Metabolic Syndrome and Risk of Gastric Cancer: A Meta-analysis / 肿瘤防治研究

Objective To evaluate the relation between metabolic syndrome (MetS) and gastric cancer (GC) based on a meta-analysis. Methods The case-control studies about the relation between MetS and GC were retrieved from CNKI, WanFang Data, VIP, CBM, Web of Science, The Cochrane Library and PubMed database. The retrieval time was from inception to June, 2020. Two researchers independently screened the literatures, extracted the data and evaluated the quality of the included studies. The meta-analysis of the included literatures was conducted by the Stata 12.0 software. Results A total of six literatures, involving 43617 participants, were included. The results of meta-analysis showed that there was no statistically significant difference between GC and non-GC groups in the risk of hyperglycemia (OR=1.24, 95%CI: 0.59-2.61), hypertension (OR=1.40, 95%CI: 0.60-3.25) or MetS (OR=0.79, 95%CI: 0.44-1.39), respectively. Conclusion MetS may not be related to the risk of GC.

Correlation between blood lipid levels and disease severity in imported malaria patients from Africa / 中华传染病杂志

Objective:To explore the clinical characteristics of imported malaria from Africa, and the correlation between blood lipids and disease severity.Methods:The clinical data of 172 imported malaria patients from Africa were collected from January 1, 2018 to January 31, 2021 in Guangzhou Eighth People′s Hospital, Guangzhou Medical University. The general conditions, clinical symptoms, laboratory tests, treatment and prognosis of the patients were analyzed retrospectively. The independent samples t-test, Mann-Whitney U test and binary multivariate logistic regression analysis were used for statistical analysis when appropriate. Results:A total of 172 patients were divided into 39 severe cases and 133 non-severe cases in this study. The main infection species was Plasmodium falciparum, with a total of 153 cases (89.0%), and the severe cases were all falciparum malarias. Compared with the non-severe group, the white blood cell count and triacylglycerol level were increased in the severe group.The differences were both statistically significant ( Z=2.397 and 4.368, respectively; both P<0.05). The hemoglobin, hematocrit, platelet count, high-density lipoprotein cholesterol, apolipoprotein A (ApoA)Ⅰ and ApoAⅠ/apolipoprotein B (ApoB) were significantly decreased in the severe group. The differences were all statistically significant ( Z=-4.473, -4.464, -4.750, -4.826, -5.488 and -4.419, respectively; all P<0.01). The total cholesterol and low-density lipoprotein cholesterol were also reduced in the severe group. The differences were both statistically significant ( t=3.817 and 5.285, respectively; both P<0.01). The area under receiver operator characteristic curve of high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triacylglycerol, ApoAⅠ, and ApoAⅠ/ApoB were 0.754, 0.727, 0.730, 0.789 and 0.733, respectively (all P<0.01). When the Youden index was at its maximum, the best cut-off value of ApoAⅠ was 0.535 g/L with sensitivity of the prediction for severe malaria of 79.5% and the specificity of 68.4%. ApoAⅠ had independent predictive value for severe malaria (odds ratio ( OR)=0.013, 95% confidence interval ( CI) 0.002 to 0.086, P<0.01), and stratified according to the best cut-off value, the risk of severe malaria when ApoA Ⅰ<0.535 g/L was 8.396 times of ApoA Ⅰ≥0.535 g/L ( OR=8.396, 95% CI 3.557 to 19.820, P<0.01). The case fatality rate of severe malaria was 2.6%(1/39). Conclusions:The imported malaria patients from Africa are mainly infected with Plasmodium falciparum and the case fatality rate of severe malaria is high. The high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triacylglycerol, ApoAⅠ, ApoAⅠ/ApoB of blood lipids show higher predictive value for severe malaria, among which, ApoAⅠ has independent predictive value for severe malaria. Changes in blood lipids will help to identify severe malaria as early as possible, improve the cure rate, and reduce the risk of death.

