RESUMO
Hepatitis B virus (HBV)-human DNA integration is a commonly seen event in the evolutionary process of hepatocellular carcinoma (HCC). HBV chronic infection induces HBV mutation, HBV integration and the inflammatory microenvironment alternation in the hosts, which provides a evolutionary soil for the hepatocarcinogenesis. The second generation sequencing technique provides a better approach for understanding the HBV integration mechanism in HCC. This review focuses on the features of HBV integration and influences of HBV integration on human, virus genomes, and discusses the association of HBV integration with HCC.
RESUMO
<p><b>OBJECTIVE</b>To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.</p><p><b>METHODS</b>Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.</p><p><b>RESULTS</b>The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.</p><p><b>CONCLUSION</b>The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.</p>