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Objective:To explore the difference of constituents and contents of volatile oil in Perilla leaf and Perilla stem.Methods:The volatile oils of Perillaleaf and Perillastems were extracted with steam distillation method. Gas chromatography-mass spectrometry (GC-MS) technique was used to detect the chemical constituents of volatile oil from different parts of Perilla frutescens. The relative percentage content of each component was determined by peakarea normalization method, and the differences of the components and contents of volatile oils from different parts of Perilla frutescens were compared.Results:A total of 24 volatile oil components were identified in perilla leaves and 19 volatile oil components in Perilla stems. The most components in Perilla leaf and Perilla stems was 2-Hexanoyl furan. The content of 2-Hexanoyl furan in the oil of Perilla leaf reached 67.493%. The content of 2-Hexanoyl furan in the oil of Perilla stem reached 70.473%.Conclusions:The composition and content of volatile oil in different parts of Perillafrutescens are different.
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Objective:To explore the composition and content differences of volatile oil in the leaves of Phlomis umbrosa from different habitats, in order to clarify the chemical composition and content on efficacy.Methods:To collect the Phlomis umbrosa from Haoping area in Mei county and Sanyuan county of Shaanxi province. The volatile oil of Phlomis umbrosa was extracted by steam distillation method. Gas chromatography- mass spectrometry (GC-MS) was used to detect the chemical constituents of volatile oil.Results:Forty-nine kinds of volatile components were identified from the leaves collected in Haoping area of Mei county and fifty-six volatile component were identified from the leaves collected in Sanyuan county. There are five ingredients in common, excluding diacetone alcohol. 2-tert-butoxy-5-methylthiophene is the main volatile oil composition and the content reached 18.009% of leaves collected from Haoping area in Mei county, while paclitaxel is main volatile oil composition and the content reached 50.728% in Sanyuan county.Conclusions:The chemical compostion and content of volatile oil of Phlomis umbrosa in different habitats are different.
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<p><b>OBJECTIVE</b>To investigate the influence of Shi herb (Santalum album, SA) to the tissue distribution of danshensu (DSS) which is the main hydrosoluble component of Jun herb (Salvia miltiorrhiza, SM) in rabbits by HPLC.</p><p><b>METHOD</b>Rabbits were oral administrated decoction of SM and SM-SA, respectively. Perchloric acid (10%) was used to precipitate the tissue samples of rabbits heart, brain, liver, kidney, acetic ether was used to extracte supernatant, and the internal standard was p-hydroxybenzoic acid. The content of DSS of SM in tissues was detected.</p><p><b>RESULT</b>The content of DSS reached the highest point close to 50 min in the mentioned tissues. Before and after co-administration, the sequences of average concentration of DSS in tissues were C(kidney) > C(heart) > C(brain) > C(liver) and C(kidney) > C(liver) > C(brain) > C(heart) respectively. Compared with SM administrated singly, the content of DSS in every tissues of co-administration was higher.</p><p><b>CONCLUSION</b>In Danshenyin Formulae, SA can increase concentration of DSS in target tissues significantly, and therefore therapeutic effect of SM for cardiovascular disease is raised.</p>