Improved synthesis process of optical-magnetic bimodal probe of Gd-4, 7-Bis-carboxymethyl-10-(2-fluoresceinthioureaethyl)-1, 4, 7, 10-tetraaza-cyclododec-1-yl-acetic acid complexes / 北京大学学报(医学版)

OBJECTIVE@#To improve the methods to synthesize and purify of optical-magnetic bimodal molecular probe of Gd-[4, 7-Bis-carboxymethyl-10-(2-fluorescein thioureaethyl)-1, 4, 7, 10-tetraaza-cyclododec-1-yl]-acetic acid complexes.@*METHODS@#Target compound (7), optical-magnetic bimodal molecular molecular probe, was synthesized by the use of 1, 4, 7, 10-tetraazacyclododecane (1) as starting material via substitution reaction, hydrolysis reaction, coupling reaction and complexation reaction with metal.@*RESULTS@#The synthetic route of Gd-[4, 7-Bis-carboxymethyl-10-(2-fluoresceinthioureaethyl)-1, 4, 7, 10-tetraaza-cyclododec-1-yl]-acetic acid complexes was improved. The optical-magnetic bimodal molecular probes were synthesized by substitution reaction, hydrolysis reaction, coupling reaction and complex reaction with metal respectively. For the improved route, the total yield could reach 34.6% which was higher than the original route (18.0%). The structures of those compounds were identified by 1H nuclear magnetic resonance, 13C nuclear magnetic resonance, and mass spectrometry. The improved route could avoid the uncontrollable disadvantage of the substitution reaction, this process could reduce the formation of impurities and made the purification process easier, and in the aspect of purification and separation, the preparative high-performance liquid chromatography with less sample loading and high cost was improved to a column chromatography with many sample loads and being easy to operate. Therefore, the use of column chromatography could be more conducive to mass production of the optical-magnetic bimodal molecular molecular probe.@*CONCLUSION@#The improved synthetic route improves the controllability of the reaction conditions and makes it easier to purify and separate the compounds. At the same time, the improved synthetic route can increase the total yield significantly. The optical-magnetic bimodal molecular probe can combine the living magnetic resonance imaging with the in vitro optical imaging to realize the dual synchronous detection of magneto-optics, so that the detection results of the living magnetic resonance imaging and the in vitro optical imaging are mutually verified. In other words, this synthetic optical-magnetic bimodal molecular probe will make the experimental results more accurate and reliable. In subsequent biological experimental studies, the optical-magnetic bimodal molecular probe can be applied to related research of brain structure and function, and the probe can be used for the brain-related diseases researches, such as brain tumors. after intravenous administration, and thus the optical-magnetic bimodal molecular probe can play an important role in medical treatment of brain tumors and cerebrovascular diseases.

Research on distribution of invention patents of Chinese materia medica for treating high mortality diseases in China--analysis mechanism based on IPC number A61P / 中草药

Based on the International Patent Classification (IPC) A61P, this paper analyzes the distributional therapeutic situation of Chinese materia medica (CMM) in China dynamically and statically from the perspective of invention patent, then the list of 10 groups of A61P with the largest amount of invention patents application and effective amount is compared with the list of high mortality diseases in China, so as to verify the idea whether the R&D focus of CMM is on high mortality diseases, and put forward suggestions for the R&D directions and strategies of CMM in the treatment of high mortality diseases. The research finds that the R&D of CMM mainly focuses on treating high mortality diseases, except for the malignant tumors and mental disorders. In addition, the intellectual achievement of CMM in the treatment of digestive system diseases is the dominant direction. Under the background of internationalization and standardization of CMM, China needs to strengthen the R&D investment direction of CMM in the treatment of malignant tumors, mental disorders and other fields. The quantity and quality advantages of CMM in the treatment of digestive system diseases will be of significance, thus it needs to actively carry out patent distribution abroad. The role of technical evaluation in the cultivation of high-quality and high-value patents should be attached importance.

Application value of high-frequency ultrasound in diagnosis of finger extensor tendon olisthy / 中国医学影像技术

Objective: To explore the application value of high-frequency ultrasound in diagnosis of finger extensor tendon olisthy. Methods: A total of 32 dorsal trauma in metacarpophalangeal joint patients with clinically suspected finger extensor tendon olisthy underwent high-frequency ultrasound. Olisthy in finger extensor tendon and causes of injuries were observed, and the results were compared with those of surgical operations. Results: Among all 32 cases, surgery treatment was performed in 25 patients, including 7 patients of partial olisthy in extensor tendon and 18 patients of complete olisthy, while 7 patients were found without olisthy (only tendon hood injury) and were followed up for observation. Utrasound missed 1 case with complete olisthy in finger extensor tendon and misdiagnosed 2 cases of complete olisthy as partial olisthy. The consistency of ultrasound examination and surgery results was well (Kappa=0.85, P<0.01). Conclusion: Ultrasound can show tendon hood tear and finger extensor tendon olisthy and judge the injury degree through dynamic observation, therefore providing important evidences for clinical treatment.

