Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle / 中国骨伤

<p><b>OBJECTIVE</b>To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.</p><p><b>METHODS</b>The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.</p><p><b>RESULTS</b>Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).</p><p><b>CONCLUSION</b>Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.</p>

Biomechanical study and clinical choice of different fixation devices to treat femoral fracture / 医用生物力学

Objective To investigate the biomechanical properties of transverse fractured femurs when fixed by TiNi shapememory alloy embracing plate (TiNi SMA), limitedcontact dynamic compression plate (LCDCP) and static interlocking intramedullary nail (SIiN), respectively, so as to provide the theoretical evidence for orthopedic surgeons in clinical application. Methods Eight pairs of cadaveric femurs were used with one side randomly fixed by TiNi SMA and SIiN and the other side fixed by LC-DCP. Then the axial compression test, threepoint bending test, and torsion test were conducted. Results For axial compression test, the strains of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). The displacements of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). For three-point bending test, the displacements of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). For torsion test, the torsion angels of TiNi SMA group were similar to those of SIiN group (P>0.05), but the torsion angles of LC-DCP group were smaller than those of TiNi SMA group（≥3 N•m）and SIiN group (P<0.05). Conclusions When the fractured femurs are fixed by three internal fixation devices, the stability of TiNi SMA group is weakest, SIiN group being better, and LCDCP group being best. The axial micromotion in TiNi SMA group can promote the healing of fracture.