Triterpene Glycosides from Sea Cucumber Holothuria scabra with Cytotoxic Activity / 中草药·英文版

Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity.Methods Triterpene glycosides from H.scabra were separated and purified by chromatography on DA-101,silica gel,and reversed-phase silica gel column,as well as RP-HPLC.Their structures were elucidated on the basis of spectral data and chemical evidence.Results Three triterpene glycosides were identified as scabraside D (1),fuscocineroside C (2),and 24-dehydroechinoside A (3).Their inhibition on P-388,A549,MKN-28,HCT116,and MCF-7 cells were significant.Conclusion Scabraside D (1) is a new triterpene glycoside,and compounds 2 and 3 are isolated from H.scabra for the first time.The glycosides 1-3 show the in vitro cytotoxicity against five human tumor cell lines in comparison to 10-hydroxycamptothecin.

Studies on antitumor activities of triterpene glycoside colochiroside A from sea cucumber Colochirus anceps / 中国中药杂志

<p><b>OBJECTIVE</b>To study the antitumor activities of the triterpene glycoside colochiroside A (CA) from the sea cucumber Colochirus anceps.</p><p><b>METHOD</b>The tests of antitumor activities in vitro and in vivo were applied to demonstrate the effect of CA.</p><p><b>RESULT</b>The preliminary cytotoxic assay of CA exhibited significant cytotoxic activity against 6 types cultured tumor cell lines of p388, HL60, A-549, SpC-A4, MKN-28, and SGC-7901, the mean of IC50 were (3.61 +/- 0.55) mg x L(-1). The preliminary antitumor assay of CA indicated that this saponin exhibited high inhibiting activity against the H22 live cancer and the S180 sarcoma cells in mouse. The inhibition ratio to H22 liver cancer were 34.8%, 43.9% and 52.2%, while the ratio to S180 sarcoma were 36.4%, 70.0%, the immunoregulatory founction study indicated CA has not significant effect on the developments of thymus and spleen.</p><p><b>CONCLUSION</b>The saponin CA exhibited remarkable antineoplastic activities in vitro and in vivo, and could not reduce the immunoregulatory founction of mice.</p>

A new triterpene glycoside from the sea cucumber Colochirous anceps / 中国海洋药物

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

STUDIES ON THE CHEMICAL CONSTITUENTS OF UNCARIA YUNANENSIS HSIA.C.C / 药学学报

AIM　To research the chemical constituents from dried roots of Uncaria yunanensis Hsia.C.C. METHODS　Modern chromatography was used to isolate chemical components. Their structure were identified by spectral analysis. RESULTS　Seven compounds were isolated and identified as 3β,6β,19α-trihydroxyurs-12-en-28 oic acid (I), 23-nor-24-esomethylene-3β,6β-19α-trihydroxyurs-12-en-28 oic acid (II), 3-oxo-6β,19α-dihydroxyurs-12-en-28 oic acid (III), oleanic acid (IV), 5,7,3′,4′-tetrahydroxy-flavan-3-ol (V), β-yohimbine (VI) and diangoutengjian I (VII). CONCLUSION　All of the above compounds were isolated for the first time from the root of this plant. Among them, compound VII is a new one.

Effects of esculentoside A on production of IL-1 and TNF by rabbit synovial cells / 第二军医大学学报

Objective: To investigate the influence of esculentoside A（EsA） on production of IL-1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL-1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5-40 μg/ml could significantly inhibit the production of IL-1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL-1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.

Progress in the studies on asterosaponins from starfish / 中国海洋药物

In this paper, a review and prospect to the progress in the studies on the chemical structures, pharmacological activities, methods of isolation and structural elucidation of as-terosaponins from starfish were reported.

Studies on chemical constituents from the sea cucumber Holothuria impatiens / 中国海洋药物

Objective To investigate the chemical constituents of the sea cucumber Holothuria impatiens collected from the South China Sea.Methods The pure compounds were isolated by extraction,partition and multi-chromatography.Their structures were elucidated on the basis of spectral analysis and chemical evidence.Results Three triterpene glycosides were isolated and identified as holothurin A1(Ⅰ),holothurin A(Ⅱ) and pervicoside C(Ⅲ),together with a physiological substance of adenosine(Ⅳ).Conclusion All were isolated from Holothuria impatiens for the first time.Three glycosides showed significant cytotoxic activities.

Progress in the Study of Marine Steroids / 中国海洋药物

Many marine steroids with novel structures have been isolated, some of which possess various bioactivities. The new compounds reported through 1996~2000 were reviewed in this article.

STUDIES ON THE ANTINEOPLASTIC CONSTITUENTS FROM MARINE BRYOZOAN BUGULA NERITINA IN THE SOUTH CHINA SEA( I ) / 中国海洋药物

Three active compounds were isolated from the marine bryozoan Bugula neritina living in the South China Sea by bioassay-guiding isolation method with a combination of extraction with suitable solvent and multiple column chromatography(Sephadex LH-20, ODS and preparative HPLC). Their structures were assigned as known bryostatin 4, bryostatin 5 and bryostatin 6 by intensive analysis of the data of high resolution 2DNMR(600 MHz,DQF-COSY,TOCSY,HMQC and ROESY). All these compounds were obtained from this bryozoan in the South China Sea for the first time and showed significant antineoplastic activities in vitro.