Observations on the Efficacy of Penetrating Moxibustion plus Short Joint Needling in Treating Restless Leg Syndrome / 上海针灸杂志

Objective To investigate the clinical efficacy of penetrating moxibustion plus short joint needling in treating restless leg syndrome (RLS).Methods Ninety RLS patients were randomized to treatment and control groups,45 cases each.The treatment group received penetrating moxibustion plus short joint needling and the control group,conventional medication (vitamin B tablets,vitamin B12 tablets and alprazolam tablets).The Restless Leg Syndrome Rating Scale (RLSRS) score and the Pittsburgh Sleep Quality Index (PSQI) score were recorded in the two groups before and after 30 days of treatment.The clinical therapeutic effects were compared between the groups.Results There were statistically significant pre-/post-treatment differences in the RLSRS and PSQI scores in both groups (P＜0.05).There were statistically significant post-treatment differences in the RLSRS and PSQI scores between the treatment and control groups (P＜0.05).The total efficacy rate was 95.6％ in the treatment group and 42.2％ in the control group;there was a statistically significant difference between the two groups (P＜0.05).Conclusion Penetrating moxibustion plus short joint needling is an effective way to treat RLS.

Analysis on health literacy and its influencing factors of enterprise employees in Minhang District of Shanghai / 复旦学报（医学版）

Objective To explore health literacy and the related factors among enterprise employees in Minhang District of Shanghai,and to provide scientific basis for the development of health literacy promotion strategies for occupational population.Methods Through cluster random sampling,15 manufacturing enterprises of Minhang District were selected.The information of health-supportive environment in the workplace was collected through field observation.And the general demographic characteristics,occupational characteristics,health literacy and self-efficacy of the subjects were collected through the survey.Results Only about 28.36％ of the 1 421 employees surveyed possessed basic health literacy.Education level,marital status,working nature and payment pressure were the factors affecting the health literacy level of respondents.Compared with the employees who had at least a bachelor degree,the health literacy level of the employees who had a lower degree of bachelor was relatively low (P＜0.05).The health literacy level of employees in non-marital status was relatively lower than those who were married (OR =1.515,P =0.021).Compared with the managers,the health literacy levels of clericals,production operators and logistical service personnel were low (P ＜0.05).Employees who had the pressure to pay possessed a low level of health literacy (OR =1.641,P＜0.001).Conclusions The health literacy level of employees in this region was better than the average level of Chinese citizen,but it was still not optimistic.The health literacy level of enterprise employees was affected by many factors,and these factors were closely related.Improving the health supportive environment in the workplace may be an effective strategy for promoting health literacy among occupational populations.

Analysis on health literacy and its influencing factors of enterprise employees in Minhang District of Shanghai / 复旦学报（医学版）

Objective To explore health literacy and the related factors among enterprise employees in Minhang District of Shanghai,and to provide scientific basis for the development of health literacy promotion strategies for occupational population.Methods Through cluster random sampling,15 manufacturing enterprises of Minhang District were selected.The information of health-supportive environment in the workplace was collected through field observation.And the general demographic characteristics,occupational characteristics,health literacy and self-efficacy of the subjects were collected through the survey.Results Only about 28.36％ of the 1 421 employees surveyed possessed basic health literacy.Education level,marital status,working nature and payment pressure were the factors affecting the health literacy level of respondents.Compared with the employees who had at least a bachelor degree,the health literacy level of the employees who had a lower degree of bachelor was relatively low (P＜0.05).The health literacy level of employees in non-marital status was relatively lower than those who were married (OR =1.515,P =0.021).Compared with the managers,the health literacy levels of clericals,production operators and logistical service personnel were low (P ＜0.05).Employees who had the pressure to pay possessed a low level of health literacy (OR =1.641,P＜0.001).Conclusions The health literacy level of employees in this region was better than the average level of Chinese citizen,but it was still not optimistic.The health literacy level of enterprise employees was affected by many factors,and these factors were closely related.Improving the health supportive environment in the workplace may be an effective strategy for promoting health literacy among occupational populations.

