Observation on the functional characteristics of multidrug-resistant Mycobacterium tuberculosis-infected macrophage model / 中华微生物学和免疫学杂志

Objective:To observe the characteristics of the phagocytosis and bactericidal function of multidrug-resistant Mycobacterium tuberculosis(MDR- Mtb)-infected macrophage model, and the changes of the immune response and metabolic function in the process of phagocytosis and bactericidal function, aiming to provide reference for studying the role and mechanism of macrophages in the occurrence and development of multidrug-resistant tuberculosis(MDR-TB). Methods:We established MDR- Mtb and H37Rv-infected macrophage models, and used the colony-forming unit (CFU), Magnetic Luminex ? Assay and Cholesterol Assay kit to observe the effects on phagocytosis and bactericidal function, the secretion of Th1(IL-12/23 p40, IL-27 and TNF-α) and Th2 cytokines (IL-6 and IL-10) and cholesterol metabolism. The data were analyzed by SPSS25.0 software. The data were expressed as Mean± SD and analyzed by t test or F test. P<0.05 was considered statistically significant. Results:(1) After MDR- Mtb-infected macrophages, the intracellular CFU gradually increased and reached the highest at 24 h, while the extracellular CFU gradually decreased and reached the lowest at 24 h. The intracellular CFU at 48 h was lower than that at 24 h, while the extracellular CFU was higher than that at 24 h ( P<0.05). Both intracellular and extracellular CFU at 48 h were close to those at 4 h ( P>0.05). The intracellular CFU was lower than the H37Rv group at 8-48 h, while the extracellular CFU was higher than the H37Rv group ( P<0.05). (2) The level of IL-12/23 p40, IL-27, TNF-α, IL-6 and IL-10 of MDR-TB group were higher than those of blank group ( P<0.05), but the level of TNF-α and IL-6 at 24 h and 48 h were higher than that at 4 h ( P<0.05). IL-12/23 p40 and TNF-α at 48 h and IL-6 at 24 h were lower than those of the H37Rv group, while IL-27 at 48 h was higher than that of the H37Rv group ( P<0.05). (3) The levels of cholesterol of MDR-TB group at 24 h and 48 h were lower than those of 4 h and blank group ( P<0.05), but the level of cholesterol was similar to the H37Rv group at any time ( P>0.05). (4) TNF-α reached the highest when the intracellular CFU reached the highest at 24 h, and IL-6 reached the highest when the intracellular CFU decreased at 48 h. With the decreasing of cholesterol expression, the intracellular CFU increased and then decreased. Conclusions:MDR- Mtb could induce the phagocytosis and bactericidal function of macrophages, increase the expression of Th1 and Th2 cytokines and promote the utilization and consumption of cholesterol, but this function was weaker than that of H37Rv strain.

Chrysin inhibits LPS-induced autophagy of chondrocytes through PI3K/AKT signaling pathway / 中国药理学通报

Aim To explore the effect of chrysin on chondrocyte autophagy in rat chondrocyte osteoarthritis model induced by lipopolysaccharide and its mechanism. Methods Normal articular cartilage cells of 10 SPF SD rats were isolated and cultured in vitro, and the autophagy of rat chondrocytes was induced by LPS. The experiment was divided into blank control group, LPS group, chrysin (CHR) group and LPS + CHR group, the activity of cells in each group was detected by CCK-8 method, the mitochondrial membrane potential of cells in each group was detected by Rhodaminel23, and the protein expression of PI3K, AKT, p-PI3K, p-AKT, Beclin-1 and LC3 II in cells of each group was detected by reactive oxygen species, Western blot method of DCFH-DA. Results Chrysin could inhibit the autophagy induced by LPS, especially when the concentration of chrysin was 10 mmol · L

The association of serum neuron-specific enolase with other disease markers in chronic obstructive pulmonary disease: a case-control study

Objective: The aim of the present study was to investigate the association between serum neuron-specific enolase [sNSE] levels and gender, age, body mass index [BMI] in patients with chronic obstructive pulmonary disease [COPD]

