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1.
Braz. j. microbiol ; Braz. j. microbiol;44(4): 1155-1161, Oct.-Dec. 2013. graf, tab
Artigo em Inglês | LILACS | ID: lil-705275

RESUMO

An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers' intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.


Assuntos
Enterotoxinas/genética , Frutas/microbiologia , Staphylococcus aureus/isolamento & purificação , Argentina , DNA Bacteriano/genética , DNA Ribossômico/genética , Reação em Cadeia da Polimerase Multiplex , /genética , Staphylococcus aureus/classificação , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo
8.
Rev. argent. microbiol ; Rev. argent. microbiol;20(4): 195-9, oct.-dic. 1988. tab
Artigo em Espanhol | LILACS | ID: lil-79159

RESUMO

Mesófilos aerobios totales (MT), coliformes totales (CT), coliformes fecales (CF), Escherichia coli (EC), hongos y levaduras (HyL) y Salmonella fueron estudiados en 50 muestras de fideos frescos (32 a 35% de agua) preparados con huevo deshidratado o con huevo líquido. Los valores obtenidos fueron: (entre paréntesis el porcentaje de muestras positivas) MT, 10**4 a 10**6 UFC/g (48%); H y L, 10**2 UFC/g (76%); CT, 4 a 100/g (32%) y 460/g (2%); CF, 3 a 10/g (14%) y 21/g (6%). Para EC sólo dos muestras fueron positivas con 4 y 9/g respectivamente. Una sola serovariedad de salmonella (S. oranienburg) fue detectada en el 88% de las muestras


Assuntos
Enterobacteriaceae/isolamento & purificação , Microbiologia de Alimentos , Fungos/isolamento & purificação , Salmonella/isolamento & purificação , Leveduras/isolamento & purificação
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