RESUMO
Adriamycin resistance in HCC seriously hinders the treatment of patients, it is necessary to investigate the mechanisms. Autophagy is involved in adriamycin resistance and JNK2 is related to autophagy. However, whether JNK2 inducing drug resistance though autophagy is unknown. GL-V9, a new synthesized flavonoid derivative, has been proved of its anti-tumor effects. The aim of the study is to explore the role of JNK2-related autophagy on adriamycin-induced drug resistance and the effects of GL-V9 on reversing adriamycin resistance. We concluded that JNK2 played an important role in drug resistance induced by adriamycin. The high expression of JNK2 activated protective autophagy in Hep G2-DOXR cells under non-stress condition, which protected cells from drug attacking. Furthermore, we found that GL-V9 reversed adriamycin resistance by blocking the JNK2-related protective autophagy in HCC.
RESUMO
Objective: To observe the types, prevalence of cardiac dyssynchrony in patients of chronic systolic heart failure (SHF) , and to investigate the value of QRS duration >135 ms in selecting patients for cardiac resynchronization therapy (CRT). Methods: Forty-five patients with SHF were randomly divided into two groups according to QRS width: Group 1 (QRS width 135 ms could finely predict the presence of interventricular dyssynchrony, with a sensitivity of 80% and a specificity of 87. 5% ; while the same cutoff value to predict intraventricular dyssynchrony only yielded a sensitivity of 44. 1 % and specificity of 73. 6%. Conclusion: Intraventricular dyssynchrony and(or) interventricular dyssynchrony has a high prevalence in patients with SHF. A QRS duration cutoff value higher 135 ms can well evaluate the cardiac mechanical dyssynchrony in clinical practice, which may be of value for optimizing selection of CRT candidates and reducing the nonresponders.