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To introduce the biological properties and functions of individual blood-brain barrier components,and summarize the most widely used in vitro blood-brain barrier models,compare their strengths and weaknesses,and provide suggestions on model selection in blood-brain barrier research and new-drug research and development.
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Objective To investigate the correlation between neuronal injury and the expression of caspase-3 and caspase-activated deoxyribonuclease (CAD) after focal cerebral ischemia-reperfusion in rats, also to study the effect of caspase-3 particular inhibitor. Methods The focal cerebral ischemia model (occluding middle cerebral artery of the rats) was made by using modified inserting thread method and reperfusion after embolizing for one hour. Using HE staining, TUNEL staining and microscopy to observe the morphological changes of ischemic neurons at six different time points including 6,12,24,48 and 72 h, using immunohistochemistry to observe the changes of caspase-3 and CAD protein in two groups (model group and interfere group). Results There was no significant difference between the two groups using HE staining and microscopy. While there was difference of TUNEL staining positive cells in all time points, except 6 h time point; Both the two groups reached the expression peak of caspase-3 in 24 h, and the number was 2. 360± 0. 318 and 0. 804 ± 0. 206 respectively(t' = 10. 039, P < 0. 01), there was statistical significance from 12 h to 48 h between the two groups ; The expression peak time of CAD protein in two groups was 48 h, and the number was 3.061 ± 0. 567 and 0. 812 ± 0. 240 respectively (t' = 8. 960, P < 0. 01), there was statistical significance from 12 to 72 h between two groups. Conclusions Caspase-3-CAD-DNA degradation is one important way of neuronal injury in cerebral ischemia-reperfusion of rats, caspase-3 inhibitor can protect neuron in a certain degree.
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Objective To study the effect of Thrombolytic Compound (TC) injected into internal carotid at superacute stage.Methods 66 Wistar rats were divided into group A,B and C randomly. Focal cerebral ischemic model was established by improved microthromboembolus method. The rats were intubated conversely into internal carotid 3h after cerebral ischemia for treatment with normal saline (group A),Urokinase(group B) and TC(group C) respectively. The neural functions of these rats were evaluated at 2 h after cerebral ischemia,2.5 and 23.5h after treatment and pathological changes were observed under optic and electron microscopes 24 h following thrombolysis.Results There was no significant difference of neural function scores among the three groups before administration.The scores of group C were lower than those of group A after treatment ( P