Inhibitors of JAK2/STAT Signaling Pathway and Hematologic Malignancies-Review / 中国实验血液学杂志

Jauns kinase (JAK)/transducer and activator of transcription（STAT） pathway is a classical approach to study the rapid changes of the gene expression in specific target cells by a variety of extracellular signals. The JAK and STAT transfer cytokine receptor signaling plays a unique role in multiple cellular and molecular biological changes.The abnormal signal of JAK/STAT pathway will lead to the hematopoietic abnormalities.Studies had shown that the abnormal activation of JAK2/STAT signaling pathway are in many kinds of malignant hematological diseases, such as in acute lymphoblastic/myeloid leukemia, chronic myeloid leukemia, lymphoma, myelodysplastic syndromes, myeloprofilerative neoplasm, especially in the patients of myeloproliferative neoplasm(MPN) with JAK gene mutation(JAK2V617F), this mutation has an important value for MPN diagnosis. At present, the effect of the specific inhibitors of JAK2 has showed good perspective, which had been applied to clinic treatment and achieved remarkable curative effect. In this review, the JAK2/STAT signaling transduction, the JAK2 signal and hematologic malignancies, the kagulation of signaling pathway and the inhibitors of JAK2/STAT signaling pathway are summarized.

Study on the course reformation of medical laboratory science after changing five-year system to four-year system / 中华医学教育探索杂志

To adapt to the system reformation of medical laboratory science from five academic years to four academic years and to meet the new professional technology-oriented requirements, the medical laboratory science institute of Guangdong Medical College has carried out a comprehensive reform of curriculum system. This paper has analyzed the current problems in the school medical ex-amination and explored the curriculum system reform from three respects such as adjusting curriculum by restructuring and integrating programs, implementing modular teaching to build its characteristics and strengthening practice teaching.And it has also explored the full assessment mode by optimizing the traditional one-stop assessment.

The effect of Shanzha Xiaozhi capsule on intima-media thickness and plaques score of carotid artery in patients with non-acute phase coronary heart disease / 中国中西医结合急救杂志

Objective To investigate the effect of Shanzha Xiaozhi capslue on intima-media thickness(IMT) and plaques score of carotid artery in patients with non-acute phase coronary heart disease and traditional Chinese medicine(TCM)syndrome of phlegm and blood stasis. Methods A prospective study was conducted to carry out a research on 102 patients with non-acute phase coronary heart disease and TCM syndrome of phlegm and blood stasis. They were randomly divided into two groups:a control group(50 cases)treated with conventional western medicine alone and a observation group(52 cases)which was treated by both conventional western medicine and Shanzha Xiaozhi capslue(the main TCM ingredients:Shanzha,Dahuang)0.7 g,3 times a day,the therapeutic course being 6 months in both groups. The cardiocerebral vascular incidences of the two groups were observed,in the mean time,the carotid artery IMT and plagues score were registered,the scores of phlegm stagnation syndrome and blood-stasis syndrome were measured,adverse reaction was observed and compared in both groups before and after treatment. Results After treatment,the occurrence of major adverse acute cardiovascular and cerebrovascular events in observation group were reduced significantly compared with those in the control group(5.77%vs. 20.00%,P<0.05). The IMT and plaques score of carotid artery and the scores of phlegm stagnation syndrome and blood-stasis syndrome were all decreased obviously compared to those before treatment,and the effect was more remarkable in the treatment group〔IMT of carotid artery(mm):0.80±0.13 vs. 0.95±0.12,the plaques score:1.35±0.65 vs. 1.75±0.88, phlegm syndrome score:20.98±6.42 vs. 35.55±9.22,blood-stasis syndrome score:23.23±5.12 vs. 28.95±6.38, P<0.05 or P<0.01〕. Abdominal pain appeared in 2 patients of observation group without other adverse reactions. Conclusion Shanzha Xiaozhi capsule can stabilize atherosclerotic plaque and reduce the occurrence of acute adverse cardiocerebral vascular events in patients with non-acute phase coronary heart disease and phlegm and blood stasis syndrome possibly by removing phlegm and blood stasis to decrease the IMT and plaques score of carotid artery.

