RESUMO
To study the correlation of four properties of traditional Chinese medicine and the function of reversing multidrug resistance (MDR) of tumor cells, with 580 herbs in Chinese Pharmacopoeia 2015 version as the research objects. CNKI, CBA, Wanfang, VIP, and PubMed were searched to screen the documents related to the reversal of MDR for collection, summarizing and analysis. The results of the research showed that a total of 114 species Chinese herbs had been reported to be associated with reversal of MDR in tumor cells. Among 15 Chinese herbs with heat nature, 7 herbs had the function of reversing MDR in tumor cells, accounting for 46.7%. Among the 48 herbs with cool nature, 12 herbs had the function of reversing MDR, accounting for 25%. Among the 211 herbs with cold nature, 46 herbs had the function of reversing MDR, accounting for 21.8%. Among the 179 herbs with warm nature, 34 herbs had the function of reversing MDR, accounting for 19%. Among the 127 herbs with neutral nature, 15 herbs had the function of reversing MDR, accounting for 11.8%. Through the analysis on the relationship between four properties of 114 kinds of traditional Chinese medicines and reversing multidrug resistance of tumor cells, this paper speculated that there was a certain correlation between four properties of traditional Chinese medicine and the function of reversing multidrug resistance of tumor cells.
RESUMO
The study was aimed to establish a liquid chromatography-tandem mass spectrometric method for the determination of the docetaxel concentration in rat plasma, and study the effect of coumarin constituents (imperatorin, isoimperatorin and oxypeucedanin) in Angelica dahurica on pharmacokinetics of docetaxel.Plasma was precipitated with acetonitrile and determined by LC-MS method with Paclitaxel as an internal standard. The specificity, linearity, range, accuracy, precision and stability of the method were suitable for the determination of docetaxel in plasma.Six sprague-dawley rats in each group received intragastric administration of docetaxel (50 mg•kg⁻¹), oxypeucedanin (8 mg•kg⁻¹) combined with docetaxel (50 mg•kg⁻¹), imperatorin (15 mg•kg⁻¹) combined with docetaxel (50 mg•kg⁻¹), and isoimperatorin(15 mg•kg⁻¹) combined with docetaxel (50 mg•kg⁻¹).Their drug plasma concentration was determined by LC-MS with Paclitaxel as an internal standard to draw plasma concentration-time curve, and the phamacokinetic parameters were calculated by DAS 2.0. The results showed that the phamacokinetic parameters of docetaxel all had notable changes when combined with imperatorin, isoimperatorin, and oxypeucedanin, respectively. The phamacokinetic parameters AUC and Cmax were significantly increased, indicating that coumarin constituents in Angelica dahurica could promote the oral bioavailability of docetaxel, and their effects were in the following order: oxypeucedanin> isoimperatorin> imperatorin.
RESUMO
Objective: To study the effects of 10 kinds of furanocoumarines in Angelica dahurica (oxypeucedanin, isoimperatorin, phellopyerin, isoimperatorin, imperatorin, byakangelicol, angelicin, scopoletin, umbelliferone, and byakangelicine) on the intestinal absorption and transportation of vincristine. Methods: The apparent permeability coefficient (Papp) of vincristin was used as evaluated parameter, and Caco-2 (human colon cancer cell lines) cell monolayer was used as intestinal epithelial cell model to study the effects of 10 furanocoumarins on transportation of vincristine in Caco-2 cell monolayer. Results: There were different effects of the 10 furanocoumarins on transportation of vincristine. Oxypeucedanin, isoimperatorin, and imperatorin could improve the transport of vincristine in Caco-2 cell monolayer (P < 0.05), while isoimperatorin, byakangelicol, angelicin, umbelliferone, and byakangelicine could inhibit the transport of vincristine in Caco-2 cell monolayer (P < 0.01). However, phellopyerin and scopoletin have no effects on the transport of vincristine on Caco-2 cell. Conclusion: Different kinds of furanocoumarins have different effects on intestinal transport of vincristine, which may be related to the structure of furocoumarins and the functional group about their connection.