Investigation on Mechanisms of Osteoking Against Four Types of Bone Injury Diseases Based on Heterogeneous Information Network with Concept of "Treating Different Diseases with an Identical Treatment" / 中国实验方剂学杂志

ObjectiveTo elucidate the mechanism of Osteoking against fracture， femoral head necrosis， osteoarthritis， and lumbar disc herniation by integrating heterogeneous information network mining and experimental validation. MethodOn the basis of the disease-related database and transcriptome expression profiling dataset， as well as the ETCM database， the gene sets related to four target diseases and the candidate target spectrum of Osteoking were obtained through the integration and analysis of bioinformatics data， and a "disease-syndrome-formula-target-pathway-effect" heterogeneous information network was constructed. In addition， by functional enrichment analysis， the core targets of Osteoking in interfering with the imbalance network of four kinds of bone injury diseases， the biological pathways involved， and the corresponding clinical symptoms were screened， and they were verified in animal experiments. ResultHeterogeneous information network mining indicates that Osteoking may commonly reverse the imbalance networks of fracture， femoral head necrosis， osteoarthritis， and lumbar disc herniation via regulating cell function and activity， inhibiting inflammatory response， reducing bone destruction， and improving the immune function of the body by modulating relevant core candidate targets such as RAC-alpha serine/threonine-protein kinase （Akt1）， catenin beta-1 （CTNNB1）， epidermal growth factor receptor （EGFR）， heat shock protein 90-alpha （HSP90AA1）， and phosphatidylinositol 3-kinase catalytic subunit alpha isoform （PI3KCA）， as well as related biological pathways such as phosphatidylinositide 3-kinases/protein kinase B （PI3K/Akt）， janus kinase/signal transducer and activator of transcription （JAK/STAT）， tumor necrosis factor （TNF）， nuclear factor kappa-B （NF-κB）， and Toll-like receptors. In particular， Osteoking may improve the blood supply of the fracture end by regulating blood circulation at the target site of the disease， and it may maintain the balance of bone metabolism by regulating hormone-related pathways to promote fracture healing. In addition， Osteoking may relieve lipid metabolism disorders by targeting and regulating lipid-related pathways， accelerate bone formation and bone repair， and delay the progression of femoral head necrosis. Osteoking may relieve the symptoms of pain by acting on neurological pathways to reduce local nociceptive stimulation in patients with osteoarthritis and lumbar disc herniation. Further experimental validation demonstrates that the PI3K/Akt signaling pathway is the most significantly enriched pathway for the key network targets of Osteoking for the four diseases. The candidate target of Osteoking may have the strongest association with the network of fracture-related genes. Therefore， this study chooses fracture as the target disease to verify the efficacy of Osteoking. The results show that Osteoking can accelerate bone formation and promote fracture healing by inhibiting the activation of the PI3K/Akt signaling axis. ConclusionThe study shows that the main mechanism of "treating different diseases with an identical treatment" of four bone injury diseases with Osteoking involves cell function regulation and immune inflammation-related signaling pathways. Further experimental validation identifies that the PI3K/Akt signaling axis may be one of the key pathways of Osteoking to promote bone regeneration， bone reconstruction， and bone metabolism homeostasis.

Design of norms of Gong's nonverbal intelligence test for Chinese Han elderly in Luoyang / 中国组织工程研究