Effect of Shanzha Xiaozhi Capslue on Stability of Atherosclerosis Plaques in Patients with Metabolic Syndrome and Carotid Atherosclerosis / 中国中医药信息杂志

Objective To investigate the effect of Shanzha Xiaozhi Capslue on stability of atherosclerosis plaques in patient with metabolic syndrome (MS) and carotid atherosclerosis (CAS). Methods Totally 106 patients with MS and CAS were randomly divided into 2 groups with 55 cases in the treatment group and 51 cases in the control group. The control group improved the way of life and control of relevant risk factors, while the treatment group was treated with Shanzha Xiaozhi Capslue additionally for 6 months. The occurrence of major adverse cardiovascular and cerebrovascular events of the two groups was compared. The intima-media thickness, plaques grade of carotid artery and the changes of Hs-CRP, Hcy, insulin resistance were measured before and after the treatment. Results After treatment, the occurrence of major adverse cardiovascular and cerebrovascular events in the treatment group reduced significantly compared with the control group (P=0.047). The intima-media thickness and plaques grade of carotid artery in the treatment group decreased significantly compared with the control group (P<0.05), and the serum levels of Hs-CRP, Hcy, insulin resistance were decreased significantly compared with the control group (P<0.01). Conclusion Shanzha Xiaozhi Capslue can decrease the intima-media thickness and plaques score of carotid artery, stabilize atherosclerotic plaque, thus reduce the occurrence of major adverse cardiovascular and cerebrovascular events of patients with MS and CAS.

Dietary calcium decreases plasma cholesterol level only in female but not in male hamster fed a high cholesterol diet / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the effect of dietary calcium on plasma lipoprotein profile in castrated and ovariectomized hamsters.</p><p><b>METHODS</b>Male, castrated, female and ovariectomized hamsters (n=36 each group) were randomly divided into three sub-groups (n=12) and fed one of the three diets containing 0, 2, and 8 g calcium per kg diet for a period of six weeks. Changes in plasma lipoprotein profile were monitored at the end of week 0, 3 and 6.</p><p><b>RESULTS</b>Plasma total cholesterol (TC), non-high density lipoprotein cholesterol (non-HDL-C), triacylglycerols (TG) and TC/HDL-C were decreased only in intact female and ovariectomized hamsters. In contrast, three levels of dietary calcium had no effect on lipoprotein profiles in both intact male and castrated hamsters.</p><p><b>CONCLUSION</b>Beneficial modification of lipoprotein profile by dietary calcium was gender-dependent at least in hamsters.</p>

Nicotine inhibits histone deacetylase 6 activity and chaperone-dependent activation of the glucocorticoid receptor in A549 cells / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Nicotine, a major component of tobacco, is the main cause of smoking addiction. It was found that asthmatic patients who smoke were insensitive to glucocorticoid treatment. In this paper, we investigated whether nicotine could inhibit histone deacetylase 6 activity (HDAC6) and chaperone-dependent activation of the glucocorticoid receptor (GR) in A549 cells. Furthermore, the expression level of heat shock protein 90 (HSP90) was determined.</p><p><b>METHODS</b>Quantitative real-time polymerase chain reaction was used to detect the levels of RNA transcription, and Western blotting was applied to analyze the levels of protein expression of HDAC6, GR, and HSP90 in A549 cells. Moreover, the effects of dexamethasone and trichostatin A were observed in A549 cells.</p><p><b>RESULTS</b>A549 cell proliferation was inhibited in the presence of nicotine, and the level of RNA and protein expression of HDAC6 and GR were down-regulated.</p><p><b>CONCLUSIONS</b>Nicotine could inhibit HDAC6 activity and chaperone-dependent activation of GR. This might be the main reason why asthmatic patients who smoke show insensitivity to the glucocorticoid treatment.</p>

Relationship between skin carotenoid levels and individual's lifestyle / 中华健康管理学杂志