Methods: This case-control study was carried out among 182 participants in Jiangxi Provincial chest hospital, Nanchang, China, in 2017. One hundred and two patients diagnosed with COPD based on the Global Initiative for Chronic Obstructive Lung Disease [GOLD] grading classification and 80 Non-COPD participants were recruited. Multivariate logistic regression analysis was employed to examine whether or not sNSE and other indicators were independently associated with COPD

Results: Serum NSE levels were not significantly different between the two groups [P=0.08]. Whereas in COPD sub-groups, the levels of sNSE increased parallelly in a GOLD stage-dependent manner. There was a positive correlation between PH, P[O2], pack-years, FEV1and the presence of COPD, but there was no significant correlation between sNSE, P[CO2] and the presence of COPD

Conclusions: Serum NSE gradually increased with the severity of COPD and its change reflected changes in brain cells. PH, P[O2], pack-years and forced expiratory volume in one second [FEV1], were independent risk factors for COPD patients

Preparation of isotope dilution mass spectrometry standards based on glucose pulse / 生物工程学报

Isotope Dilution Mass Spectrometry (IDMS) is the most accurate method for high-throughput detection of intracellular metabolite concentrations, and the key is getting the corresponding fully uniformly(U) ¹³C-labeled metabolites to be measured. The conventional procedure for getting fully U ¹³C-labeled metabolites is through batch cultivation, but intracellular metabolites concentrations by this method are generally low. By applying U ¹³C-labeled glucose pulse, combined with fast sampling and quenching, mixture of fully U ¹³C-labeled intracellular metabolites was successfully extracted with higher concentration from Pichia pastoris G/DSEL fed with fully U ¹³C-labeled glucose as only carbon source. Quantitative results from liquid chromatography tandem mass spectrometry (LC-MS) and gas chromatography tandem mass spectrometry (GC-MS) show that concentrations of organic acids, sugar phosphates, amino acids and nucleotides were 2-10 folds higher than those without glucose pulse. Therefore, the glucose pulse method can efficiently improve the usage of fully U ¹³C-labeled glucose converting to ¹³C-labeled metabolites, and achieve the detection of intracellular metabolites with lower concentrate than the instrument detection limit.

Role of Diabetes Mellitus on Treatment Effects in Drug-susceptible Initial Pulmonary Tuberculosis Patients in China / 生物医学与环境科学（英文）

We assessed the role of diabetes mellitus (DM) on treatment effects in drug-susceptible initial pulmonary tuberculosis (PTB) patients. A prospective study was conducted in eight provinces of China from October 2008 to December 2010. We enrolled 1,313 confirmed drug-susceptible initial PTB patients, and all subjects received the treatment regimen (2H3R3E3Z3/4H3R3) as recommended by the national guidelines. Of the 1,313 PTB patients, 157 (11.9%) had DM; these patients had more sputum smear-positive rates at the end of the second month [adjusted odds ratios (aOR) 2.829, 95% confidence intervals (CI) 1.783-4.490], and higher treatment failure (aOR 2.120, 95% CI 1.565-3.477) and death rates (aOR 1.536, 95% CI 1.011-2.628). DM was a contributing factor for culture-positive rates at the end of the second month and treatment failure and death of PTB patients, thus playing an unfavorable role in treatment effects of PTB.

Diagnostic Value of Cerebrospinal Fluid T-SPOT.TB for Tuberculousis Meningitis in China / 生物医学与环境科学（英文）

The aim of this study was to evaluate the diagnostic value of the cerebrospinal fluid (CSF) T-SPOT.TB test for the diagnosis of TB meningitis (TBM). A retrospective analysis of 96 patients with manifested meningitis was conducted; T-SPOT.TB test was performed for diagnosing TBM to determine the diagnostic sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). A receiver operating characteristic (ROC) curve was also drawn to assess the diagnostic accuracy. The sensitivity, specificity, PPV, and NPV of CSF T-SPOT.TB test were 97.8%, 78.0%, 80.3%, and 97.5%, respectively, for 52 patients (54.2%) of the 96 enrolled patients. The area under the curve (AUC) was 0.910, and the sensitivities of CSF T-SPOT.TB for patients with stages I, II, and III of TBM were 96.7%, 97.2%, and 98.9%, respectively. CSF T-SPOT.TB test is a rapid and accurate diagnostic method with higher sensitivity and specificity for diagnosing TBM.

