Validation of UV Spectrophotometric Method of Analysis of some Marketed Perfloxacin in Nigeria

Background: Acquiring sophisticated LC instruments by most third world laboratories is capital intensive.Literatures on simple spectrophotometric analytical methods for pefloxacin are scarce. Objectives: The present study was undertaken to develop and validate a simple and economic UV spectrophotometric method for estimating pefloxacin mesylate (PFM) in dosage preparations.Methods: Using a JENWAY spectrophotometer at predetermined emax of 277nm with 1 v/v aqueous glacialacetic acid as blank; the method was validated for linearity; accuracy; precision; reproducibility; and specificity asper International Conference on Harmonization (ICH) guidelines and used to determine the content of pefloxacinin seven marketed brands in Nigeria. Results: The method exhibited good linearity over a range of 0.40-12.0 ?g/ml (regression equation: y = 0.0859x+0.0211 ; r=0.999). Mean recovery accuracy (99.183 ) and assay result in the range of 100.5- 110.17 for these lected brands were not significantly different at p=0.05. The coefficient of variation (CV) for both intra andinter-day were below 7 . The method was specific for pefloxacin in the presence of common excipients Conclusion: The method gave good validation results and could be employed for routine analysis of PFM incommercial formulations

Evaluation of phytochemical and antimicrobial properties of leaf extract of Tapinanthus sessilifolius (P. Beauv) van Tiegh.

Leaf extracts of T. sessilifolius growing on five different host plants (Psidium guajava, Citrus lemon, Vernonia amygdalina, Persea americana and Jatropa curcas) were evaluated for antimicrobial activity of the plant. Powdered leaves of T. sessilifolius collected from each host plant was divided into two portions. One portion was used for aqueous infusion and the other portion was successively extracted with hexane, ethylacetate and methanol. Infusion of aqueous extract of powdered leaves did not show antimicrobial effect even at the concentration of 1000 and 2000 microg/ml on test microorganisms (Staph. aureus, E. coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans). However in broth culture, methanolic and hexane extract had MIC range of 62.5-500 microg/ml and ethylacetate extract had 250-500 microg/ml. Phytochemical screening of leaf samples of T. sessilifolius collected from different host plants showed positive test for hydrolysable tannins, saponins, flavonoids, terpenes, cardiac glycoside, reducing sugars and proteins. LD50 concentration was found to be > 1.500 mg/kg for samples from P. guajava; 489.89 mg/kg for J. curcas and C. lemon; and 692 mg/kg for V. amydalina in mice.