Objective To explore the relation of skin carotenoid levels with lifestyle.Methods Complete clinical data of 493 adults who received health check-up in our hospital between December 2010 and December 2011were collected for this retrospective study.The relationship between skin carotenoid level and lifestyle was investigated.ResultsNo strong relationship between skin carotenoid level and age,education level,occupation and marriage status was found ( x2 values were 2.150,6.443,4.560 and 2.028,respectively; all P ＞0.05 ).However,skin carotenoid was significantly correlated with dietary variety,intake of fruits and vegetables,exercise frequency,cigarette smoking,passive smoking,mood,sleeping and gender( x2 values were 520.234,89.936,4.002,81.428,154.271,84.871,11.546 and 2.150,respectively ; all P ＜0.05 ).Conclusions Our data suggest a relationship between lifestyle and skin carotenoid level.Skin carotenoid assessment may help to detect bad lifestyle in time.

Aortic artery elastic lamina degradation, collagen remodeling, oxides stress and inflammation in the apolipoprotein E deficient mice with or without aortic banding / 中华心血管病杂志

<p><b>OBJECTIVE</b>To explore the elastic lamina degradation and the collagen remodeling of aortic artery as well as oxides stress and inflammation of the apolipoprotein (Apo E) deficient mice with or without experimental hypertension.</p><p><b>METHODS</b>Eighty male Apo E deficient mice were fed with high-fat diet beginning at six weeks of age. At 8-week old, they were randomly divided into hypertension group and control group (n=40 each), the mice in hypertension group were subjected the suprarenal aortic constriction operation and then randomly divided into two subgroups: 15 weeks age and 30 weeks age groups. At the end of experiment, the vascular elastic lamina degradation and the content of collagen were determined by morphological method, plasma ICAM-1 level was measured by ELISA, and the rennin activity measured by radioimmunoassay, the superoxide anion detected by fluorescence, the MOMA-2 observed by immunofluorescence in all animals. mRNA expression of NF-κB P65 and MMP9 was detected by real-time PCR.</p><p><b>RESULT</b>In 15-week old group, the elastic lamina degradation Grade II and the intima-media thickness in the hypertension group were significantly higher than in the control group [(5.4±3.3)% vs. (8.9±2.5)%, P<0.05; (98.66±18.90) µm vs. (70.08±11.71) µm, P<0.05]. In 30-week old group, the elastic lamina degradation Grade III, the III type of collagen and the intima-media thickness were also significantly higher than in the control group [(15.2±3.7)% vs. (8.1±3.3)%, P<0.01; (23.00±7.73)% vs. (11.00±3.82)%, P<0.05; (147.31±22.60) µm vs. (103.98±17.21) µm, P<0.01]. The level of ICAM-1 in hypertension group was significantly higher than that of control group in both 15-week old and in 30-week old mice [(46.3±3.7) µg/ml vs. (40.6±5.7) µg/ml, P<0.05; (56.0±3.1) µg/ml vs. (45.2±2.8) µg/ml, P<0.05]. The superoxide anion, the MOMA-2, mRNA expression of NF-κB P65 and MMP9 in the hypertension group were significantly higher than in the control group in both 15-week old and in the 30-week old mice. The increase in hypertension group was more pronounced in the 30-week old mice than in the 15-week old mice.</p><p><b>CONCLUSION</b>The elastic lamina degradation and the collagen remodeling of aortic artery as well as oxides stress and inflammation are more significant in the Apo E deficient mice with hypertension than in control Apo E deficient mice.</p>

Quantitative evaluation of kidney cortex hemoperfusion with contrast-enhanced ultrasound after extracorporeal shock wave lithotripsy / 中国介入影像与治疗学

Objeetive To investigate the changes of kidney cortex hemoperfusion before and after extracorporeal shock wave lithotripsy (ESWL) with contrast-enhanced ultrasonography (CEUS) and time-intensity curve.Methods Thirty patients of renal calculi were treated with ESWL and examined with CEUS before and after ESWL.Renal cortex blood perfusion parameters of the lithotriptic areas,including the contrast agent arrival time (AT) ,time to peak (TTP) .peak intensity (PI) and velocity parameters (β) were quantitatively measured with ACQ software.Results The value of AT,TTP and β were not significantly different before and after ESWL (P＞0.05) .PI value after ESWL was lower than that before ESWL (P＜0.05) . Conclusion CEUS can quantitatively evaluate the changes of kidney cortex hemoperfusion after ESWL,and reflect the minor renal damage resulted from ESWL.CEUS can be used as a new method of observing and evaluating the renal damage caused by ESWL.