Meta-analysis for Clinical Efficacy of Ivabradine Treating the Patients With Chronic Heart Failure / 中国循环杂志

Objective: To systemically evaluate clinical efficacy of ivabradine (IVA) treating the patients with chronic heart failure (CHF). Methods: We searched PubMed, Conchrane Library, EMbase, CBM, CNKI and Wanfang database to enroll the random control trials of IVA treating CHF up to 2016-10. Meta-analysis was performed by RevMan 5.2 software. Results: A total of 12 English literature were finally enrolled which including 2 groups: IVA group, n=807 and Control group, n=800. Meta-analysis showed that compared with Control group, IVA group had increased LVEF (MD=2.68, 95％CI 1.85-3.50, P<0.00001), decreased left ventricular end-diastolic volume (MD=-5.78, 95％ CI -9.79 to -1.76, P=0.005), decreased left ventricular end-systolic volume (MD=-8.91, 95％ CI -11.23 to -6.59, P<0.00001) and reduced N-terminal pro-brain natriuretic peptide (MD=-109.22, 95％ CI -126.42 to -92.01, P<0.00001); while systolic blood pressure (MD=6.02, 95％CI -0.80 to 12.84, P=0.08) and diastolic blood pressure (MD=3.6495％ CI -0.48 to 7.77, P=0.08) were similar between 2 groups. Conclusion: IVA improved ventricular remodeling in CHF patients and had no obvious effect on blood pressure. The long-term effect and safety of IVA should be further confirmed by high quality random control trial and the result from evidence-based medicine.

Characterization and genomic sequencing of PS2, a lytic phage that infects Serratia marcescens clinical isolates / 中国人兽共患病学报

The present study focused on the characterization and genomic sequence of phage PS2 that infects Serratia marcescens clinical isolates.The morphology of phage PS2 was observed with electron microscope.The one-step growth curve,host range,and stability of PS2 were investigated.In addition,Phage DNA was extracted from the purified phage particles using a MiniBEST Viral RNA/DNA Extraction Kit.DNA sample was analyzed by digesting with restriction enzymes.The phage DNA was used for constructing the sequencing library.The library was sequenced on a MiSeqTM platform.The whole genome sequence was obtained by Velvet (version:1.2.08) assembling.Phage PS2 belongs to the Myoviridae family.The linear,circularly permuted,167 266-bp double-stranded DNA genome of PS2 has high similarities to T4-1ike phages.The phage DNA contains 41.7％ GC and 276 ORFs.PS2 exhibited a 21-minute latent period and 70 PFU per cell at burst size when the pathogenic S.marcescens strain S2 served as a host.Further investigation suggested that PS2 is stable in a wide pH range (pH5 to pH10) and at extreme temperatures (50 ℃ and 60 ℃) after incubation alone at different pHs and different temperatures,respectively.The paper focused on the isolation and identification of a novel lytic S.marcescens phage,the biological characteristics,the whole genome sequencing and the preliminary study of bioinformatics,which laid the foundation for deeply analysis to the phage therapy of multi-drug resistant bacteria and the phage biological information.

Meta-analysis for Clinical Efficacy of Ivabradine Treating the Patients With Chronic Heart Failure / 中国循环杂志

Objective: To systemically evaluate clinical efficacy of ivabradine (IVA) treating the patients with chronic heart failure (CHF). Methods: We searched PubMed, Conchrane Library, EMbase, CBM, CNKI and Wanfang database to enroll the random control trials of IVA treating CHF up to 2016-10. Meta-analysis was performed by RevMan 5.2 software. Results: A total of 12 English literature were finally enrolled which including 2 groups: IVA group, n=807 and Control group, n=800. Meta-analysis showed that compared with Control group, IVA group had increased LVEF (MD=2.68, 95％CI 1.85-3.50, P<0.00001), decreased left ventricular end-diastolic volume (MD=-5.78, 95％ CI -9.79 to -1.76, P=0.005), decreased left ventricular end-systolic volume (MD=-8.91, 95％ CI -11.23 to -6.59, P<0.00001) and reduced N-terminal pro-brain natriuretic peptide (MD=-109.22, 95％ CI -126.42 to -92.01, P<0.00001); while systolic blood pressure (MD=6.02, 95％CI -0.80 to 12.84, P=0.08) and diastolic blood pressure (MD=3.6495％ CI -0.48 to 7.77, P=0.08) were similar between 2 groups. Conclusion: IVA improved ventricular remodeling in CHF patients and had no obvious effect on blood pressure. The long-term effect and safety of IVA should be further confirmed by high quality random control trial and the result from evidence-based medicine.

