RESUMO
To study the influence of different processing technigue in preparing allogeneic bone on proliferation rate and activity of rabbit osteoblasts in vitro, Osteoblasts were cultured combined with allogeneic bone prepared with different processing techniqne. Cell proliferation and activity were assessed 48 and 96h with MTT colorimetry and assay of alkaline phosphatase activity. The results showed that freezed bone (FB) and lyophilized bone (LB) inhibited cell growth and activity, while mineralized bone matrix (DBM),bone matrix gelatin (BMG), and autolyzed antigen extracted allogeneic bone(AAA) enhanced them. With the exception of FB and LB, the three others were biocompatible for osteoblasts.
RESUMO
The present study was conducted to investigate the combined effects of recombinant human bone morphogenetic protein 2 (rhBMP 2) and dexamethasone (Dex) on the proliferation and differentiation of bone marrow stromal cells (BMSC). BMSCs were subjected to the treatment with different concentrations of rhBMP 2, Dex, or a mixture of rhBMP 2 and Dex, then proliferation and differentiation of the attached cells were measured with methylthiazol tetrazolium (MTT) method, Coomassie brilliant blue assay and alkaline phosphatase (ALP) measurement kit. The results showed that with treatment of rhBMP 2 alone could promote the proliferation of BMSCs and upregulate the expression of ALP in BMSCs. Treatment with Dex alone could also augment the expression of ALP, whereas high concentration of Dex could inhibit the proliferation of BMSC. Moreover, significantly greater differentiation of BMSCs were found in combined treatment group compared with individual treatment groups. The above results suggested that rhBMP 2 and Dex could synergistically promote the proliferation of BMSC and its differentiation to osteoblast.