatory electrocardiographic findings after oral administration of low-dose propranolol in infants with hemangioma / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the effects of oral administration of low-dose propranolol on heart rate variability (HRV), acceleration capacity (AC), deceleration capacity (DC), and cardiac conduction in the treatment of infantile hemangioma.</p><p><b>METHODS</b>A total of 118 infants with hemangioma (≤1 year) were enrolled, and 24-hour ambulatory electrocardiography was performed before oral administration of low-dose propranolol and after one month of administration. The changes in time-domain indices [standard deviation of all normal sinus RR intervals (SDNN), standard deviation of all mean 5-minute RR intervals (SDANN), root mean squared successive difference (RMSSD), and percentage of successive normal sinus RR intervals >50 ms (PNN50)] and frequency-domain indices [low frequency (LF) and high frequency (HF)] for HRV, AC, and DC were observed, as well as abnormalities in cardiac conduction and other aspects after administration of propranolol.</p><p><b>RESULTS</b>After administration of propranolol, the infants had significantly increased SDNN, RMSSD, LF, HF, and PNN50 (P<0.01), and significantly reduced AC, mean heart rate (HR) and minimum HR (P<0.01). The 24-hour ambulatory electrocardiographic findings showed a nonsignificantly higher abnormal rate after administration of propranolol.</p><p><b>CONCLUSIONS</b>In the treatment of infantile hemangioma, propranolol can inhibit the activity of sympathetic nerve and block cardiac conduction, but without any serious adverse effect.</p>

Interventional approaches to neonatal Kasabach-Merritt syndrome：method selection and therapeutic effectiveness / 中华放射学杂志

Objective To explore therapeutic effects of different interventional approaches to neonatal Kasabach-Merritt syndrome (K-MS) Methods A retrospective analysis of therapeutic effects of different interventional approaches to K-MS in 12 neonates from May 2010 to August 2014. All the patients received local treatment (n=5), chemoembolization (n=4) and combined local treatment and endovascular chemoembolization (n=3), respectively, depending on size, location and blood supply of K-MS. The K-MS patients with angioma measuring5 cm received local treatment, endovascular chemoembolization and combined local treatment and endovascular chemoembolization, respectively. The patients were categorized as cured, improved and clinical inefficient, respectively, based on therapeutic effect. The criteria for cured patients were: (1) angioma faded completely or mostly; (2) the platelet count stabilized within the normal range; (3)no recurrence for more than six months. The criteria for improved patients were: (1) angioma shrinked significantly; (2) the platelet stabilized within the normal range. The criteria for clinical inefficient patients were: (1) agioma no obviously shrinked, the platelet count has no improvement over 2 months after interventional approaches. Results All the 12 patients with K-MS were cured and had no complication. Of the 12 patients, 5 patients received local treatment had decreased localized tension and ecchymosis of hemangioma at the first day of therapy, were cured after 2 to 7 courses of therapy. Of the 4 patients received endovascular chemoembolization, 3 were cured after one course of treatment, 1 had recurrent disease one month later after one course of therapy and were cured after the second course of treatment.One had recurrent disease a month later after one course of treatment and were cured after the second course of treatment. Three received combined local treatment and endovascular chemoembolization, angioma shrinked significantly and increased platelet count after the first course of treatment, and 2 to 3 courses local treatment were combined, hemangioma faded and platelet count stabilized within the normal range after 2 to 3 courses of local treatment. Conclusions Interventional therapy is the most effective treatment of neonatal K-MS.There are different interventional approaches to optimize treatment of neonatal K-MS depending on sizes, location and blood supply of K-MS.