Characterization and genomic sequencing of PS2, a lytic phage that infects Serratia marcescens clinical isolates / 中国人兽共患病学报

The present study focused on the characterization and genomic sequence of phage PS2 that infects Serratia marcescens clinical isolates.The morphology of phage PS2 was observed with electron microscope.The one-step growth curve,host range,and stability of PS2 were investigated.In addition,Phage DNA was extracted from the purified phage particles using a MiniBEST Viral RNA/DNA Extraction Kit.DNA sample was analyzed by digesting with restriction enzymes.The phage DNA was used for constructing the sequencing library.The library was sequenced on a MiSeqTM platform.The whole genome sequence was obtained by Velvet (version:1.2.08) assembling.Phage PS2 belongs to the Myoviridae family.The linear,circularly permuted,167 266-bp double-stranded DNA genome of PS2 has high similarities to T4-1ike phages.The phage DNA contains 41.7％ GC and 276 ORFs.PS2 exhibited a 21-minute latent period and 70 PFU per cell at burst size when the pathogenic S.marcescens strain S2 served as a host.Further investigation suggested that PS2 is stable in a wide pH range (pH5 to pH10) and at extreme temperatures (50 ℃ and 60 ℃) after incubation alone at different pHs and different temperatures,respectively.The paper focused on the isolation and identification of a novel lytic S.marcescens phage,the biological characteristics,the whole genome sequencing and the preliminary study of bioinformatics,which laid the foundation for deeply analysis to the phage therapy of multi-drug resistant bacteria and the phage biological information.

Injury characteristics and injury analysis in Beagle dogs after multi-cabin explosion / 解放军医学杂志

Objective To investigate the injury characteristics of Beagle dogs after warship multi cabin explosion.Methods Forty-eight adult male Beagle dogs were placed in the simulated blast-cabin and adjacent cabin (24 each),and ammunitions respectively containing 0.75kg TNT and 3.50kg TNT were then detonated in the blast-cabin.The survival situation,fluctuation of vital signs,morphological changes of organs and the incidence of various types of injury of the dogs were observed immediately after the explosion to 24h after injury,and the neurological functions score was performed.Results Twenty dogs died immediately after the explosion,and another 9 dogs died 24h after the explosion.The total mortality was 60.42％(29/48),and the mortalities in blastcabin and adjacent cabin were 79.17％(19/24) and 41.67％(10/24),respectively.The dog's skull was penetrated by bomb fragments,and congestion and bleeding were observed in brain tissue,lung,heart,stomach,bowels,liver and kidneys.Extremities fracture,soft tissue contusions,perforation and rupture were also checked out after explosion.The fatality rate of bomb fragment injury,blast injury and combined bomb fragment-blast injury was 27.59％(8/29),17.24％(5/29) and 55.17％(16/29),respectively.Conclusions The combined bomb fragment-blast injury show high incidence and make high fatality rate and serious injury.Combatants should effectually shield themselves with occluded objects as far as possible to avoid damage and reduce fatality.The key of early treatment is to treat the multiple injuries promptly.Intravenous fluid therapy should be practiced after hemodynamic monitoring.

