Qualitative and quantitative analysis of aristolochic acids in Chinese materia medica and traditional Chinese patent medicines / 中成药

AIM: To establish identification and determination methods for aristolochic acids. METHODS: The extracts of the sample were developed on silica gel G F254 plate, using supernatant layer of the mixture of toluene-ethyl acetate-water-formic acid (20 ∶10 ∶1 ∶1) as the mobile phase. Chromatogram system:gradient elution with mobile phase consisting of (A) 1% acetic acid in water and (B) methanol was used. The initial condition was at 40% of B and gradient up to 100% B in 15 minutes before returning to the initial conditions. Detection was at 310 nm. The column temperature was set at 40℃ and flow-rate was set at 1.0 mL/min. RESULTS: TLC:the Rf value of aristolochic acid Ⅰ was 0.50 and aristolochic acid Ⅱ was 0.53. The results of assay were shown as follow: The average recovery of aristolochic acid Ⅰ was 103.3% (RSD=0.98%). The average recovery of aristolochic acid Ⅱ was 95.97% (RSD=1.2%). CONCLUSION: It reveals that the methods we described are accurate, rapid and reproducible.