Modified continuous balloon dilatation substituting stenting in the treatment of benign esophageal stenosis in children / 中华放射学杂志

Objective To explore the feasibility of using the modified continuous balloon dilatation substituting the traditional membrane-covered stent in the treatment of benign esophageal stenosis in children.Methods A retrospective analysis of the clinical data of 25 cases with esophageal stenosis from January,2012 to February,2014 was conducted.Preoperative esophageal stenosis was confirmed by angiography,and the expansion of the stenosis segment was examined after 2-4 weeks postoperatively.According to the age and the location of the esophageal stricture,25 cases were classified into two groups.Twelve cases of group A without obvious contraindications were treated by continuous balloon dilatation,while 13 cases of group B with age of less than 3 years or upper esophageal stenosis,retrievable stent implantation was performed.All cases were followed up from 6 months to 2 years.Clinical symptoms,swallowing fluency and the degree of esophageal stricture were followed up at 1,3,and 6 months postoperatively,and the effect of treatment was evaluated.Results Two groups of patients were successfully treated,and the stents were removed successfully after 2-4 weeks.The average diameter of the stenosiswas significantly increased after treatment,swallowing was improved obviously,with no perforation,hematemesis and melena and other serious complications.The balloon moved down happened in one case,which was adjusted under perspective observation.One case in group A underwent colonic interposition for esophageal replacement because of recurrence of restenosis.The balloon dilatation was effective in 10 cases.In Group B,5 patients had stent migration or displacement,3 cases had recurrent esophageal stricture,and the treatment was effective in 5 cases.There was significant difference in complications between the group A and B (x2=5.23,P＜0.05).Conelusions Continuous balloon dilatation is a simple,effective procedure with fewer complications in the treatment of children with benign esophageal stenosis.In addition to stenting,it may be another ideal choice for benign esophageal stenosis in children.

Prevention and treatment of common complications after esophageal retrievable covered stent placement in children / 实用放射学杂志

Objective To explore risk factors,prevention and treatment of common complications of benign esophageal stenosis treated with the retrievable covered stents in children.Methods Fifteen cases,diagnosed as benign esophageal stenosis by barium esophagogram were treated with retrievable covered stents under fluoroscopy,Then the stents were removed after 2-3 weeks.The cases were followed-up and complications were observed.Results The stents were successful implanted in all cases.The diet was improved obviously after operation,and gradually transformed from liquid,semiliquid to normal feeding.At the same time,narrow section gradually expanded from 0.1-0.6 cm to 0.6-1.5 cm.Postoperative follow-up was in 1,2,3 weeks and 3 months.Main complications were stents shift in 5 cases,esophageal restenosis in 2 cases,esophageal-tracheal fistula in 1 case,postoperative dull pain behind sternum and foreign body sensation in 6 cases.Relevant preventions and treatments were performed based on different complications.Conclusion It should not be ignored that complications after covered stent placement are more in children than in adults,preventions and treatments should be taken positively.

Detecting the spectrum of multigene mutations in non-small cell lung cancer by Snapshot assay / 癌症

As molecular targets continue to be identified and more targeted inhibitors are developed for personalized treatment of non-small cell lung cancer (NSCLC), multigene mutation determination will be needed for routine oncology practice and for clinical trials. In this study, we evaluated the sensitivity and specificity of multigene mutation testing by using the Snapshot assay in NSCLC. We retrospectively reviewed a cohort of 110 consecutive NSCLC specimens for which epidermal growth factor receptor (EGFR) mutation testing was performed between November 2011 and December 2011 using Sanger sequencing. Using the Snapshot assay, mutation statuses were detected for EGFR, Kirsten rate sarcoma viral oncogene homolog (KRAS), phosphoinositide-3-kinase catalytic alpha polypeptide (PIK3CA), v-Raf murine sarcoma viral oncogene homolog B1 (BRAF), v-ras neuroblastoma viral oncogene homolog (NRAS), dual specificity mitogen activated protein kinase kinase 1 (MEK1), phosphatase and tensin homolog (PTEN), and human epidermal growth factor receptor 2 (HER2) in patient specimens and cell line DNA. Snapshot data were compared to Sanger sequencing data. Of the 110 samples, 51 (46.4%) harbored at least one mutation. The mutation frequency in adenocarcinoma specimens was 55.6%, and the frequencies of EGFR, KRAS, PIK3CA, PTEN, and MEK1 mutations were 35.5%, 9.1%, 3.6%, 0.9%, and 0.9%, respectively. No mutation was found in the HER2, NRAS, or BRAF genes. Three of the 51 mutant samples harbored double mutations: two PIK3CA mutations coexisted with KRAS or EGFR mutations, and another KRAS mutation coexisted with a PTEN mutation. Among the 110 samples, 47 were surgical specimens, 60 were biopsy specimens, and 3 were cytological specimens; the corresponding mutation frequencies were 51.1%, 41.7%, and 66.7%, respectively (P = 0.532). Compared to Sanger sequencing, Snapshot specificity was 98.4% and sensitivity was 100% (positive predictive value, 97.9%; negative predictive value, 100%). The Snapshot assay is a sensitive and easily customized assay for multigene mutation testing in clinical practice.