Observations on the Efficacy of Intermittent Liver-nourishing and Mind-regulating Acupuncture in Preventive Treatment of Migraine / 上海针灸杂志

Objective To investigate the clinical efficacy of intermittent liver-nourishing and mind-regulating acupuncture in preventive treatment of migraine. Method One hundred and twenty migraine patients were randomly allocated to treatment and control groups, 60 cases each. The treatment group received intermittent liver-nourishing and mind-regulating acupuncture and the control group took flunarizine hydrochloride capsules orally. The migraine score, the visual analogue scale (VAS) score and the headache severity rating score were recorded in the subjects before treatment and at the end of treatment and the 4-week, 8-week, 12-week, 16-week and 20-week follow-ups. The Self-Rating Anxiety Scale (SAS) score and the Self-Rating Depression Scale (SDS) were recorded before and after treatment.Result After one course of treatment, the total efficacy rate was 98.3% in the treatment group and 89.5% in the control group; there was a statistically significant difference between the two groups (P<0.05). There was a statistically significant difference in the migraine score between before treatment and at the end of treatment in the two groups (P<0.05) and between the two groups at the same follow-ups (P<0.05). There was a statistically significant difference in the VAS score between before treatment and at the end of treatment in the two groups (P<0.05) and between the two groups at the same follow-ups (P<0.05). There were statistically significant differences in the SAS and SDS scores between before treatment and at the end of treatment in the two groups (P<0.05) and between the two groups after treatment (P<0.05).Conclusion Intermittent liver-nourishing and mind-regulating acupuncture has marked efficacy in preventive treatment of migraine. It can increase the total efficacy rate and reduce the number of relapses.

Genetic analysis of a pedigree affected with inherited thrombocytopenia caused by a novel mutation of MYH9 gene / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To study genetic mutations and clinical features of a pedigree affected with MYH9-related disorders from Guangxi.</p><p><b>METHODS</b>Blood platelets were counted with a hemocytometer. Blood smear was carried out to detect the inclusion body in peripheral blood neutrophils. DNA and mRNA samples were extracted from blood samples from the members of the pedigree. Fragments of the MYH9 gene were amplified with PCR and directly sequenced.</p><p><b>RESULTS</b>The affected individuals presented with a triad of giant platelets, decreased platelet count and inclusion bodies in the neutrophils with variable expressivity. A heterozygous deletional mutation (c.5803delG) in exon 41 of the MYH9 gene was found in all of the 8 affected individuals, which led to a frame-shift and change of 26 amino acids at the C-end of the tail domain of nonmuscle myosin heavy chain IIA (NMMHC-IIA) (p.Ala1935Profs*12). The same mutation was not found among healthy members of the pedigree.</p><p><b>CONCLUSION</b>The c.5803delG mutation probably underlies the MYH9-related disorders in this pedigree. The mutation has altered the C-end of the tail domain of the NMMHC-IIA protein, resulting in mild clinical symptoms in the affected individuals.</p>

Prevalence and Risk Factors Associated with Adverse Drug Reactions among Previously Treated Tuberculosis Patients in China / 生物医学与环境科学（英文）

We assessed the incidence of adverse drug reactions (ADRs) with anti-TB medications and evaluated the risk factors for developing ADRs in previously treated tuberculosis patients in China. All patients received the first-line anti-TB regimen (2HREZS/6HRE) as recommended by the national guidelines. Clinical and laboratory evaluations were performed once a month. Out of the 354 participants, 262 (74.0%) experienced ADRs such as hyperuricemia (65.0%, 230/354), hepatotoxicity (6.2%, 22/354) and hearing disturbances (4.8%, 17/354). ADRs were significantly associated with diabetes mellitus [OR (95% CI): 15.5 (2.07-115.87)]; however, weight more than 50 kg [OR (95% CI): 0.41 (0.22-0.85)] was a protective factor for occurrence of ADRs. Hyperuricemia is the most common adverse event but, most patients with hyperuricemia showed increased tolerance for high uric acid levels. Low body weight and diabetes mellitus increased the risk of the occurrence of ADRs during anti-TB treatment.