Studies on the Chemical Constituents of Common Leafflower(Phyllanthus urinaria) / 中草药

Six compounds were isolated from the whole herb of Phyllanthus urinaria L. They were id entified as ellagic acid (Ⅰ), 3, 3', 4-tri?O-methylellagic acid (Ⅱ), succinic acid (Ⅲ ), feru lic (Ⅳ ), ?-sitosterol-glucoside (Ⅴ ) and gallic acid (Ⅵ ) by means of spectral analysis and chemical reaction- Compound Ⅱ, Ⅲ, Ⅳ, and Ⅴwere isolated for the first time from this plant

Recent progress in the study of some chemical constituents from marine brown algae / 中国海洋药物

Many constituents with novel structures have been isolated from marine brown algae,some of which possess various bioactivities.The terpenoids,lipids,steroids,phlorotannins and some other compounds isolated from brown algae through 1993~2000 were reviewed in this article.

Studies on antineoplastic constituents from marine bryozoan bugula neritina inhabiting south china sea (IV):Isolation and structural elucidation of bryostatins 8 and 16 / 中国海洋药物

Eight active compounds were isolated from Bugula neritina Linnaeus collected off Dapen Bay in Guandong province, guided by P 388 bioassay system by a combination of successive chromatographies and preparative HPLC. By intensive analysis of the data of high resolution 2D NMR spectrum and ESI MS, their structures were elucidated as known bryostatins--bryostatin 4, 5, 6, 8, 10, 11, 16 and 18, among which bryostatin 8 and 16 were for the first time obtained from this animal in South China Sea and they showed significant antineoplastic activities in vitro.

Studies on antineoplastic constituents from chinese marine mollusc sea hare(Ⅰ) / 中国海洋药物

Two active compounds were isolated from chinese marine mollusc sea hare (Notarchus leachii cirrosus) by bioassay guided method with conidia of Pyricularia oryzae , and by a combination of extraction and purification with suitable solvent as well as multiple column chromatographies. Their structures were elucidated as (7 E) 1 acetoxy 8 chloro 7 (dichloromethyl) 3 methyoct 7 en 4 one (Ⅰ), (7 Z) 1 acetoxy 8 chloro 7 (dichloromethy l) 3 methyoct 7 en 4 one (Ⅱ). All compounds were obtained for the first time from this specimen and showed significant antineoplastic activities in vitro .

Effect of esculentoside A on apoptosis of mouse thymocyte / 第二军医大学学报

Objective:To investigate the effect of esculentoside (EsA) on apoptosis of murine thymocyte. Methods:Using electronic microscope, DNA agarose electrophoresis and flow cytometry analysis,the effect of EsA on apoptosis of murine thymocyte was examined. Results:The result showed that apoptosis of activated thymocyte by ConA was markedly promoted by 2.5, 5, 10 ?g/ml EsA in murine thymocyte culture for 3 h, but the spontaneous apoptosis was not affected by EsA. Conclusion:The results suggest that EsA has the positive effect on apoptosis of activated murine thymocyte.

Isolation and Identification of Antimutagenic Constituents in Hotbed Chives / 第二军医大学学报

Fresh hotbed chives solutions were extracted respectively by ether, acetic ether and n-butyl alcohol and protein constituents and non-protein constituents were obtained. Ether extractions (non-protein constituents) had the strongest antirriutagenic effects by the SOS chromotest Non-protein constituents of hotbed chives were analysed further by gas chromatography-mass spectrum and 30 chemical substances were isolated and identified. Their antimutagenic substances may be dimethyl disulfide, 4-methyl-2-pyridinethione, di-2-propenyl trisulfide, 3-(allylthio)-propionic acid.

STUDIES ON THE ACTIVE PRINCIPLES OF PHYTOLACCA ESCULENTA VAN HOUTTE I. THE ISOLATION AND IDENTIFICATION OF TRSTERPENOID SAPONINS / 第二军医大学学报

From the roots of phytolacca esculenta, six triterpenoid saponins were isolated. On the basis of spectral analysis and chemical properties, four of them were identified as 3-O-(?-D-glucopyranosyl-?-D-xylopyranosyl)-jaligonic acid-30-methyl ester, 3-O-?-D-xylo pyranosyl-jaligonic acid-30-methyl ester, 3-O- (?-D-glucopyranosyl-?-D-xylopyranosyl) -esculentic acid-30-methyl ester and 3-O-?-D-glucopyranosyl-jaligonic acid -30-methyl ester,and in sequence tentatively named as esculentoside"Jia""yi""bing'' and"ding" respectively.Pharmacological experiments showed that the total saponins and esculentoside "Jia" exert a considerable enhancement on phagocytic function of leucocytes and that the total saponins can promote DNA synthesis to make the DNA which was decreased by hydroxy-urea keep a normal level.

Using conidia of Pyricularia oryzae screening for bioactive portions from marine organisms / 第二军医大学学报

Objective:To screen for the bioactive marine organisms and to study the active constituents from them.Methods:Bioactivities of marine organisms extracts were screened by Pyricularia oryzae bioassay method and the constituents were isolated and identified by a combination of multi chromatography and spectral analysis.Results:Twenty ethanolic extracts and 5 aqueous extracts of the tested marine organisms showed activities causing morphological abnormality of P.oryzae mycelia.Fifty six compounds were obtained and identified from Sargassum carpophyllum,Ishige okamurai and Natarchus leachii freeri ,among which 28 compounds showed activities against P.oryzae and exhibited cytotoxicities on various cultured tumor cell lines,eight compounds showed inhibition effects on Fonsecaea pedrosoi in vitro .Conclusion:The screening data and bioactivity evaluation suggest the P.oryzae bioassay is suitable for preliminary screening of bioactive agents from marine organisms.