RESUMO
A combined histochemical, biochemical and electrophoretic study with respect to the enzymes succnic dehydrogenase(SDH), myofibrillar adenosine triphosphatase (m-ATPase), lactate dehydrogenase (LDH) isozymes and myosin light chains was carried out to investigate the response of rat gastrocnemius muscle (medial head). Twelve weeks after thyroidectomy, the results indicated a shift from fast to slow type pattern of LDH isozymes, fibre type transformation from Type II to Type I and a decrease in SDH and m-ATPase activity. The results suggest, possible thyroidal involvement in determining the phenotypic properties of skeletal muscle.
Assuntos
Adenosina Trifosfatases/análise , Animais , Hipotireoidismo/enzimologia , Isoenzimas/análise , L-Lactato Desidrogenase/análise , Masculino , Fibras Musculares de Contração Rápida/enzimologia , Fibras Musculares de Contração Lenta/enzimologia , Proteínas Musculares/análise , Cadeias Leves de Miosina/análise , Ratos , Succinato Desidrogenase/análise , Tireoidectomia/efeitos adversosRESUMO
The present study analyzes the regeneration of skeletal muscle in streptozotocin (STZ) induced diabetic rats. Two weeks later the gastrocnemius muscle from diabetic rats were transplanted into diabetic and normal host to initiate regeneration and the normal gastrocnemius muscle was transplanted in normal and diabetic hosts for comparison. The regenerates were analyzed after 15 and 30 days of transplantation for histochemical (with respect to SDH and m-ATPase) and 7, 14, 21 and 28 days after transplantation for biochemical studies (with respect to SDH and m-ATPase). Least enzymatic activity and the poorest regenerative ability in case of normal muscle in diabetic host (NM-DbH) and comparatively higher enzymatic activity and better regenerative ability in diabetic muscle in normal host (DbM-NH) was observed. The result of this study strongly supports that the normal host environment is crucial for the muscle recovery.