Transcriptional Regulation between CCAT2 and AR in Prostate Cancer Cell Lines / 中国生物化学与分子生物学报

AR (androgen receptor) and CCAT2 are two prostate cancer (PCa)-related genes whereas their relationship is not yet reported. AR is the classical major functional gene in PCa progression. CCAT2, a non-coding gene, was identified based on big-data GWAS (Genome-Wide Association Studies) in the year of 2013. Androgen deprivation therapy (ADT) is usually used to treat PCa in the early stage. After persistent androgen deprivation, PCa would generally lead to castration resistant prostate cancer (CRPC), whereas the mechanism is yet unclear. Here we explore the function of AR and CCAT2 in PCa progression, especially their relation in androgen sensitive and insensitive cell model LNCap and DU145. We found a loop between AR and CCAT2 transcription by over-expression and knock-down strategies. In DU145 cells, G-CCAT2 activated AR mRNA level 2. 6 times, while T-CCAT2 inhibited it to 0. 2 times (P<0. 05). In LNCaP cells, G-CCAT2 could activate AR mRNA levels 1. 5 times, and TCCAT2 had no significant effect (P<0. 05). Under overexpression of AR in DU145 cells, the expression of CCAT2 increased 2. 9 times (P < 0. 05). The abundance of CCAT2 decreased to 0. 48 (P < 0. 05) in LNCaP cells by AR knock-down. Reporter gene analysis showed that CCAT2 could function on the AR promoter. We then performed CCK8 assays and AR protein level detection as supplement for the new gene CCAT2 studies. Finally we primarily studied some target genes that are related to AR and CCAT2 . The results showed that the G-CCAT2 transcript could activate AR expression in LNCap cells while UCCAT2 had no significant effect. In DU145 cells, G-CCAT2 exhibited a more relative stronger activation effect on AR, and U-CCAT2 could inhibit AR transcription. AR activates the transcriptional activity of CCAT2 in both cell lines, suggesting a feedback regulation between them. Our data showed that there would be a feedback loop between CCAT2 and AR, which may indicate a new method for PCa treatment.

Effect of Vitamin D3 to Platelet Activation Mediated by Tumor Cell Culture Medium / 中国实验血液学杂志

OBJECTIVE@#To investigate the effect of vitamin D3 to platelet activation by tumor cell culture medium.@*METHODS@#The peripheral blood platelets of BALB/c mice were isolated. The platelets were activated in 4T1 culture fluid for 24 h. The platelets were divided into 7 groups: control group, activation group, 1 nmol/L vitamin D3 group, 10 nmol/L vitamin D3 group, 50 nmol/L vitamin D3 group, 100 nmol/L vitamin D3 group, and positive drug (0.1 μmol/L eptifibatide) group. CCK-8 assay was used to detect the platelet proliferation at 24, 48 and 72 h. Flow cytometry was used to detect the expression of CD61 and CD62p and receptor for advanced glycation end products (RAGE) at 24, 48 and 72 h. ELISA was used to detect the level of platelet-endothelial cell adhesion molecule-1 (PECAM-1) at 24, 48 and 72 h.@*RESULTS@#The CD41@*CONCLUSION@#Vitamin D3 shows antiplatelet effect and can inhibit platelet proliferation and activation.

Detection of HHV-8 Virus in 450 Free Blood Donons in Wuhan and Its Genotyping Based on ORFK1 Gene / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the infection of HHV-8 virus in free blood donors in Wuhan.</p><p><b>METHODS</b>Whole blood samples were collected from 450 free blood donors in Wuhan, and the genomic DNA extraction and serum collection were performed respectively. And then, the positive rate of HHV-8 was detected by PCR and ELISA, the positive detection rates were compared between them. Finally, HHV-8 ORFK1 gene was cloned by PCR in the positive samples, and the HHV-8 ORFK1 gene th was genetyped through sequencing analysis, homology comparison and phylogenetic tree construction.</p><p><b>RESULTS</b>25 and 23 cases of positive samples were detected by PCR and ELISA, their positive rate were 5.56% and 5.11% respectively, and without statistically significant difference using χ test analysis (P > 0.05). Based on the results of ORFK1 gene cloning and sequence analysis in 23 positive samples, 15 samples C subtype of had HHV-8 ORFK1 gene, and 8 cases had A subtype had HHV-8 ORFK1 gene.</p><p><b>CONCLUSION</b>There is a certain percentage of HHV-8 infection in the free blood donors in Wuhan. It is suggested to increase the HHV-8 virus screening for free blood donors, so as to ensure the quality of blood.</p>

An analysis on health life expectancy of adult residents in Zhejiang Province / 预防医学