BACKGROUND: Gong's nonverbal intelligence test(GNIT) is a nonverbal and cross-cultural test of intelligence designed primarily to suit the use in the minority groups and those with linguistic difficulties by Professor Gong Yao-xian. At present 5 unified norms of the minority groups as well as norms of Chinese Han children and adult aged below 55 years have been developed, but the norms for the elderly above 56 years of age are not available. Nonverbal intelligence test is especially suitable for the elderly and has therefore wide applications.OBJECTIVE: To establish regional norms of the GNIT for healthy elderly people above 56 to enable the completion of nonverbal intelligence test norms suitable for each age groups.DESIGN: A controlled study with stratified sampling of healthy elderly retirees over 56 years of age in the community.SETTING: Department of Physiology in Medical College of Henan University of Science and Technology; Center for Psychology, Xiangya Second Hospital,Central South University.PARTICIPANTS: Healthy elderly retirees above 56 years of age in Luoyang cityor the counties were selected on a voluntary basis with stratified sampling according to the proportion of each region. The selected subjects aged between 56 and 101 years, and 8 age groups were divided at the interval of 5 years(about 200 people in each age group). Totally 1 460 subjects were selected, including 679 male and 781 female subjects.METHODS: With the instruments and manual of GNIT, the subjects were tested for color identification, classification, blank-filling with numbers or graphs, characters and encoding tests. The results were original scores, scale scores and intelligence quotient.MAIN OUTCOME MEASURES: The norms of GNIT for the elderly above 56 years of age.RESULTS: The norms included three scores, namely the original scores, scale scores and standard deviation scores. To test of reliability and validity of the norms, retest was performed in 40 1 or 2 weeks after the initial test, resulting in the test-retest reliability coefficient ranging from 0.59 to 0. 89, with the total test-retest reliability coefficient of 0.83. The correlation coefficients the original scores between the subtests ranged from 0. 35 to 0. 63, and the correlation coefficients between the standard deviation scores of the verbal intelligence quotient(VIQ), performance intelligence quotient(PIQ) and full intelligence quotient(FIQ) of Wechster intelligence scale for children(WAIC-RC) were 0.43,0.51 and 0.54 respectiyely, based on a study of a sample of 50 elderly subjects.CONCLUSION: Primary regional norms of GNIT for elderly subjects above 56 years of age have been established to measure mainly the perception and reasoning factors loaded with also visual motor psychological factors and abstract summary factors, with satisfactory reliability and validity equivalent to similar tests.

Treatment of 200 Cases of Asthma by Fat-cut Therapy / 针灸推拿医学（英文版）

After local anesthesia, the skin of Shanzhong (CV 17) was incised 2 cm in length to expose the subcutaneous fat fully, and after cutting away local fat, the handle of the forceps was used inside the incision directly for stimulation and then the incision was sutured. In 200 cases of asthma patients, 100 cases were cured, and 95 cases were improved and 5 cases failed, with the effective rate in 97.5%.

Effect of Low-temperature Environment on Contents of Serum Protein, Blood Sugar and Calcium in Rabbits / 环境与健康杂志

Objective To study the effects of low-temperature environment on the contents of serum protein, serum calcium and blood sugar in rabbits. Methods The rabbits were divided into three experiment groups, which were exposed to environment at 2, -4 and -6 ℃ respectively and one control group exposed to environment at room temperature of 16 ℃. After exposure for 30～60 min, the peripheral blood samples were collected from rabbits for determination of levels of blood sugar, serum protein and calcium. The anal temperatures and ECG of rabbits were also examined. Results The levels of blood sugar, serum protein and calcium of rabbits in experiment groups decreased with the decrease of environmental temperatures, and revealed significant differences compared with those of control group(P

EXPERIMENTAL. STUDY ON THE CHANGE OF K~+/Na~+ Mg~(2+)/Ca~(2+)、Zn~(2+)/Cu~(2+)RATIO IN CARDIAC MUSCLE WITH ACUTE MYOCARDIAL IN FARCTION / 中国法医学杂志

Using the animal model of acute myocardial infarction of rabbit after left coronary artery ligation, the authors studied the reltionship between the change of myocardial K~+/Na~+ ,Mg~(2+)/Ca~(2+), and Zn~(2+)/Cu~(2+) ratio and the time after acute myocardial infarction. The results demonstrated that. (1)the K+/Na~+ and Mg~(2+)/Ca~(2+)ratio dropped with prolongation of time myocardial ischemia. There was significant (P0.05)difference of Zn~(2+)/Cu~(2+) ratio between the experimental groups and the control group, but Zn~(2+)/Cu~(2+) ratio showed a tendency of dropping with prolongation of time of ischemia in the experimental group. At 120 min of myocardial ischemia,the Zn~(2+)/Cu~(2+) ratio was abour 1/2 as those at 15min, 30min, and 60min(P