RESUMO
The study was done to determine IgG and IgM antitetanus antibodies in mothers after antenatal immunization, and extent of transfer to the baby as measured by cord levels of tetanus specific IgG and IgM by ELISA using enzyme penicillinase and to study the effect of nutritional status of mothers on antibody response to tetanus toxoid and corresponding cord levels also to determine the number of doses required for protective antibody levels in cord blood. The results obtained in sera of 100 mothers at the time of delivery and their respective cord sera and results of 43 different control sera are discussed using ELISA technique.
Assuntos
Adulto , Ensaio de Imunoadsorção Enzimática , Feminino , Sangue Fetal/química , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Recém-Nascido , Troca Materno-Fetal , Estado Nutricional , Gravidez , Cuidado Pré-Natal , Tétano/imunologia , Toxoide Tetânico/administração & dosagem , VacinaçãoRESUMO
An Enzyme Linked Immunosorbent Assay (ELISA) was standardised to detect the presence of tubercule antigen in cerebrospinal fluids from patients with meningitis. CSF samples from clinically suspected cases of tuberculous meningitis, culture proven pyogenic meningitis and non-bacterial aseptic central nervous system (CNS) disorders were tested by ELISA to demonstrate its potential utility for routine diagnostic purpose. Tubercule antigen was detected in 73% cases of tuberculous meningitis and was absent in pyogenic and other non-bacterial CNS disorder cases. The test appears to be a promising approach for a definitive diagnosis of tuberculous meningitis.
Assuntos
Antígenos de Bactérias/análise , Diagnóstico Diferencial , Ensaio de Imunoadsorção Enzimática , Seguimentos , Humanos , Mycobacterium tuberculosis/imunologiaRESUMO
Serum IgA, secretory IgA and serum C3 were estimated in 22 patients of chronic bronchitis with acute exacerbation. These were compared with 22 normal controls. There was no significant difference in the parameters studied. However, all patients showed a significant change in the above parameters when divided into mild, moderate and severe categories depending on the chronicity of the disease. An inverse relationship between serum C3 and secretory IgA was observed.
Assuntos
Doença Aguda , Adulto , Bronquite/imunologia , Doença Crônica , Complemento C3/análise , Estudos de Avaliação como Assunto , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina A Secretora/análise , Masculino , Pessoa de Meia-Idade , Fatores de TempoRESUMO
We evaluate the triphenyl tetrazolium chloride (TTC) dye reduction test for quicker diagnosis of urinary tract infections for its sensitivity and reliability to detect significant bacteriuria. Of the 1400 urine samples tested 780 (55.7%) had significant bacteriuria. TTC dye test was positive in 678 (86.9%) of those with significant bacteriuria thereby showing its usefulness. The test is simple and cheap and can be carried out in field situations.
Assuntos
Bacteriúria/diagnóstico , Criança , Pré-Escolar , Corantes/diagnóstico , Reações Falso-Negativas , Reações Falso-Positivas , Humanos , Sais de Tetrazólio/diagnósticoRESUMO
In 49 patients of chronic bronchitis with acute exacerbation, serum antibody titres were estimated against the respective pathogen isolated and cultured from sputum of individual patients. Antibody titres to Klebsiella and Staphylococcus coagulase positive organisms were found in 28 and 25 patients respectively. Paired serum samples from 21 patients showed rising antibody titres in 17 of them, whereas the antibody titres fell in the remaining 4 patients. Notably, only 2 patients demonstrated a four fold rise or fall in the antibody titres. The significance of these findings is discussed.