Color Doppler ultrasonic diagnosis of acute osteofascial compartment syndrome of forearm / 中国介入影像与治疗学

Objective To assess the application value of color Doppler ultrasound (CDU) in the diagnosis of osteofascial copmarttnent syndrome (OCS) of forearm.Methods A total of 19 patients highly suspected of or diagnosed as acute OCS of forearm underwent CDU in comparison with clinical diagnosis and therapy.The echoes of muscles,the diameter and change of flow Doppler spectra of radial artery and ulnar artery were observed.Results In 16 patients of OCS,most of the forearm muscle swelled (enlarged more than 1/2-2/3 area) with the muscle echo enhanced unevenly:the textures were unclear or disappeared;and fluid or hematom appeared in muscles;and the diameter of radial artery and ulnar artery diminished.The flow Doppler spectra of radial artery and ulnar artery appeared as bid-diastolic whole inverse wave,bip-,unid-,uniph-wave,unip-,unid-uniph-wave and venous like Doppler wave.Resistance index increased.Conclusion The acute OCS of forearm has some sonographic characteristics.CDU can be used to diagnose the acute OCS of forearm.

Determination of the trace levels of urinary fibrinopeptides by high-performance capillary electrophoresis / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish a high-performance capillary electrophoresis (HPCE)-based method for detection of trace amount of urinary fibrinopeptide A and B (FPA and FPB, respectively) as the specific molecular markers of thrombus formation in vivo.</p><p><b>METHODS</b>The HPCE system consisted of a 25 cm x 50 microm (inner diameter) coated capillary column, 0.1 mol/L phosphoric acid buffer (pH 2.5) and a UV-detector (wavelength at 190 nm). To improve the sensitivity and reproducibility, solid-phase extraction of FPA and FPB in the urine was performed using a Sep-pak C18 column, with a synthetical fibrinopeptide B-Tyr (FPB-Tyr) as the internal standard.</p><p><b>RESULTS</b>With this HPCE method, optimal separations of FPA, FPB and FPB-Tyr was achieved within 16 min, with the migration time of 7.28 min, 14.31 min and 15.22 min, respectively. The adjusted peak area ratios of FPA or FPB and the internal standard showed good linearity with the corresponding concentrations of FPA or FPB spiked in the urine(R>0.99). Under the above chromatography conditions, the minimum detection concentration of FPA and FPB in untreated urine was 30 microg/L and 40 microg/L, respectively, and the assay precision and recovery of FPA and FPB were acceptable.</p><p><b>CONCLUSION</b>The method we established is reliable and specific for separation and identification of fibrinopeptides and other bioactive peptides.</p>

Study on the anti-inflammatory effect of proanthocyanidins and its mechanism / 国际检验医学杂志

Objective To investigate the anti-inflammatory effect of proanthocyanidins and its mechanisms.Methods Inflammation models such as dimethylbenzene-indueed ear swelling and carrageenan-induced hind paw edema in mice and rats were prepared.The contents of PGE2 in exudate from edema paws of rats were measured by ultraviolet spectrophotography and the protein expression of COX-2 in edema paws of rats by Western-blot and immunohistoehemistry(IHC)assay.Results Pro-anthocyanidins remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of10 mg/kg and 20 mg/kg;paw edema of rats induced with carrageenan was significantly inhibited byproanthocyanidins 5,20 mg/kg ip from 2 to 5 h;proanthoeyanidins 5,20 mg/kg ip reduced the contents of PGE2 in exudate from edema paws of rats induced by carrageenan;proanthocyanidins 5,20 mg/kg ip inhibited the protein expression of COX-2.Conclusion Proanthocyanidins has an anti-inflammatory effect in vivo which may be related to inhibition of protein expression of COX-2 and downregutation of PGE2 biosynthesis.