Compilation and implementation of web-based PBL teaching plan in pediatrics / 中华医学教育探索杂志

The web-based PBL teaching plan in pediatrics embodies the network learning environment and learning requirements.The basic design unit is a complete real case with certain width and depth,which can stimulate the students' interests of participation and guide students into a predetermined learning area to achieve desired learning objectives.The article dwelled on the compilation and implementation of the teaching plan of children bronchopneumonia.The first part:teachers showed the case in PBL site and proposed the questions.Students made the Powerpoint after studying and discussing in groups by PBL teaching website,QQ group,SMS platform,etc.The second part:students reported and discussed in groups; the teacher provided the new information and put forward the following-up problems; students consulted the documents and materials through the network again; finally teachers made comments on the results.During the teaching,network should be fully utilized and the questions should be proposed progressively at different levels.Teacher should play a guiding role and emphasize the students' autonomous learning.

A single-site retrospective study of pediatric arterial ischemic stroke etiology, clinical presentation, and radiologic features / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Stroke occurs upon obstruction of cerebral blood circulation and is clinically characterized by sudden onset symptoms. Advanced age is the main risk factor of stroke, but cases of pediatric stroke have been rarely reported. This study aimed to determine the etiology, clinical presentation, and radiologic features of neurological deficit for pediatric arterial ischemic stroke (PAIS).</p><p><b>METHODS</b>The medical records of 42 PAIS patients (age range: 9 months to 13 years) treated at Wuhan Children's Hospital between July 2007 and January 2011 were retrospectively reviewed. Infarction location was first determined by craniocerebral computed tomography and magnetic resonance (MR) imaging. The stenotic or occluded main cerebral arteries and/or branches were determined by MR angiography and digital subtraction angiography.</p><p><b>RESULTS</b>The majority of the 42 PAIS cases (66.7%, n = 28) were ≤ 3 years old (vs. >3 years old: 33.3%, n = 14; P<0.05), but the male: female ratio was similar in both groups (P > 0.05). The most frequently reported signs and symptoms for both age groups were limited physical activity followed by convulsions and delirium, but convulsions were more prevalent in children ≤ 3 years-old. Children > 3 years-old mainly experienced the limited physical activity symptoms, including hemiparalysis, aphasia, and ataxia. For all 42 cases, the most frequent etiologies were infections (38.1%, n = 16), iron deficiency anemia (16.7%, n = 7), and moyamoya syndrome (11.9%, n = 5). The predominant infarcts among all cases were middle cerebral artery (63.6%, n = 21) and basal ganglia (64.3%, n = 27).</p><p><b>CONCLUSIONS</b>PAIS occurs more frequently in younger children and this group most frequently presents with convulsion as the initial symptom. The overall etiologies of PAIS may be different from those of adult stroke and the involved regions may be distinguishing features of PAIS or its different forms, but more research is required.</p>

The therapic effect of the combination of endovascular embolization and hormone in Kasabach-Merritt phenomenon / 中华放射学杂志

