RESUMO
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
Assuntos
Animais , Centrifugação com Gradiente de Concentração , Cryptosporidium/crescimento & desenvolvimento , Meios de Cultura , Técnicas de Cultura , Fezes/parasitologia , Camundongos/parasitologia , Oocistos , Contagem de Ovos de Parasitas , Doenças dos Roedores/parasitologiaRESUMO
Toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. Live tachyzoites derived from serial passage in HeLa culture were used in the Sabin-Feldman dye test for detection of Toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (Prion nailurus viverrina), one leopard (Panthera pardus), two flat-headed cats (Prion nailurus planiceps), 6 tigers (Panthera tigris), two leopard cats (Felis bengalensis), two clouded leopards (Felis nebulosa), 3 pumas (Puma concolor), and 4 jungle cats (Felis chaus). Antibodies to Toxoplasma gondii were founded in 9 of 21 felids (42.8%). This study revealed that cell culture-derived tachyzoites can be used successfully as a source of live organisms in a gold standard Sabin-Feldman dye test, which is simpler, cheaper and less ethically sensitive than in vivo inoculation.
Assuntos
Animais , Anticorpos Antiprotozoários/sangue , Técnicas de Cultura , Felidae/parasitologia , Prevalência , Tailândia/epidemiologia , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/epidemiologiaRESUMO
The Mongolian gerbil (Meriones unguiculatus) is susceptible to infection with Giardia duodenalis trophozoites. Each animal was orally infected with 0.5 ml Diamond's TYIS-33 culture medium containing 10(6) trophozoites. Cysts were then collected and concentrated by sucrose gradient centrifugation. G. duodenalis cysts were first observed in feces on day 5 post-infection. The characteristic of G. duodenalis infection in gerbils was intermittent cyst release. The range in the number of cysts released per gerbil for a 4-hour collection period was 0-1.5 x 10(3).