Effect of percoll wash on sperm motility in artificial insemination

In this work the semen of 25 patients with initial motility 60% or less were subjected to Percoll gradient wash for sperm preparation for assisted reproductive technology. We have assessed the three motility parameters [percentage, types, longevity] before and after the wash. The mean sperm number was 50.9 +/- 16.4 million/ml. The initial sperm motility before the wash was ranging from 5% to 60% with a mean 41.12% +/- 16.44%. After percoll gradient wash sperm rnotility has been increased to 61.8 +/- 29.96% [P < 0.0025]. Percoll gradient wash eleminated sperms with motility grade O and grade 1. only sperms with motility grades 2,3 and 4 were present. We recommend the use of Percoll wash for sperm lipreparation especially in patients with asthenospermia whom will be treated by AIH

Unexplained infertility: identification by a modified immunoradiometric assay

Several methods have been employed to measure antisperm antibodies with variable sensitivity and specificity in serum and secretion of infertile patients. All of them are not precise means for identification of the presence of antisperm antibodies for patients with unexplained infertility [Gilbert and Haas, 1980]. Therefore, we applied the modified radiolabeled antiglobulin test that has been used successfully to identify and quantitate the antibodies directed towards other human cell surface [Clarke, 1988]. We studied 118 patients in different groups to quantitate the circulating antisperm antibodies using the modified procedure. The obtained data revealed that; the highest and the most significant incidence were found in the patient's secretions [Semen and cervical mucus] with unexplained infertility, and also in the group of males with varicocele. Therefore, It is greatly advisable to use the modified radiolabeled technique as a quantitative assay, and then to help manage infertility in patients with unexplained an mediated infertility