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1.
China Pharmacy ; (12): 2037-2041, 2019.
Artigo em Chinês | WPRIM | ID: wpr-817177

RESUMO

OBJECTIVE: To prepare Magnolol nano-crystal suspension (MAG-NS), and to conduct quality evaluation. METHODS: The preparation technology of MAG-NS was optimized by central composite design-response surface methodology with OD value of particle size and polydispersity coefficient as evaluation indexes, using volume ratio of organic phase to water phase, ratio of excipient to drug, concentration of magnolol as factors and conduct validation tests. The quality of MAG-NS prepared optimal technology was evaluated. RESULTS: Optimized technology included that the volume ratio of organic phase to water phase was 1 ∶ 5, mass ratio of excipient to drug was 4 ∶ 1, concentration of magnolol was 2 mg/mL. In 3 times of validation tests, average OD value was 0.940 0 (RSD=0.08%), relative error of which to predicted value 0.977 7 was 3.86%. magnolol nano-crystals of MAG-NS prepared by the optimal technology were spherical, uniform in size, smooth in surface, with particle size of (34.88±0.33) nm, polydispersity coefficient of 0.032±0.001 and drug loading amount of (17.83±0.92)%. CONCLUSIONS: Established preparation method is simple and feasible. Prepared MAG-NS is in line with quality requirements. It can provide reference for further development and utilization of MAG-NS.

2.
Practical Oncology Journal ; (6): 346-350, 2016.
Artigo em Chinês | WPRIM | ID: wpr-499369

RESUMO

Objective To discuss the effect of microRNA -27a on U251 glioma cells.Methods Over-expression or inhibition of miR -27a in U251 glioma cells were done by transient transfection of miR -27a mim-ics or AMO-27a in vitro.Cell viability was detected by MTT assay .Invasion ability of U251 was detected by tr-answell invasion assay.The level of miR-27a and PPARγwere detected by real -time PCR.Results Under in-hibition of miR-27a condition,the proliferation and invasiveness of U 251 glioma cells were decreased .The level of PPARγwas significantly increased ,whereas the level of miR-27 a was decreased .Overexpression of miR-27 a increased the proliferation and invasiveness of U 251 glioma cells and decreased the level of PPARγ.Conclusion Inhibition of miR-27a is benefit for inhibiting the proliferation and invasiveness of U 251 glioma cells.

3.
China Pharmacy ; (12)2007.
Artigo em Chinês | WPRIM | ID: wpr-533230

RESUMO

OBJECTIVE:To establish the method for sterility test of dexrazoxane for injection. METHODS: The relative procedures were conducted in accordance with the membrane-filter procedure included in sterility test specified in Chinese Pharmacopoeia (2005 edition),with Staphylococcus aureus,Pseudomonas aeruginosa,Bacillus subtilis,Clostridium sporogenes,Candida albicans and Aspergillus niger as test organisms,which were flushed with sodium chloride-peptone buffer solution (150 mL,250 mL,and 350 mL,respectively,pH 7.0) to evaluate the impact of three flushing doses on the results of sterility test. RESULTS: At a flushing dose of 150 mL,there was no growth of bacteria except Bacillus subtilis at 24~72 h;however,at a flushing dose of 250 mL or 350 mL,all bacteria strains had a good growth. CONCLUSION: The established method for sterility test of dexrazoxane for injection is accurate and reliable.

4.
Chinese Traditional Patent Medicine ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-580958

RESUMO

AIM: To determine quantitatively active ingredients,icariine and osthole,and to identify Fructus Cnidium and Scorpio in Ruxinan Capsule ( Folium Epimedii,Fructus Cnidii,Scorpio). METHODS: Fructus Cnidium and Scorpio were identified by TLC and the contents of icariine and osthole were determined by HPLC. RESULTS: Fructus Cnidium and Scorpio were clearly separated in the TLC chromatogram. The results of HPLC analysis for icariine were: a linear range of 61. 7 ng-740. 4 ng,r = 0. 999 6,an average recovery of 99. 86% with a RSD of 0. 82% and the corresponding data for osthole were: a linear range of 57. 4 ng-688. 8 ng,r = 0. 999 8, an average recovery of 99. 55% with a RSD of 0. 71% . CONCLUSION: This method is simple and feasible and can be used as quality standards for Ruxinan Capsule.

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