Objective To analyze the health life expectancy (HALE) of the residents aged 15 years and above in Zhejiang Province and to evaluate the health level among adults. Methods The study was based the mortality data collected from Zhejiang Chronic Disease Surveillance Information and Management System, and the mortality rates were from the Under-Reporting Survey and the sample data in Zhejiang from the 5th National Health Service Survey in 2013. A Sullivan' s method was used to calculate the HALE. Results The life expectancy and self-evaluated HALE were 63.62 years, 55.80 years, respectively, and the difference was 7.82 years (61.49 years VS 55.13 years in males and 66.10 years VS 56.57 years in females) . The self-evaluated HALE 55.89 years in urban and 55.54 years in rural residents aged 15 years and above. The proportion of self-evaluated HALE in the total life expectancy was 87.70%, and it decreased with age, and the proportion has dropped to 67.31% in ≥65 age group. Life expectancy without chronic morbidity was 46.68 years (46.35 years in males and 47.01 years in females; 44.03 years in urban and 47.15 years in rural areas) among residents aged 15 years and above, and the proportion of self-evaluated HALE without chronic morbidity in the total life expectancy without chronic morbidity was 73.37%. Conclusion In general the proportion of self-evaluated HALE in the total life expectancy was small , and males had a greater proportion than female, and adults in rural areas had a greater proportion than that in urban areas. We should consider NCDS management for the elderly residents and to improve the life quality among the elderly residents.

The impact of ambient particulate matter (PM10) on the population mortality for cerebrovascular diseases—a case-crossover study / 中华流行病学杂志

Objective To analyze the association between the concentration of ambient inhalable particulate matter (PM10) and population mortality for cerebrovascular diseases and to explore the impact of PM10 on cerebrovascular diseases.Methods Data including meteorological factors,air pollutants (NO2,SO2 and PM10) and cerebrovascular disease mortality in one district of Beijing from 2004 to 2008 were collected and both symmetric bidirectional case-crossover design and conditional logistic regression model were used to analyze the associations among them.Results After adjusting the influence of meteorological factors as daily average temperature and relative humidity,the single pollutant model showed that there was no significant lag effect.In the multipollutant model,the effect of the every 105.43 μg/m3 increase of ambient PM10 had a larger impact on the daily death of the cerebrovascular diseases with statistically significant difference (P＜0.05).The effect of ambient PM10 pollution on daily death of cerebrovascular diseases was significant for females,65 year-olds and in winter season.Conclusion Our data showed that elevated levels of ambient PM10was positively associated with the increase of cerebrovascular disease mortality.The elevated levels of ambient PM10 could lead to thc increase of the daily mortality on cerebrovascular diseases for females,elderly who were 65 or older and in winter seasons.

Assessment on analgesic and anti-inflammatory effects of Tongjingbao optimal formula and analysis on its active components / 中国中药杂志

<p><b>OBJECTIVE</b>To assess the analgesic and anti-inflammatory effects of Tongjingbao optimal formula and analyze its active components.</p><p><b>METHOD</b>Animals were divided into the model group, the Tongjingbao granule group and the Tongjingbao optimal formula group. The mice dysmenorrhea model was induced by oxytocin, and their content of blood calcium and MDA, NO, PGE2 in uterus were determined to assess the analgesic and anti-inflammatory effects of different components in Tongjingbao optimal formula and their impacts.</p><p><b>RESULT</b>All components of Tongjingbao optimal formula could extend the dysmenorrhea incubation period of mice with dysmenorrhea, reduce their average writhing time, increase the writhing inhibition rate, lessen the content of blood calcium and MDA, PGE2 in uterus, and enhance the content of NO in uterus.</p><p><b>CONCLUSION</b>All components of Tongjingbao optimal formula have the analgesic and anti-inflammatory effects, and different components show a synergistic effect in treating dysmenorrheal in many links.</p>

Progress of animal embryo technology in China / 现代生物医学进展

Embryo technology, including super-ovulation, embryo transfer and embryo nucleus transfer, was a well-developed technology in 20th century. It has been widely used in animal science and veterinary and boomed livestock industry in China. Chinese livestock producer could gain high grade breeds in short time through embryo technology by the reason that embryo technology could make outstanding gene spread out in herd. With the help of embryo technology, rare animals could have a chance to extend their progeny in fast changing world. In this article, we briefly introduced the process of embryo transfer and application of embryo technology in China.

Progress of animal embryo technology in China / 现代生物医学进展

Embryo technology, including super-ovulation, embryo transfer and embryo nucleus transfer, was a well-developed technology in 20th century. It has been widely used in animal science and veterinary and boomed livestock industry in China. Chinese livestock producer could gain high grade breeds in short time through embryo technology by the reason that embryo technology could make outstanding gene spread out in herd. With the help of embryo technology, rare animals could have a chance to extend their progeny in fast changing world. In this article, we briefly introduced the process of embryo transfer and application of embryo technology in China.