Risk of Treatment Failure in Patients with Drug-susceptible Pulmonary Tuberculosis in China / 生物医学与环境科学（英文）

The objective of this prospective study of the risks of treatment failure in patients with drug-susceptible pulmonary tuberculosis (PTB) was to provide reference data to help develop a disease control strategy. Participants were recruited in eight provinces of China from October 2008 to December 2010. A total of 1447 patients with drug-susceptible PTB and older than 15 years of age were enrolled. Demographic characteristics, bacteriological test results, and patient outcome, i.e., cure or treatment failure were recorded and compared using the chi-square or Fisher's exact tests. Multivariate logistic regression was used to identify factors associated with risk of treatment failure. Of the 1447 patients who were enrolled, 1349 patients (93.2%) were successfully treated and 98 (6.8%) failed treatment. Failure was significantly associated with age 365 years [odds ratio (OR)=2.522, 95% confidence interval (CI): (1.097-5.801)], retreatment [OR=2.365, 95% CI: (1.276-4.381)], missed medicine [OR=1.836, 95% CI: (1.020-3.306)], treatment not observed [OR=1.879 95% CI: (1.105-3.195)], and positive culture result after the first [OR=1.971, 95% CI: (1.080-3.597)] and second month [OR=4.659, 95% CI: (2.590-8.382)]. The risk factors associated with treatment failure were age 365 years, retreatment, missed medication, treatment not observed, and positive culture at the end of month 1 or month 2. These risk factors should be monitored during treatment and interventions carried out to reduce or prevent treatment failure and optimize treatment success.

Development of Tripterygium glycosides nano-carries based on "nanoemulsion-gels" and its pharmacodynamics / 中国中药杂志

The aim of this study is to develop the Tripterygium glycosides nanoemulsion gels and investigate its pharmacodynamics. Oleic acid was used as oil phase, polyoxyethylene castor oil as surfaetant, and 1,2-propanediol as cosurfactant to screen the formula of Tripterygium glycoside nanoemulsion using the pseudo-temary phase diagrams. Then the nanoemulsion gels was prepared. The ICR mouse ears were sensitazated by 7% DNCB, and then were excited by 0.3% DNCB to stimulate the model of mouse chronic dermatitis and eczema. The concentrations of IFN-γ, IL-4 and IL-8 in mouse blood were determined by ELISA. The results showed that Tripterygium glycosides nanoemulsion gels could significantly inhibit the swelling of mouse ears(P < 0.01) and ameliorate the edama and erythema of model mouse ears skin. Also it could significantly decrease the expression of IFN-γ and IL-4 in model mouse blood. Tripterygium glycosides nanoemulsion gels had a good therapeutic effect on mouse model of dermatitis and eczema. It was expected to provide a new and long-acting exterernal preparation for the treatment of dermatitis and eczema.

Prognostic value of circulating catestatin levels for in-hospital heart failure in patients with acute myocardial infarction / 中华心血管病杂志

<p><b>OBJECTIVE</b>To determine whether circulating level of catestatin (CST) could provide prognostic information independently of conventional risk markers for the development of in-hospital heart failure in patients with ST-segment elevation myocardial infarction (STEMI).</p><p><b>METHODS</b>The data of 120 STEMI patients (mean age: 61 years, 73% male) were collected from the Second Hospital of Shanxi Medical University and Taiyuan Central Hospital between November 2010 and September 2011.The patients were categorized into 4 groups according to CST (ng/L) quartile: ≤ 74.72, 74.73-79.67, 79.68 - 84.21 and ≥ 84.22 ng/L. Clinical features, therapeutic approaches were compared among groups. The patients were also grouped according to Killip class: Killip level I (n = 68), Killip level II (n = 23), Killip level III (n = 18), Killip level IV (n = 11). CST, NE and NT-proBNP were compared among groups. The Spearma rank correlation and multivariate logistic regression analysis were applied to determine the association between risk factors and in-hospital heart failure. Receiver-operator characteristic (ROC) curve was performed to evaluate the power of CST and NT-proBNP on predicting in-hospital heart failure.</p><p><b>RESULTS</b>Gender, hospital days, past history of smoking, hypertension, myocardial infarction, CK-MB peak level, TnI peak level, heart rate, blood pressure, blood glucose, blood lipid levels on admission and early reperfusion therapy were similar among groups. Patients with higher CST values were more likely to be older, to have lower body mass index, to have higher white blood cell count, CysC, hs-CRP, NE, NT-proBNP, past history of angina, diabetes mellitus, being diuretic users, and to have a lower ejection fraction (all P < 0.05). Higher CST levels were also associated with increased risk of heart failure (P < 0.05). In proportion with the deterioration of the cardiac function, CST, NE, NT-proBNP concentration gradually increased (all P < 0.05). Spearman rank correlation analysis showed that the CST was negatively correlated with LVEF (r(s) = -0.923, P < 0.001) and positively correlated with NT-proBNP (r(s) = 0.884, P < 0.001). After multivariate adjustment, CST remained to be an independent risk factor for the development of in-hospital heart failure (OR = 1.125, 95%CI: 1.056 - 1.198;P < 0.001). The area under the ROC curve of CST and NT-proBNP was 0.777 and 0.874. Using CST = 77.29 ng/L as a cut-off value, the sensitivity was 92.8% and specificity was 70.6% for predicting the development of in-hospital heart failure.</p><p><b>CONCLUSION</b>The plasma CST level is an independent predictor for the development of in-hospital heart failure in patients with STEMI.</p>

