RESUMO
In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.
Assuntos
Animais , Masculino , Camundongos , Células Cultivadas , Imunoprecipitação da Cromatina , Regulação da Expressão Gênica , Genes Reporter , Hepatócitos/metabolismo , Fígado/metabolismo , Camundongos Endogâmicos ICR , Camundongos Transgênicos , Proteínas de Transporte de Monossacarídeos/biossíntese , Mutagênese Sítio-Dirigida , PPAR alfa/genética , PPAR gama/agonistas , Regiões Promotoras Genéticas , Isoformas de Proteínas/biossíntese , Elementos de Resposta , Tiazolidinedionas/farmacologiaRESUMO
<p><b>OBJECTIVE</b>To identify Cassia seeds of six species by capillary electrophoresis.</p><p><b>METHOD</b>The water-soluble extracts of Cassia seeds of six species were analyzed by capillary electrophoresis. The running buffer was 0.1 mol.L-1 borate, 0.1 mol.L-1 SDS, pH 8.5. The separation voltage was 25 kV.</p><p><b>RESULT</b>Four common peaks could be found in the electropherograms of six species Cassia seeds, and the characteristic peaks could also be observed.</p><p><b>CONCLUSION</b>Fingerprints of the six species of Cassia seeds show significant differences, which can be used for their identification.</p>