Studies on chemical constituents in bark of Larix olgensis var. koreana / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in bark of Larix olgensis var. koreana.</p><p><b>METHOD</b>The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).</p><p><b>RESULT</b>Eight compounds were isolated and identified as isopimaric acid (I), beta-sitosterol (II), 24R,5alpha-stigmast-3,6-dione (III), larixol (IV), ferulic acid (V), lariciresinol (VI), secroisolariciresinol (VII) and isolariciresinol (VIII).</p><p><b>CONCLUSION</b>All the compounds were isolated from this plant for the first time.</p>

Studies on the chemical constituents in roots of Daphne odora var. atrocaulis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from Daphne odora var. atrocaulis.</p><p><b>METHOD</b>The chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physicochemical properties.</p><p><b>RESULT</b>Sixteen compounds were obtained and nine of them were identified as beta-sitosterol, 4-hydroxy ethylbenzoate, (2E),-2-propenoic acid,3-(3,4-dihydroxyphenyl)-decosylester, genkwanin, 2,4-dihydroxypyrimidine, daphnetin, daphnoretin, 5,7,4'-trihydroxyflavone-3ol, daucosterol.</p><p><b>CONCLUSION</b>Seven compounds were obtained from D. odora var. atrocaulis. for the first time.</p>

DNA microarray analysis of protective mechanism of buyang huanwu decoction on focal cerebral ischemia/reperfusion in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To analyse the effects of buyang huanwu decoction (BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray.</p><p><b>METHOD</b>cDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given p.o. after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray.</p><p><b>RESULT</b>Up-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group.</p><p><b>CONCLUSION</b>BYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats, due to its mechanism of protecting cerebral ischemia/reperfusion injury.</p>

Studies on chemical constituents in bulbs of Bolbostemma paniculatum / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of the bulbs of Bolbostemma panicultum.</p><p><b>METHOD</b>The compounds were isolated by column chromatography on silica gel, C18, Sephadex LH-20 separately and their structures were elucidated by chemical and spectroscopic technologies.</p><p><b>RESULT</b>Eight compounds were isolated and identified as maltol(I), emodin(II), cucurbitacin B(III), cucurbitacin E(IV), stigmasta-7, 22, 25-triene-3-ol(V), stigmasta-7, 22, 25-triene-3-nonadecanoic acid ester(VI), stigmasta-7, 22, 25-triene-3-O-beta-D-glucopyranoside(VII), stigmasta-7, 22, 25-triene-3-O-beta-D-(6'-palmitoyl) glucopyranoside(VIII).</p><p><b>CONCLUSION</b>I-VIII were obtained from this plant for the first time; VI and VIII are new compounds.</p>

Studies on the lignans from Patrinia scabra / 药学学报

<p><b>AIM</b>To study the lignans from Patrinia scabra Bunge.</p><p><b>METHODS</b>The constituents were separated and purified by column chromatography with silical gel, RP-silical gel and Sephadex LH-20. Their structures were identified on the basis of spectral data (IR, MS, 1HNMR, 13CNMR, HMQC and HMBC).</p><p><b>RESULTS AND CONCLUSION</b>A new lignan was obtained and its structure was elucidated as 4-[1-ethoxyl-1-(4-hydroxy-3-methoxy)benzyl]methyl- 2-(4-hydroxy-3-methoxy)benzyl-3-hydroxymethyl-tetrahydro-furan (2), along with three known lignans, lariciresinol (1), isolariciresinol (3) and nortracheloside (4).</p>