Mucocutaneous venous malformation in a Chinese family / 中华皮肤科杂志

ObjectiveTo analyze the clinicopathologic features of hereditary cutaneomucosal venous malformation (VMCM) in a Chinese family.MethodsFamily history was investigated in a family with VMCM,and tissue specimens were obtained from the lesions of the proband and subjected to histopathological analysis.ResultsAmong 65 members from 5 generations of the family,19 were affected by VMCM,hinting an autosomal dominant inheritance.None of the family members experienced gastrointestinal bleeding,central nervous system disorders,or cardiac defects.Affected individuals usually presented with multiple irregularly sized,blue-violet,elevated and slightly indurated masses located in the oral mucosa and subcutaneous tissue of the extremities.Pathological analysis showed malformed veins with abnormally dilated cavities and irregularly thickened walls.Although small veins were abnormally proliferating and clustered,there was no endothelial discontinuity.The smooth muscle layer was thickned in a varying degree or absent.ConclusionA diagnosis of VMCM is made according to the inheritance manner,clinical manifestation and pathological findings.

Biomechanical study and clinical choice of different fixation devices to treat femoral fracture / 医用生物力学

Objective To investigate the biomechanical properties of transverse fractured femurs when fixed by TiNi shapememory alloy embracing plate (TiNi SMA), limitedcontact dynamic compression plate (LCDCP) and static interlocking intramedullary nail (SIiN), respectively, so as to provide the theoretical evidence for orthopedic surgeons in clinical application. Methods Eight pairs of cadaveric femurs were used with one side randomly fixed by TiNi SMA and SIiN and the other side fixed by LC-DCP. Then the axial compression test, threepoint bending test, and torsion test were conducted. Results For axial compression test, the strains of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). The displacements of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). For three-point bending test, the displacements of TiNi SMA group were greater than those of LC-DCP group and SIiN group (P0.05). For torsion test, the torsion angels of TiNi SMA group were similar to those of SIiN group (P>0.05), but the torsion angles of LC-DCP group were smaller than those of TiNi SMA group（≥3 N•m）and SIiN group (P<0.05). Conclusions When the fractured femurs are fixed by three internal fixation devices, the stability of TiNi SMA group is weakest, SIiN group being better, and LCDCP group being best. The axial micromotion in TiNi SMA group can promote the healing of fracture.

Total laparoscopic radical trachelectomy with ascending branches of uterine arteries preservation / 中华医学杂志(英文版)

Radical trachelectomy (RT) is a fertility-sparing treatment for young women with early-stage cervical cancer. We report here a case of a 30-year-old nulliparous woman who presented with stage IA2 cervical squamous cancer. She was treated with total laparoscopic radical trachelectomy (TLRT) and laparoscopic pelvic lymphadenectomy (LPL). During this procedure, the ascending branches of uterine arteries were preserved. No metastasis was identified after fourteen months of follow-up. The menstrual pattern normalized and the patient has been attempting to conceive for two months. TLRT might be a safe fertility-preserving procedure for early-stage cervical cancer, due to its minimally invasive nature and shorter recovery time. However, more data are required on recurrence rate, fertility rate and pregnancy outcome in order to fully evaluate the therapeutic efficacy of TLRT.