Association between polymorphisms in pigment epithelium-derived factor gene promoter region and non-alcoholic fatty liver disease in type 2 diabetes mellitus / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the association of serum pigment epithelium-derived factor (PEDF) level and polymorphisms in PEDF gene promoter region -358G→A with non-alcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes mellitus (T2DM) of Han Nationality in Fujian Province.</p><p><b>METHODS</b>A total of 282 T2DM patients with NAFLD (DM1 group) and 170 age- and gender-matched T2DM patients without NAFLD (DM2 group) were examined for PEDF gene SNP-358G→A polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum pigment epithelium-derived factor(PEDF) level, fasting plasma glucose (FPG), fasting insulin (FINS) and glycosylated hemoglobin (HbA1c) were also measured.</p><p><b>RESULTS</b>The patients in DM1 group showed a significantly higher mean level of serum PEDF than those in DM2 group (P<0.05). Logistic regression analysis revealed that PEDF level was an independent risk factor for NAFLD in T2DM. The frequencies of PEDF gene -358G→A genotypes (GG, GA, and AA) and alleles (G/A) differed significanly between DM1 and DM2 groups (P<0.05). In terms of PEDF gene SNP -358G→A alleles, the GA genotype carriers had a 2.032 times higher risk of developing NAFLD compared with the GG genotype carriers, and the risk increased to 2.068 times in the carriers of the A allele (GA and AA genotypes; P<0.05).</p><p><b>CONCLUSION</b>Serum PEDF level is an independent risk factor of NAFLD in T2DM. Elevated serum PEDF level is a protective factor against insulin resistance. In T2DM patients, PEDF gene promoter region -358G→A polymorphism is associated with NAFLD, and the A allele contributes to an increased risk of NAFLD.</p>

Association of gene polymorphism in promoter region of adiponectin gene and carotid artery intima-media thickness in patients with type 2 diabetes mellitus of Fujian / 中华内分泌代谢杂志

Objective To explore the association of-11377 site single nucleotide polymorphism in promoter region of adiponectin gene and carotid intima-media thickness(CIMT)in patients with type 2 diabetes mellitus.Methods The adiponectin gene-11377C→G polymorphism was identified by PCR-restriction fragment length polymorphism(RFLP)in 504 patients with type 2 diabetes mellitus(250 patients with increased CIMT and254 Datients with normal CIMT). Serum lipid and fasting plasma glucose were detected by full automatic biochemical analysor.fasting serum insulin(FINS) was measured by radioimmunoassay,and serum adiponectin level was assessed by ELISA.Results The frequencies of adiponectin gene-11377C→G genotype and allele were different between type 2 diabetic patients with normal and increased CIMT(P<O.01).In type 2 diabetic patients, the values of CIMT,diastolic blood pressure,low-density lipoprotein cholesterol (LDL-C)and homeostasis model assessment of insulin resistance (HOMA-IR) in-11377CC group were significantly lower than those in-11377CG +GG group(P<0.05 or P<O.01),but serum adiponectin level was higher in-11377CC group than that in -11377CG+GG group [(5.70 ±3.58 vs 4.72±2.34)mr/L,P<O.01].Variation at adiponectin gene-11377C→G was associated with increased CIMT in type 2 diabetic patients independent of serum adiponectin level(P= 0.037).Conclusion In type 2 diabetic patients of Fujian,the-11377C→G polymorphism of the adiponectin gene is associated with CIMT.

Therapeutic evaluation of gliclazide-MR on blood glucose excursion by self-monitoring of blood glucose in type 2 diabetic patients / 中华内分泌代谢杂志

A total of 123 type 2 diabetics was randomised into 3 groups to receive gliclazide-MR, gliclazide or glibenclamide treatments for 16 weeks.All the subjects took self-monitoring of blood glucose (SMBG) during the trial.The effect of therapy was similar in 3 groups.The subtraction value between maximum and minimum blood glucose, mean postprandial maximum blood glucose, postprandial 2 h blood glucose, postprandial 2 h serum insulin and hypoglycaemia events were lower in gliclazide-MR group than those in glibenclamide group (all P＜0.01).The data suggest that SMBG is an useful method to evaluate blood glucose excursion.

Endogenous subclinical hyperthyroidism: needs it to be intervened? / 中华内分泌代谢杂志

Objective To explore the effects of subclinical hyperthyroidism (SH) on heart and bone and the optionof treatment. Methods Forty-three patients with endogenous SH were divided into two groups by the TSH level: group A (21 cases,TSH 0.03-0.60 mIU/L) and group B (22 cases, TSH