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1.
Natural Product Sciences ; : 36-39, 2018.
Artigo em Inglês | WPRIM | ID: wpr-741599

RESUMO

Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1–9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a Discovery® C18 (4.6 × 250 mm, 5 µm) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were 10 µl and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin.


Assuntos
Boehmeria , Cromatografia Líquida , Coreia (Geográfico) , Rutina
2.
Chinese journal of integrative medicine ; (12): 591-599, 2018.
Artigo em Inglês | WPRIM | ID: wpr-691378

RESUMO

<p><b>OBJECTIVES</b>To investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models.</p><p><b>METHODS</b>MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92-62.50 μg/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 μg/mL. The expression of transforming growth factor β1 (TGF-β1), hepatocyte growth factor (HGF), β-catenin, substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed.</p><p><b>RESULTS</b>MSP (7.81 μg/mL) down-regulated TGF-β1 and up-regulated HGF and β-catenin in hDPCs (P<0.01). MSP (1000 μg/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01).</p><p><b>CONCLUSIONS</b>The MSP flower extract may have hair growth-promotion activities.</p>


Assuntos
Animais , Feminino , Humanos , Antioxidantes , Farmacologia , Contagem de Células , Proliferação de Células , MAP Quinases Reguladas por Sinal Extracelular , Metabolismo , Flores , Química , Folículo Piloso , Biologia Celular , Fator de Crescimento de Hepatócito , Metabolismo , Mastócitos , Biologia Celular , Camundongos Endogâmicos C57BL , Fosforilação , Extratos Vegetais , Farmacologia , Poaceae , Química , RNA Mensageiro , Genética , Metabolismo , Pele , Metabolismo , Fator de Células-Tronco , Metabolismo , Estresse Psicológico , Patologia , Substância P , Metabolismo , Fator de Crescimento Transformador beta , Genética , Metabolismo , Fator A de Crescimento do Endotélio Vascular , Genética , Metabolismo , beta Catenina , Metabolismo
3.
Natural Product Sciences ; : 134-139, 2016.
Artigo em Inglês | WPRIM | ID: wpr-221212

RESUMO

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β-sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, ¹H-, and ¹³C-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.


Assuntos
Boehmeria , Cromatografia , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Compostos Fitoquímicos , Plantas , Rutina , Urticaceae
4.
Natural Product Sciences ; : 82-86, 2015.
Artigo em Inglês | WPRIM | ID: wpr-182837

RESUMO

Phytochemical constituents were isolated from the fruits of Acanthopanax chiisanensis by repeated column chromatography. Their structures were identified as beta-sitosterol (1), daucosterol (2), sesamin (3), chiisanogenin (4), and 22alpha-hydroxy chiisanogenin (5) by spectroscopic analysis (MS, 1H-, and 13C-NMR). Compounds 1 - 5 were isolated for the first time from the fruits of A. chiisanensis.


Assuntos
Eleutherococcus , Araliaceae , Cromatografia , Frutas , Terpenos
5.
Natural Product Sciences ; : 66-70, 2015.
Artigo em Inglês | WPRIM | ID: wpr-102960

RESUMO

Content analysis of flavonoids (epicatechin, epicatechin gallate, and rutin) present in the leaves of Boehmeria nivea (originating from Geumsan-myeon, Biin-myeon, Hansan-myeon, and Baeksu-eup) and their commercial products (ramie tteok, ramie songpyeon, ramie bory-tteok, and ramie tea) was conducted by HPLC. The content of epicatechin, epicatechin gallate, and rutin was highest in the leaves of B. nivea from Geumsanmyeon (0.138 mg/g), Baeksu-eup (1.654 mg/g) and Geumsan-myeon (12.205 mg/g), respectively. With respect to commercial products, the content of epicatechin and epicatechin gallate was highest in ramie tea, with concentrations of 1.879 and 1.090 mg/g, respectively. Given these flavonoid concentrations, B. nivea leaf extracts have the potential to be used as additives in natural medicinal products, health supplements, and beverages.


Assuntos
Bebidas , Boehmeria , Catequina , Cromatografia Líquida de Alta Pressão , Flavonoides , Rutina , Chá
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