Objective To explore the therapic effect of the combination of endovascular embolization and clinical hormone in treatment of Kasabach-Merritt phenomenon (KMP),and analyze the advantages.Methods Six cases with KMP from May 2010 to June 2012 were retrospectively analyzed.All the patients underwent large dose hormone shock therapy after admission for 7-10 d.Subsequently,selective endovascular embolization was performed using Seldinger technique under general anesthesia Then,the hormone therapy was continued for 2 weeks after embolization.The platelet count and the effect were recorded.If the area of the tumor reduction is less than 50％,3-4 courses of local hardening treatment was conducted for the residual tumor.The local treatment used multipoint puncturing of the tumor and injection drugs under X-ray fluoroscopic monitoring until the tension of local vascular increased.Results On DSA,the lesions of all the 6 cases showed rich blood supply with a large number of hybrid distribution of tumor blood vessels.The lesions disappeared in 4 cases after 1-2 weeks combination therapy and no recurrence for 0.5-1.0 year follow-up.Two cases whose tumor reduced less than 50％ after combination therapy received local hardening treatment,and faded after 3-4 courses.The number of platelet for all patients kept normal and the spirit of the patients showed great improvement,the bleeding tendency and local soft tissue swelling was in remission.Subcutaneous blood stasis and petechiae disappeared.There were no serious adverse reaction and complications.Conclusion Combination therapy with endovascular embolization and clinical hormone for Kasabach-Merritt phenomenon has a good curative effect.

Study on sperm damage caused by trichloroethylene in male rats / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study in vitro sperm damage caused by trichloroethylene in male rats.</p><p><b>METHODS</b>Sperms of Sprague-Dawley (SD) rats were collected 4 hours after being contaminated by trichloroethylene of 0, 2, 4, 6, 8, and 10 mmol/L in vitro. Giemsa staining was performed to observe the morphological changes of sperms, and flow cytometer was used to detect the changes in mitochondrial membrane potential.</p><p><b>RESULTS</b>The sperm motilities in 6, 8, and 10 mmol/L trichloroethylene groups decreased significantly compared with that in control group (P <0.01); the sperm aberration rates in 8 and 10 mmol/L trichloroethylene groups were significantly higher than that in control group (P<0.01). With the increase in exposure dose, the proportion of sperms with reduced mitochondrial membrane potential increased, and there were significant differences in sperm apoptosis rate between the 4, 6, 8, and 10 mmol/L trichloroethylene groups and control group (P<0.01).</p><p><b>CONCLUSION</b>In vitro exposure to trichloroethylene can reduce sperm motility and increase the aberration rate and apoptosis rate of sperms in male SD rats.</p>

A case of Keutel syndrome in child (review the literature) / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#The purpose is to report a calcification of the cartilaginous of the tracheobronchial case in child, and to recognize the clinical and imaging features on Keutel syndrome.@*METHOD@#A comprehensive analysis of the clinical data and X-ray,CT. Some literatures involving some symptoms of this child were reviewed.@*RESULT@#This patient diagnosed with Keutel syndrome finally.@*CONCLUSION@#When we meet calcification of the cartilaginous of the tracheobronchial patient in clinic, it may be Keutel syndrome.

Questionnaire-based survey of allergic diseases among children aged 0 - 14 years in the downtown of Beijing, Chongqing and Guangzhou / 中华儿科杂志

<p><b>OBJECTIVE</b>In recent years, the incidence of allergic diseases in China is increasing. With serious influence on the patients' quality of life and even life-threatening, now allergic diseases have become an important public health problem in our country. This survey aimed to obtain a general epidemic outline of children's major allergic diseases, i.e., childhood asthma, allergic rhinitis and eczema in the downtown of Beijing, Chongqing and Guangzhou.</p><p><b>METHOD</b>Childhood asthma questionnaire was established by Delphi method based on guidelines for childhood asthma diagnosis and prevention made by Chinese medical association in 2008 with reference to the International Study of Asthma and Allergies in Childhood questionnaire (ISAAC). This study was carried out from Oct. 2008-Mar. 2009, in Beijing, Chongqing and Guangzhou by a multi-stage sampling method, covering a sample of 24 290 children aged 0 - 14 years by a multi-stage sampling method, the population of children surveyed in the downtown of Beijing Chongqing and Guangzhou was 10 372, 9874 and 4072 respectively; of whom males were 5545, 5258, 2159, and females were 4917, 4588, 1877; the ratio of males to females in the three cities were 1.11:1, 1.15:1, 1.15:1; the average age of surveyed children were 7.63, 8.74 and 8.30 years old. All the questionnaire was filled in by the parents of children or other guardian of children.</p><p><b>RESULT</b>The self-reported prevalence of asthma in Beijing, Chongqing and Guangzhou was 3.15%, 7.45%, 2.09%, the self-reported prevalence of allergic rhinitis was 14.46%, 20.42%, 7.83%, and the prevalence of eczema was 20.64%, 10.02%, 7.22%. In the survey, asthma combined with allergic rhinitis had the highest rate, 49.54%, 50.14%, 34.83%, respectively in the 3 cities. The self-reported prevalence of asthma and allergic rhinitis was significantly higher in male than female. Besides, the self-reported prevalences of asthma and allergic rhinitis were relatively lower in children under 1 year and over 10 years of age.</p><p><b>CONCLUSION</b>The prevalence of asthma, allergic rhinitis and eczema and other allergic diseases in children 0 - 14 years of age in the three cities gradually increased, and the prevalence of allergic diseases in Chongqing was relatively higher.</p>

