Predictive value of C-reactive protein/albumin ratio and systemic immune-inflammation index for opportunistic infection among acquired immune deficiency syndrome patients / 中国医师进修杂志

Objective:To explore the predictive value of C-reactive protein/albumin ratio (CAR) and systemic immune-inflammation index (SII) for opportunistic infection among acquired immune deficiency syndrome (AIDS) patients.Methods:From March 2020 to March 2022, a total of 220 AIDS patients treated in Honghu Center for Disease Control and Prevention were enrolled and they were divided into opportunistic infection group (178 cases) and non-infection group (42 cases) according to whether infection occurred; they were also assigned to bacterial infection group (22 cases), fungal infection group (63 cases) and mixed infection group (93 cases) according to different etiological characteristics. The general data were collected and CAR, SII were calculated according to laboratory indicators. Logistic regression was used to screen the risk factors affecting opportunistic infections or different etiological infections. The predictive value of CAR and SII on opportunistic infection and different etiological infections were compared by using receiver operating characteristic (ROC) curve.Results:The CD 4+T cell count, lymphocyte count were lower in the opportunistic infection group and the CRP, CAR, white blood cell count, neutrophil count, SII were higher in the opportunistic infection group compared with non-infection group, there were statistical differences ( P<0.05). The results of Logistic regression analysis showed that CAR and SII were the independent risk factors of opportunistic infection ( P<0.05). The area under the curve (AUC) of CAR to predict opportunistic infection was 0.886(95% CI 0.837 - 0.925), the specificity and sensitivity was 97.0% and 69.1%; the AUC of SII to predict opportunistic infection was 0.743(95% CI 0.680 - 0.799), the specificity and sensitivity was 52.4% and 88.2%. The results of Logistic regression analysis showed that CAR, monocyte count and SII were the independent risk factors for bacterial infection ( P<0.05). CAR and SII were the independent risk factors for fungal infection ( P<0.05). CD 4+ T cell count, CAR and SII were the independent risk factors for mixed infection ( P<0.05). The AUC of CAR to predict bacterial, fungal and mixed infection were 0.898(95% CI 0.797 - 0.960), 0.828(95% CI 0.742 - 0.895) and 0.923(95% CI 0.864 - 0.962), the optimum critical value were 0.49, 0.43, 0.40, the specificity were 98.7%, 95.2% and 92.9%, the sensitivity were 72.7%, 66.7% and 80.6%. The AUC of SII to predict bacterial, fungal and mixed infections were 0.627(95% CI 0.497 - 0.744), 0.782 (95% CI 0.633 - 0.811) and 0.780(95% CI 0.700 - 0.847), the optimum critical value were 385.13, 379.27, 390.10, the specificity were 55.4%, 52.4% and 54.8%, the sensitivity were 77.3%, 85.7% and 90.3%. Conclusions:CAR and SII can be used as predictors of AIDS opportunistic infection.

Expressions of ZEB2 and C-myc in epithelial ovarian cancer and their clinical significance / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the expression patterns of ZEB2 and C-myc in epithelial ovarian cancer (EOC) and the associations between their expressions and the pathological features of EOC.</p><p><b>METHODS</b>The expressions of ZEB2 and C-myc proteins were detected immunohistochemically in 191 cervical cancer tissues and 13 normal ovarian tissues. The relationship between ZEB2 and C-myc protein expressions and the clinicopathological features of EOC was evaluated.</p><p><b>RESULTS</b>ZEB2 positive expression ratea in EOC tissues and normal ovarian tissues were 49.2% (94/191) and 30.8% (4/13), respectively (P=0.007), and C-myc positive expression rates in the two tissues were 53.9% (103/191) and 15.4% (2/13), respectively (P=0.001). A high expression of ZEB2 was positively correlated with the pathological type of the tumor (P=0.003), FIGO stage (P=0.028), T stage (P=0.002), and N stage (P=0.04), and a high expression of C-myc was positively correlated with FIGO stage (P=0.035), histological grade (P=0.039), and T stage (P=0.002). C-myc and ZEB2 expressions were positively correlated in EOC (P<0.001), and their co-expression in EOC was significantly correlated with T stage (R=0.358, P<0.001) and FIGO stage (P=0.008).</p><p><b>CONCLUSION</b>ZEB2 and C-myc can promote the progression, invasion and metastasis of EOC, and their combined detection may assist in early diagnosis of EOC.</p>

Measurement of Rho-kinase and CD4+CD25+ regulatory T cells in the peripheral blood in asthmatic patients / 中南大学学报(医学版)

OBJECTIVE@#To determine the levels of Rho-kinase and CD4+CD25+ regulatory T cells in patients with asthma, and the relationship between Rho-kinase and CD4+CD25+ regulatory T cells.@*METHODS@#We included 16 patients with moderate to severe asthma in the research group and 14 healthy people as the control group. The levels of Rho-kinase in the 2 groups were measured by ELISA. The level of CD4+CD25+ regulatory T cells in the 2 groups was measured by flow cytometry. The pulmonary function was measured by spirometer.@*RESULTS@#The level of Rho-kinase in the research group was higher than that in the healthy controls (P0.05). The level of CD4+CD25+ regulatory T cells in the peripheral blood of the 2 groups showed a positive correlation with FEV1% (r=0.380, P=0.038). There was no correlation between the level of Rho-kinase and the level of CD4+CD25+ regulatory T cells in the peripheral blood of the 2 groups (r=-0.438, P>0.05).@*CONCLUSION@#Rho-kinase and CD4+CD25+ regulatory T cells may play a key role in the pathogenesis of asthma.