Current trends of the prevalence of childhood asthma in three Chinese cities: a multicenter epidemiological survey / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To analyze the prevalence of asthma and asthma related symptoms among children aged 0-14 years in three Chinese cities and to obtain a crude estimation of the trend of childhood asthma prevalence in China.</p><p><b>METHODS</b>A cross-sectional, population-based survey of prevalence of asthma was conducted in children aged from 0 to 14 years in 3 major cities of China (Beijing, Chongqing, and Guangzhou) with different geographic locations. All the subjects were randomly selected by a multi-stage sampling method. Three to five schools and kindergartens in 2 urban districts in each city were randomly selected for the survey, and a validated questionnaire that included the core questions of the International Study of Asthma and Allergies in Childhood, Phase III questionnaire and several additional questions were used. All questionnaires were completed by parents or guardians of the selected children. Children whose parents responded affirmatively to the question "Has your child ever been diagnosed as asthma by a doctor" were recognized as victims of asthma.</p><p><b>RESULTS</b>The prevalence of asthma in Beijing, Chongqing, and Guangzhou was 3.15%, 7.45%, and 2.09%, respectively. These values were significantly higher than those obtained 10 years ago in the national epidemiological survey in 2000 which used the same method of investigation and the same diagnotic criteria (χ²=3.938, P=0.047; χ²=73.506, P≤0.001; χ²=11.956, P=0.001, in each city). Of the asthmatic children 57.21%, 69.91%, and 60.00% had their first attack before the age of 3 in Beijing, Chongqing, and Guangzhou, respectively. Wheezing was the primary clinical manifestation for all asthmatic children, followed by persistent cough and repeated respiratory infections. Both the prevalence of asthma and asthma-related symptoms were statistically higher in males than in females.</p><p><b>CONCLUSION</b>The prevalence of childhood asthma is statistically higher than that 10 years ago in the three Chinese cities.</p>

Relationship of collagen type I alpha 1 and alpha 2 gene polymorphisms with bone mineral density / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate collagen type I alpha 1 (COL1A1) and alpha 2 (COL1A2) gene polymorphisms in Chinese and their relationship with bone mineral density.</p><p><b>METHODS</b>Totalling 628 residents of Han nationality in Guangzhou aged 53.4-15.9 (range 20-79) years were surveyed for COL1A1 and COL1A2 gene genotypes by polymerase chain reaction-restriction fragment length polymorphism. Bone mineral density of the lumbar vertebrae, greater trochanter, femur neck and Ward's triangle was measured by dual-energy X-ray absorptiometry.</p><p><b>RESULTS</b>COL1A1 Sp1 polymorphism was not found in these subjects, and the genotype of all samples were type SS. COL1A2 genotyping revealed the distribution of EE genotype in 49.7%, Ee in 40.9% and ee in 9.4% of the subjects. The frequency distribution of EcoR1 alleles followed the Hardy-Weinberg equilibrium. The mean bone mineral density did no significantly differ among these genotype groups (P>0.05 by analysis of variance).</p><p><b>CONCLUSION</b>COL1A1 Sp1 binding site polymorphism is absent and COL1A2 EcoR1 site polymorphism is not associated with bone mineral density in Chinese of Han nationality.</p>